A5051567910- Fungal and yeast genetics research
- Galectins and Cancer Biology
- Transgenic Plants and Applications
- Glycosylation and Glycoproteins Research
- Viral Infectious Diseases and Gene Expression in Insects
- Endoplasmic Reticulum Stress and Disease
- Biofuel production and bioconversion
- Toxin Mechanisms and Immunotoxins
- Microbial Metabolic Engineering and Bioproduction
- Monoclonal and Polyclonal Antibodies Research
- Cellular transport and secretion
- RNA modifications and cancer
- Biochemical and biochemical processes
- Advanced Cellulose Research Studies
- biodegradable polymer synthesis and properties
- Lysosomal Storage Disorders Research
- Phytochemistry and Bioactivity Studies
- Plant biochemistry and biosynthesis
- Polysaccharides and Plant Cell Walls
- Fermentation and Sensory Analysis
- Carbohydrate Chemistry and Synthesis
- Pharmacological Effects of Natural Compounds
- Fungal Biology and Applications
VTT Technical Research Centre of Finland
2020-2025
Tieto (Finland)
2020
Aalto University
2014-2017
Almac (United Kingdom)
2017
Radboud University Nijmegen
2011
Radboud University Medical Center
2011
Anthraquinones in the emodin family are produced by bacteria, fungi, and plants. They display various biological activities exploited, e.g., for crop protection, may also be utilized as sustainable, bio-based colorants textile, paints, electronics, cosmetic industries. Anthraquinone pigments from Cortinarius mushrooms have been used artisan dyeing because they stable, colorfast, compatible with methods. However, their chemical synthesis is complex uneconomical, harvesting wild forests...
The yeast Saccharomyces cerevisiae provides intriguing possibilities for synthetic biology and bioprocess applications, but its use is still constrained by cellular characteristics that limit the product yields. Considering production of advanced biopharmaceuticals, a major hindrance lies in endoplasmic reticulum (ER), as it not equipped efficient large scale folding complex proteins, such human antibodies. Following example professional secretory cells, we show inducing an ER expansion...
Abstract The cellular changes induced by heterologous protein expression in the yeast Saccharomyces cerevisiae have been analysed on many levels and found to be significant. However, even though high‐level production poses a metabolic burden, evaluation of host at level metabolome has often neglected. We present comparison metabolite profiles wild‐type strain with those three strains producing recombinant antibody variants increasing size complexity: an scFv fragment, scFv–Fc fusion...
In nature, various organisms produce cellulose as microfibrils, which are processed into their nano- and microfibrillar and/or crystalline components by humans in order to obtain desired material properties. Interestingly, the natural synthesis machinery can be circumvented enzymatically synthesizing from precursor molecules vitro. This approach is appealing for producing tailor-made cellulosic particles materials because it enables optimization of reaction conditions generate with a...
Replacement of petrochemical-based materials with microbially produced biodegradable alternatives calls for industrially attractive fermentation processes. Lignocellulosic offer non-edible cultivated sugars, but require often use expensive sugar releasing enzymes, such as β-glucosidases. These cellulose treatment costs could be reduced if microbial production hosts short cellodextrins cellobiose directly their substrates. In this study, we demonstrate poly(hydroxybutyrate) (PHB) in yeast...
Abstract The so-called “craft beer revolution” has increased the demand for new styles of beers, often with ingredients like flavour extracts. In recent years, synthetic biology realized production a plethora plant secondary metabolites in microbial hosts, which could provide an alternative source these compounds. this study, we selected situ approach grape addition. We used O -methyl anthranilate (OmANT) producing laboratory Saccharomyces cerevisiae strain co-fermentations industrial yeast...
Processed lignocellulosic biomass is a source of mixed sugars that can be used for microbial fermentation into fuels or higher value products, like chemicals. Previously, the yeast Saccharomyces cerevisiae was engineered to utilize its cellodextrins through heterologous expression sugar transporters together with an intracellular expressed β-glucosidase. In this study, we screened selection eight (putative) cellodextrin from different and fungal hosts in order extend catalogue available...
Cost-effective manufacturing of biopharmaceuticals in non-mammalian hosts still requires tremendous efforts strain development. In order to expedite identification novel leads for engineering, we used a transposon-mutagenized yeast genomic DNA library create collection Saccharomyces cerevisiae deletion strains expressing full-length IgG antibody. Using high-throughput screening, transformants with either significantly higher or lower expression were selected. The integration site the...
Therapeutic protein production in yeast is a reality industry with an untapped potential to expand more complex proteins, such as full-length antibodies. Despite numerous engineering approaches, cellular limitations are preventing the use of Saccharomyces cerevisiae titers recombinant antibodies currently not competitive. Instead host specific approach, possibility adopting features from native producers antibodies, plasma cells, improve antibody yeast. A subset mammalian folding factors...