A5003676223- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Carbohydrate Chemistry and Synthesis
- Biofuel production and bioconversion
- Metabolomics and Mass Spectrometry Studies
- Fungal and yeast genetics research
- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Microbial Metabolites in Food Biotechnology
- Galectins and Cancer Biology
- Fungal Biology and Applications
- Fermentation and Sensory Analysis
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Enzyme Catalysis and Immobilization
- Food composition and properties
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- Cell Adhesion Molecules Research
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Diet, Metabolism, and Disease
- Phytase and its Applications
- Lignin and Wood Chemistry
VTT Technical Research Centre of Finland
2014-2025
Tieto (Finland)
2014
University of Helsinki
1992-2011
Åbo Akademi University
2008
University at Buffalo, State University of New York
2001-2004
Helsinki Institute of Physics
1997-2001
BOKU University
2000
University of Lübeck
2000
Boston University
1997
Biocenter Finland
1997
The phytohormones jasmonates (JAs) constitute an important class of elicitors for many plant secondary metabolic pathways. However, JAs do not act independently but operate in complex networks with crosstalk to several other phytohormonal signaling Here, was detected between the JA and abscisic acid (ABA) pathways regulation tobacco ( Nicotiana tabacum ) alkaloid biosynthesis. A gene from PYR/PYL/RCAR family, NtPYL4 , expression which is regulated by JAs, found encode a functional ABA...
Abstract Background Yeasts are attractive expression platforms for many recombinant proteins, and there is evidence an important interrelation between the protein secretion machinery environmental stresses. While adaptive responses to such stresses extensively studied in Saccharomyces cerevisiae , little known about their impact on physiology of Pichia pastoris . We have recently reported a beneficial effect hypoxia Fab P. chemostat cultivations. As consequence, systems biology approach was...
Abstract Background The methylotrophic yeast Pichia pastoris has emerged as one of the most promising hosts for production heterologous proteins. Mixed feeds methanol and a multicarbon source instead sole carbon have been shown to improve product productivities alleviate metabolic burden derived from protein production. Nevertheless, systematic quantitative studies on relationships between central metabolism recombinant in P. are still rather limited, particularly when growing this mixed...
Aerobic and anaerobic central metabolism of Saccharomyces cerevisiae cells was explored in batch cultures on a minimal medium containing glucose as the sole carbon source, using biosynthetic fractional 13 C labeling proteinogenic amino acids. This allowed, firstly, unravelling network active pathways cytosol mitochondria, secondly, determination flux ratios characterizing glycolysis, pentose phosphate cycle, tricarboxylic acid cycle C1‐metabolism, thirdly, assessment intercompartmental...
The yeast Saccharomyces cerevisiae is able to adjust external oxygen availability by utilizing both respirative and fermentative metabolic modes. Adjusting the mode involves alteration of intracellular fluxes that are determined cell's multilevel regulatory network. Oxygen a major determinant physiology S. but understanding dependence flux distributions still scarce. Metabolic CEN.PK113-1A growing in glucose-limited chemostat cultures at dilution rate 0.1 h-1 with 20.9%, 2.8%, 1.0%, 0.5% or...
An NAD(+)-dependent D-xylose dehydrogenase, XylB, from Caulobacter crescentus was expressed in Saccharomyces cerevisiae, resulting production of 17 ± 2 g D-xylonate l(-1) at 0.23 gl(-1)h(-1) 23 (with glucose and ethanol as co-substrates). D-Xylonate titre rate were increased xylitol decreased, compared to strains expressing genes encoding T. reesei or pig liver NADP(+)-dependent dehydrogenases. accumulated intracellularly ∼70 mgg(-1); ∼18 mgg(-1). The aldose reductase gene GRE3 deleted...
Abstract The limited access to fast and facile general analytical methods for cellulosic and/or biocomposite materials currently stands as one of the main barriers progress these disciplines. To that end, a diverse set narrow techniques are typically employed often time-consuming, costly, not necessarily available on daily basis practitioners. Herein, we rigorously demonstrate quantitative NMR spectroscopic method structural determination crystalline cellulose samples. Our relies use readily...
The P-selectin counterreceptor PSGL-1 is covalently modified by mono alpha2,3-sialylated, multiply alpha1,3-fucosylated polylactosamines. These glycans are required for the adhesive interactions that allow this adhesion receptor-counterreceptor pair to facilitate leukocyte extravasation. To begin understand biosynthesis of these glycans, we have characterized acceptor and site specificities two granulocyte alpha1,3-fucosyltransferases, Fuc-TIV Fuc-TVII, using recombinant forms enzymes a...
Amino acid biosynthesis and central carbon metabolism of Pichia pastoris were studied using biosynthetically directed fractional 13 C labeling. Cells grown aerobically in a chemostat culture fed at two dilution rates (0.05 h −1 , 0.16 ) with glycerol as the sole source. For investigation amino comparison cultivations, cells also on glucose. Our results show that, firstly, acids are synthesized Saccharomyces cerevisiae . Secondly, mitochondrial pyruvate via malic enzyme is not registered for...
