A5043560729- Biofuel production and bioconversion
- Advanced Cellulose Research Studies
- Lignin and Wood Chemistry
- Catalysis for Biomass Conversion
- Enzyme-mediated dye degradation
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Fermentation and Sensory Analysis
- Surfactants and Colloidal Systems
- Protein Structure and Dynamics
- Magnetic and Electromagnetic Effects
- Polymer Nanocomposites and Properties
- Polysaccharides Composition and Applications
- Rheology and Fluid Dynamics Studies
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Proteins in Food Systems
University of Copenhagen
2012-2023
University of Calgary
2020
University of British Columbia
2008-2012
The recent discovery of accessory proteins that boost cellulose hydrolysis has increased the economical and technical efficiency processing to bioethanol. Oxidative enzymes (e.g. GH61) present in new commercial enzyme preparations have shown increase conversion yields. When using pure substrates it been determined both oxidized unoxidized cellodextrin products are formed. We report effect oxidative activity a mix (Cellic CTec2) upon overall hydrolysis, formation impact on β-glucosidase...
Abstract To date, there is limited knowledge available regarding the key features of pretreated lignocellulosic substrates that promote effective enzymatic hydrolysis cellulose component to glucose during bioconversion processes produce ethanol. Fundamentally, cellulase enzymes require access carry out hydrolysis. Porosity and overall surface area have major structural influencing by cellulases. Simons' Stain (SS) a potentially useful semiquantitative method for estimating substrates. In...
Biological degradation of biomass on an industrial scale culminates in high concentrations end products. It is known that the accumulation glucose and cellobiose, products hydrolysis, inhibit cellulases decrease yields. Aside from these products, however, other monosaccharides such as mannose galactose (stereoisomers glucose) yields well. NMR relaxometry measurements showed direct correlations between initial T2 liquid phase which hydrolysis takes place total production during cellulose...
Abstract Milliliter scale (ligno)cellulose saccharifications suggest general solute concentration and its impact on water availability plays a significant role in detrimental effects associated with high solids lignocellulose conversions. A microtumbler developed to enable free‐fall mixing at dry loadings up 35% (w/w) repeatedly produced known conversion trends cellulose, xylan pretreated commercial enzymes. Despite this, concentrations of insoluble nonhydrolysable dextrans did not depress...
Non-ionic surfactants such as polyethylene glycol (PEG) can increase the glucose yield obtained from enzymatic saccharification of lignocellulosic substrates. Various explanations behind this effect include ability PEG to stability cellulases, decrease non-productive cellulase adsorption substrate, and desorption enzymes substrate. Here, using lignin-free model substrates, we propose that also alters solvent properties, for example, water, leading cellulases hydrolysis yields.The differs...
Abstract The chemical characteristics of wheat straw lignin pretreated under dilute acid conditions were compared. After pretreatment, the content solid residue increased as temperature (from 160°C to 190°C) and with amount added (0%, 0.25%, or 1% H 2 SO 4 ). Pretreatment at 190°C increasing concentrations catalyst led a decrease in glucan content, whereas remained almost constant pretreatment regardless concentration. xylan decreased proportion concentration temperature. residual lignins...