Various selection procedures in chemostats and batch cultures were systematically tested for their efficiency to select a multiple-stress resistance phenotype Saccharomyces cerevisiae. To determine the relative stress phenotypes, mutant populations harvested at different time points randomly chosen clones from selected grown exposed oxidative, freezing–thawing, high-temperature ethanol stress. For this purpose, we developed high-throughput procedure 96-well plates combined with...

2-Phenylethanol is an aromatic compound commonly used in the food, cosmetic, and pharmaceutical industries. Due to increasing demand for natural products by consumers, production of this flavor microbial fermentation gaining interest, as a sustainable alternative chemical synthesis or expensive plant extraction, both processes relying on use fossil resources. However, drawback process high toxicity 2-phenylethanol producing microorganism. The aim study was obtain 2-phenylethanol-resistant...

Aerobic and anaerobic central metabolism of Saccharomyces cerevisiae cells was explored in batch cultures on a minimal medium containing glucose as the sole carbon source, using biosynthetic fractional 13 C labeling proteinogenic amino acids. This allowed, firstly, unravelling network active pathways cytosol mitochondria, secondly, determination flux ratios characterizing glycolysis, pentose phosphate cycle, tricarboxylic acid cycle C1‐metabolism, thirdly, assessment intercompartmental...

ABSTRACT The so far largely uncharacterized central carbon metabolism of the yeast Pichia stipitis was explored in batch and glucose-limited chemostat cultures using metabolic-flux ratio analysis by nuclear magnetic resonance. concomitantly characterized network active metabolic pathways compared to those identified Saccharomyces cerevisiae , which led following conclusions. (i) There is a remarkably low use non-oxidative pentose phosphate (PP) pathway for glucose catabolism S. when P....

Summary One of the challenges with sperm DNA fragmentation results is inconsistency and large variability in obtained by different techniques. The terminal deoxynucleotidyl transferase d UTP nick end labeling (TUNEL) assay quantifies incorporation fluoresceinated into single‐ double‐strand breaks 3′‐ OH TdT. goal this study was optimize TUNEL protocol for assessment standardization method comparison data across two reference laboratories (i) at Basel, Switzerland (ii) Cleveland Clinic, Ohio,...

Oxidative stress is a major type observed in yeast bioprocesses, resulting decrease growth, viability, and productivity. Thus, robust strains with increased resistance to oxidative are highly demand by the industry. In addition, also associated aging age-related complex conditions such as cancer neurodegenerative diseases. Saccharomyces cerevisiae, model eukaryote, has been used study these eukaryotic processes. However, molecular mechanisms underlying responses unclear. this study, we have...

ABSTRACT Phenolic inhibitors in lignocellulosic hydrolysates interfere with the performance of fermenting microorganisms. Among these, coniferyl aldehyde is one most toxic inhibitors. In this study, genetically stable Saccharomyces cerevisiae mutants high resistance were successfully obtained for first time by using an evolutionary engineering strategy, based on systematic application increasing stress batch cultures. selected aldehyde-resistant mutants, highly resistant strain called BH13...

Propolis is a highly complex, resinous natural product collected by honeybees from tree leaves and buds mixed with pollen enzymes. Due to its antimicrobial properties, it has various medical industrial applications. As nonconventional strategy, the use of propolis was suggested control contaminating yeast growth in ethanol fermentations, without significantly affecting starter fermentation, Saccharomyces cerevisiae. In this study, we have developed propolis-resistant S. cerevisiae strain...

High acrylamide (ACR) content in heat-processed carbohydrate-rich foods, as well roasted products such coffee, almonds etc., has been found to be a risk factor for carcinogenicity and genotoxicity by The World Health Organization. Glycidamide (GLY), the epoxide metabolite of ACR, is processed cytochrome P-450 enzyme system also genotoxic agent. aim this study was determine whether ACR and/or GLY have any detrimental effect on meiotic cell division oocytes. For purpose, germinal vesicle-stage...

Increased exposure to nickel compounds and alloys due industrial development has resulted in pollution many pathological effects on human health. However, there is very limited information about response, transport, tolerance eukaryotes. To investigate resistance the model eukaryote Saccharomyces cerevisiae, evolutionary engineering by batch selection under gradually increasing stress levels was performed. Nickel hyper-resistant mutants that could resist up 5.3 mM NiCl2 , a lethal level for...

Oxidative stress is a significant risk factor for male infertility. A pro-oxidant testicular environment may alter the expression profile of functional sperm proteins and result in poor quality. Patients donors were divided into ROS (-) (+) groups. Using computational studies, data mining available literature on spermatozoa, oxidative proteomics, we identified three core regulatory angiotensin-converting enzyme (ACE), heat-shock protein (Hsp70) family member 2 (HSPA2) ribosomal subunit 27A...

Iron plays an essential role in all organisms and is involved the structure of many biomolecules. It also regulates Fenton reaction where highly reactive hydroxyl radicals occur. important for microbial biodiversity, health nutrition. Excessive iron levels can cause oxidative damage cells. Saccharomycescerevisiae evolved mechanisms to regulate its levels. To study stress resistance S. cerevisiae, evolutionary engineering was employed. The stress-resistant mutant "M8FE" analysed...

Silver is a non-essential metal used in medical applications as an antimicrobial agent, but it also toxic for biological systems. To investigate the molecular basis of silver resistance yeast, we employed evolutionary engineering using successive batch cultures at gradually increased stress levels up to 0.25-mM AgNO3 29 populations and obtained highly silver-resistant genetically stable Saccharomyces cerevisiae strains. Cross-resistance analysis results indicated that mutants gained against...

Introduction: The fungal priority pathogen Cryptococcus neoformans causes cryptococcal meningoencephalitis in immunocompromised individuals and leads to hundreds of thousands deaths per year. undesirable side effects existing treatments, the need for long application times prevent disease from recurring, lack resources these treatment methods spread over all continents necessitate search new methods. Methods: Genome-scale models have been shown be valuable studying metabolism many organisms....

Cobalt is an important metal ion with magnetic properties that widely used for several industrial applications. Overexposure to cobalt ions can be highly toxic the organisms because they usually overwhelm endogenous physiological system maintains their homeostasis causing (geno)toxic effects. To gain insight into mechanism of toxicity, we characterized at molecular and genetic levels a resistant CI25E Saccharomyces cerevisiae strain previously isolated by in vivo evolutionary engineering...