The metabolic pathways associated with the tricarboxylic acid cycle intermediates of Pichia pastoris were studied using biosynthetically directed fractional 13C labelling. Cells grown aerobically in a chemostat culture fed at two dilution rates (1.39×10−5 s−1 and 4.44×10−5 s−1) varying mixtures glycerol methanol as sole carbon sources. results show that, co-assimilation methanol, common amino acids are synthesized P. cells on only. During growth lower rate, when both substrates entirely...
The migration of acetyl, pivaloyl, and benzoyl protective groups their relative stabilities at variable pH for a series β-d-galactopyranoses were studied by NMR spectroscopy. clockwise counterclockwise rates the different ester accurately determined use kinetic model. results presented provide new insights into acid base commonly used protecting phenomenon acyl group may prove useful in planning synthesis strategies.
Saccharomyces cerevisiae CEN.PK113-1A was grown in glucose-limited chemostat culture with 0%, 0.5%, 1.0%, 2.8% or 20.9% O2 the inlet gas (D=0.10 h(-1), pH 5, 30 degrees C) to determine effects of oxygen on 17 metabolites and 69 genes related central carbon metabolism. The concentrations tricarboxylic acid cycle (TCA) all glycolytic except 2-phosphoglycerate+3-phosphoglycerate phosphoenolpyruvate were higher anaerobic than fully aerobic conditions. Provision only 0.5-1% reduced most...
Chemical changes of lignin induced by the steam explosion (SE) process were elucidated. Wheat straw was studied as raw material, and lignins isolated enzymatic mild acidolysis (EMAL) procedure before after SE treatment for analyses mainly two-dimensional (2D) [heteronuclear single-quantum coherence (HSQC) heteronuclear multiple-bond correlation (HMBC)] 31P nuclear magnetic resonance (NMR). The β-O-4 structures found to be homolytically cleaved, followed recoupling β-5 linkages. homolytic...
O-Acetylglucuronoxylans (AcGX) in Arabidopsis thaliana carry acetyl residues on the 2-O and/or 3-O positions of xylopyranosyl (Xylp) units, but distribution different O-acetylated Xylp units is partly unclear. We studied a possible correlation xylan acetylation and activities glycosyltransferases involved biosynthesis by analyzing O-acetyl substituents AcGX from wild-type mutants irx7, irx9-1, irx10, irx14 gux1gux2. The relative contents structural were determined with quantitative...
Anthraquinones in the emodin family are produced by bacteria, fungi, and plants. They display various biological activities exploited, e.g., for crop protection, may also be utilized as sustainable, bio-based colorants textile, paints, electronics, cosmetic industries. Anthraquinone pigments from Cortinarius mushrooms have been used artisan dyeing because they stable, colorfast, compatible with methods. However, their chemical synthesis is complex uneconomical, harvesting wild forests...
Weissella confusa VTT E-90392 is an efficient producer of a dextran that mainly composed α-(1→6)-linked D-glucosyl units and very few α-(1→3) branch linkages. A mixture the Chaetomium erraticum endodextranase Aspergillus niger α-glucosidase was used to hydrolyze W. glucose set enzyme-resistant isomaltooligosaccharides. Two oligosaccharides (tetra- hexasaccharide) were isolated in pure form their structures elucidated. The tetrasaccharide had nonreducing end terminal α-(1→3)-linked glucosyl...
Lysostaphin from Staphylococcus simulans and its family enzymes rapidly acquire prominence as the next generation agents in treatment of S. aureus infections. The specificity lysostaphin is promoted by C-terminal cell wall targeting domain selectivity toward pentaglycine bridges wall. Scission these cross-links carried out N-terminal catalytic domain, a zinc-dependent endopeptidase. Understanding determinants affecting efficiency catalysis strength interactions lies at heart all enzyme...
The recognition of cell‐surface l ‐selectin by its carbohydrate ligands causes lymphocytes to roll on capillary endothelium at sites inflammation. As this primary contact is a prerequisite for extravasation the leukocytes tissue, inhibition free oligosaccharides capable competing with natural an attractive therapeutic possibility. exact structures biological are not yet known, but principal epitopes share some structural features: they O‐glycosidically linked mucin‐type N ‐acetyllactosamine...
Methyl jasmonate is capable of initiating or improving the biosynthesis secondary metabolites in plants and therefore has opened up a concept for valuable constituents. In this study, effect different doses methyl (MeJA) elicitation on accumulation terpenoid indole alkaloids (TIAs) hairy root cultures medicinal plant, Rhazya stricta throughout time course (one-seven days) was investigated. Gas chromatography-mass spectrometry (GC-MS) analyses were carried out targeted ten major non-polar...
Abstract In this work, deoxyribose-5-phosphate aldolase ( Ec DERA, EC 4.1.2.4) from Escherichia coli was chosen as the protein engineering target for improving substrate preference towards smaller, non-phosphorylated aldehyde donor substrates, in particular acetaldehyde. The initial broad set of mutations directed to 24 amino acid positions active site or close vicinity, based on 3D complex structure E. DERA wild-type aldolase. specific activity variants containing one three characterised...