A5010321204- Biofuel production and bioconversion
- Lignin and Wood Chemistry
- Enzyme-mediated dye degradation
- Advanced Cellulose Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Catalysis for Biomass Conversion
- Bioenergy crop production and management
- Enzyme Catalysis and Immobilization
- Food composition and properties
- Biochemical and biochemical processes
- Polysaccharides and Plant Cell Walls
- Enzyme Production and Characterization
- Global Energy Security and Policy
- Fermentation and Sensory Analysis
- Spectroscopy and Chemometric Analyses
- Hybrid Renewable Energy Systems
- Global Energy and Sustainability Research
- Wood Treatment and Properties
- Natural Fiber Reinforced Composites
- Energy and Environment Impacts
- Environmental Impact and Sustainability
- Anaerobic Digestion and Biogas Production
- Cultural Heritage Materials Analysis
- Dyeing and Modifying Textile Fibers
- Bioeconomy and Sustainability Development
University of Copenhagen
2014-2023
Novo Nordisk Foundation
2021-2022
Frederiksberg Hospital
2019
Svebio
2006
Ministry of Agriculture
2006
Estonian University of Life Sciences
2006
World Bioenergy Association
2006
Auburn University
2003
Novozymes (Denmark)
2001-2002
Novo Nordisk (Denmark)
2000
Abstract The economic dependency on fossil fuels and the resulting effects climate environment have put tremendous focus utilizing fermentable sugars from lignocellulose, largest known renewable carbohydrate source. in lignocellulose are derived cellulose hemicelluloses but these not readily accessible to enzymatic hydrolysis require a pretreatment, which causes an extensive modification of lignocellulosic structure. A number pretreatment technologies under development being tested pilot...
Working at high solids (substrate) concentrations is advantageous in enzymatic conversion of lignocellulosic biomass as it increases product and plant productivity while lowering energy water input. However, for a number substrates has been shown that increasing substrate concentration, the corresponding yield decreases fashion which can not be explained by current models knowledge enzyme-substrate interactions. This decrease undesirable offsets advantages working levels. The cause 'solids...
Abstract To improve process economics of the lignocellulose to ethanol a reactor system for enzymatic liquefaction and saccharification at high‐solids concentrations was developed. The technology is based on free fall mixing employing horizontally placed drum with horizontal rotating shaft mounted paddlers mixing. Enzymatic pretreated wheat straw tested up 40% (w/w) initial DM. In less than 10 h, structure material changed from intact particles (length 1–5 cm) into paste/liquid that could be...
Pretreatment is an essential step in the enzymatic hydrolysis of biomass and subsequent production bioethanol. Recent results indicate that only a mild pretreatment necessary industrial, economically feasible system. The Integrated Biomass Utilisation System hydrothermal process has previously been shown to be effective preparing wheat straw for these processes without application additional chemicals. In current work, effect on cell-wall matrix its components are characterised...
The recent discovery of accessory proteins that boost cellulose hydrolysis has increased the economical and technical efficiency processing to bioethanol. Oxidative enzymes (e.g. GH61) present in new commercial enzyme preparations have shown increase conversion yields. When using pure substrates it been determined both oxidized unoxidized cellodextrin products are formed. We report effect oxidative activity a mix (Cellic CTec2) upon overall hydrolysis, formation impact on β-glucosidase...
Abstract Enzymatic oxidation of cell wall polysaccharides by lytic polysaccharide monooxygenases (LPMOs) plays a pivotal role in the degradation plant biomass. While experiments have shown that LPMOs are copper dependent enzymes requiring an electron donor, mechanism and origin supply biological systems only partly understood. We show here insoluble high molecular weight lignin functions as reservoir electrons facilitating LPMO activity. The donated to enzyme long-range transfer involving...
Abstract Oxidative processes are essential for the degradation of plant biomass. A class powerful and widely distributed oxidative enzymes, lytic polysaccharide monooxygenases (LPMOs), oxidize most recalcitrant polysaccharides require extracellular electron donors. Here we investigated effect using excited photosynthetic pigments as LPMOs combined with reducing agents were exposed to light, which resulted in a never before seen 100-fold increase catalytic activity. In addition, LPMO...
Biological degradation of biomass on an industrial scale culminates in high concentrations end products. It is known that the accumulation glucose and cellobiose, products hydrolysis, inhibit cellulases decrease yields. Aside from these products, however, other monosaccharides such as mannose galactose (stereoisomers glucose) yields well. NMR relaxometry measurements showed direct correlations between initial T2 liquid phase which hydrolysis takes place total production during cellulose...
Sugarcane bagasse, corn stover, and wheat straw are among the most available resources for production of cellulosic ethanol.
The discovery of lignin as activator for the redox enzyme lytic polysaccharide monooxygenases (LPMOs) oxidation cell-wall polysaccharides opens a new scenario investigation interplay between different lignocellulose-degrading enzymes. lignin-active enzymes in one hand, and carbohydrate active other, are linked through variety electrons carrier molecules either derived from or enzymatically transferred. Likewise, nature, many organisms expressing those simultaneously, we wanted to test if...
Enzymatic oxidation of lignin phenolic hydroxyl groups can enhance the level auto adhesion between wood fibers. Previous investigations within area have not taken adhesive effect proteins and carbohydrates in enzyme solution into consideration, part claimed generated may merely be a result solution. In this paper laccase is used to oxidize beech (Fagus sylvatica) Laccase catalyzes one-electron while reducing oxygen, yielding phenoxy radicals water. Wet dry process fiberboards are made from...