A5067794615- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- biodegradable polymer synthesis and properties
- Enzyme-mediated dye degradation
- Studies on Chitinases and Chitosanases
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Microplastics and Plastic Pollution
- Polyamine Metabolism and Applications
- Chemical Synthesis and Reactions
- Biopolymer Synthesis and Applications
- Advanced Cellulose Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Additive Manufacturing and 3D Printing Technologies
- Marine Biology and Environmental Chemistry
Technical University of Denmark
2023-2024
University of Copenhagen
2019-2021
Lytic polysaccharide monooxygenases (LPMOs) have attracted attention due to their ability boost cellulolytic enzyme cocktails for application in biorefineries. However, the interplay between LPMOs and individual glycoside hydrolases remains poorly understood. We investigated how activity of two cellobiohydrolases (Cel7A Cel6A) an endoglucanase (Cel7B) from Trichoderma reesei were affected by a C1-oxidizing LPMO Thielavia terrestris (TtAA9). quantified products mixture hydrolase estimated...
Abstract Enzyme reactions, both in Nature and technical applications, commonly occur at the interface of immiscible phases. Nevertheless, stringent descriptions interfacial enzyme catalysis remain sparse, this is partly due to a shortage coherent experimental data guide assess such work. In work, we produced kinetically characterized 83 cellulases, which revealed conspicuous linear free energy relationship (LFER) between substrate binding strength activation barrier. The scaling occurred...
Bioprocessing of poly(ethylene terephthalate) (PET) waste has emerged as a promising approach to circular economy. The key this development been the discovery enzymes that effectively depolymerize PET its constituent monomers, and hitherto, most candidates are quite closely related, bacterial cutinases. Fungal have less investigated, although few examples hydrolytic activity against reported. To further assess this, we produced 24 fungal cutinases homologues known hydrolases identified three...
The successful enzymatic degradation of polyester substrates has fueled worldwide investigation into the treatment plastic waste using bio-based processes. Within this realm, marine-associated microorganisms have emerged as a promising source polyester-degrading enzymes. In work, we describe hydrolysis synthetic polymer PET by SM14est, polyesterase which was previously identified from Streptomyces sp. SM14, an isolate marine sponge Haliclona simulans. hydrolase activity purified SM14est...
Photobiocatalysis holds great promise toward the development of sustainable and environmentally friendly processes, harnessing light to drive biocatalytic reactions. However, photobiocatalysis at interface insoluble substrates, such as cellulose, has not been studied in much detail. In this context, catalytic enhancement lytic polysaccharide monooxygenases (LPMOs) by is interest biorefinery field due their capacity oxidatively cleave recalcitrant polysaccharides which can facilitate...
Abstract Objective The development of an enzymatic assay for the specific quantification C1-oxidation product, i.e. gluconic acid cellulose active lytic polysaccharide monooxygenases (LPMOs). Results In combination with a β-glucosidase, spectrophotometrical can reliably quantify C1- oxidation product LPMOs acting on cellulose. It is applicable pure model substrate as well lignocellulosic biomass. compares performed by HPAEC-PAD. addition, we show that simple boiling not sufficient to...
Lytic polysaccharide monooxygenases (LPMOs) are known to act synergistically with glycoside hydrolases in industrial cellulolytic cocktails. However, a few studies have reported severe impeding effects of C1-oxidizing LPMOs on the activity reducing-end cellobiohydrolases. The mechanism for this effect remains unknown, but it may important implications as cellobiohydrolases make up significant part such To elucidate whether is general different and study underlying mechanism, we conducted...
Global plastic production exceeded 400 million tons in 2022, urgently demanding improved waste management and recycling strategies for a circular economy. While the enzymatic hydrolysis of polyethylene terephthalate (PET) has become feasible on industrial scales, efficient enzymes targeting other hydrolysable types such as polyurethanes (PURs) are lacking. Recently, amidase signature (AS) family, capable cleaving urethane bonds polyether‐PUR analog linear polyester‐PUR, have been identified....
Global plastic production exceeded 400 million tons in 2022, urgently demanding improved waste management and recycling strategies for a circular economy. While the enzymatic hydrolysis of polyethylene terephthalate (PET) has become feasible on industrial scales, efficient enzymes targeting other hydrolysable types such as polyurethanes (PURs) are lacking. Recently, amidase signature (AS) family, capable cleaving urethane bonds polyether‐PUR analog linear polyester‐PUR, have been identified....
Fungal beta-glucosidases (BGs) from glucoside hydrolase family 3 (GH3) are industrially important enzymes, which convert cellooligosaccharides into glucose; the end product of cellulolytic process. They highly active against β-1,4 glycosidic bond in soluble substrates but typically reported to be inactive insoluble cellulose.We studied activity four fungal GH3 BGs on cellulose and found significant activity. At low temperatures (10 ℃), we derived approximate kinetic parameters kcat = 0.3 ±...
Abstract Enzyme reactions, both in Nature and technical applications, commonly occur at the interface of immiscible phases. Nevertheless, stringent descriptions interfacial enzyme catalysis remain sparse, this is partly due to a shortage coherent experimental data guide assess such work. We have produced kinetically characterized 83 cellulases, which revealed conspicuous linear free energy relationship (LFER) between strength substrate binding activation barrier. This common scaling occurred...