A5012595187- Plant tissue culture and regeneration
- Plant-derived Lignans Synthesis and Bioactivity
- Transgenic Plants and Applications
- Inorganic and Organometallic Chemistry
- Chemical Synthesis and Reactions
- Allelopathy and phytotoxic interactions
- Chemical Synthesis and Analysis
- Phytochemistry and Biological Activities
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Organic Chemistry Cycloaddition Reactions
- Plant biochemistry and biosynthesis
- Asymmetric Synthesis and Catalysis
- Toxin Mechanisms and Immunotoxins
- Synthesis and Reactions of Organic Compounds
- Food Industry and Aquatic Biology
- Traditional and Medicinal Uses of Annonaceae
- Pharmacogenetics and Drug Metabolism
- Fluorine in Organic Chemistry
- Synthesis and Characterization of Heterocyclic Compounds
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Weed Control and Herbicide Applications
- Biochemical Analysis and Sensing Techniques
- GABA and Rice Research
- Eicosanoids and Hypertension Pharmacology
- Cannabis and Cannabinoid Research
TU Dortmund University
2021
Fraunhofer Institute for Molecular Biology and Applied Ecology
2014-2016
Fraunhofer Society
2013-2015
Medical University of Sofia
2004-2008
University of Groningen
2006
Bandung Institute of Technology
2006
Sofia University "St. Kliment Ohridski"
2004
A.E. Favorsky Irkutsk Institute of Chemistry
1966-1972
Abstract Multiple factors act simultaneously on plants to establish complex interaction networks involving nutrients, elicitors and metabolites. Metabolomics offers a better understanding of biological systems, but evaluating the simultaneous impact different parameters metabolic pathways that have many components is challenging task. We therefore developed novel approach combines experimental design, untargeted profiling based multiple chromatography systems ionization modes, multiblock...
We have developed a strategy for the optimization of plant cell suspension culture media using combination fractional factorial designs (FFDs) and response surface methodology (RSM). This sequential approach was applied to transformed tobacco BY-2 cells secreting human antibody (M12) into medium, in an effort maximize yields. found that nutrients KNO₃, NH₄NO₃ CaCl₂ hormones 2,4-dichlorophenoxyacetic acid (2,4-D) 6-benzylaminopurine (BAP) had most significant impact on accumulation. The...
A large-scale statistical experimental design was used to determine essential cultivation parameters that affect biomass accumulation and geraniol production in transgenic tobacco (Nicotiana tabacum cv. Samsun NN) cell suspension cultures. The carbohydrate source played a major role determining the yield factors such as filling volume, inoculum size light were less important. Sucrose, volume had positive effect on by boosting growth of plant cultures whereas illumination stimulated...
The aryltetralin lignans 6-methoxypodophyllotoxin, 5′-demethoxy-6-methoxypodophyllotoxin as well the corresponding 8′-epimers 6-methoxypicropodophyllin, and 5′-demethoxy-6-methoxypicropodophyllin were isolated from suspension cultures of Linum cariense, 4′-demethyl-6-methoxypodophyllotoxin together with 6-methoxypodophyllotoxin plants L. tauricum, which both belong to section Syllinum genus Linum. Cell altaicum, austriacum ssp. euxinum lewisii belonging accumulate naphthalene justicidin B...
Callus and hairy root cultures of Linum leonii were established. The genetic transformation in roots was proven by PCR analysis, which showed integration rol A C genes into the plant genome. Calli accumulate arylnaphthalene lignan justicidin B as a major constituent. Hairy produce 5-fold higher yields (10.8 mg g-1 DW) compared to calli. Justicidin shows strong cytotoxicity on chronic myeloid leukemia LAMA-8 K-562 cell lines lymphoid SKW-3 line with IC50 values 1.11, 6.08, 1.62 μM,...
Lignans in eighteen samples of Linum species (L. tauricum ssp. tauricum, serbicum, bulgaricum and linearifolium; L. elegans; flavum sparsiflorum, capitatum var. laxiflorum), all members the section Syllinum occurring Bulgaria, were analysed by HPLC-ESI/MS HPLC-UV/DAD. The ESI/MS fragmentation pathways recently established for aryltetralin lignans are now extended to ester glycoside derivatives. In total, 22 different lignans, mainly type, identified. 6-Methoxypodophyllotoxin its glucoside...
Callus and suspension cultures of Linum campanulatum. L. setaceum. Brot. (Linaceae) were established to study accumulation lignans. Justicidin B proved be the main constituent in callus Phytochemical analysis cell has demonstrated that produces yatein, desoxypodophyllotoxin, β.-peltatin, whereas contain 6-methoxypodophyllotoxin.