- Biofuel production and bioconversion
- Enzyme-mediated dye degradation
- Advanced Cellulose Research Studies
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Plant Pathogenic Bacteria Studies
- Plant Stress Responses and Tolerance
- Plant responses to elevated CO2
- Microbial Metabolism and Applications
- Microbial Fuel Cells and Bioremediation
- Protist diversity and phylogeny
- Lignin and Wood Chemistry
- Legume Nitrogen Fixing Symbiosis
- Catalysis for Biomass Conversion
- Electrochemical sensors and biosensors
Université Libre de Bruxelles
2020-2024
University of British Columbia
2022
Purdue University West Lafayette
2022
Agro-Environmental Protection Institute
2022
Nanjing Agricultural University
2022
Universidad Autónoma de Coahuila
2022
Arizona State University
2022
Indian Institute of Technology Kharagpur
2022
Biochemical Society
2022
University College London
2022
Copper-dependent lytic polysaccharide monooxygenases (LPMOs) classified in Auxiliary Activity (AA) families are considered indispensable as synergistic partners for cellulolytic enzymes to saccharify recalcitrant lignocellulosic plant biomass. In this study, we characterized two fungal oxidoreductases from the new AA16 family. We found that
Lytic Polysaccharide Monooxygenases (LPMOs) are powerful redox enzymes able to oxidatively cleave recalcitrant polysaccharides. Widely conserved across biological kingdoms, LPMOs of the AA9 family deployed by phytopathogens deconstruct cellulose polymers. In response, plants have evolved sophisticated mechanisms sense cell wall damage and thus self-triggering Damage Triggered Immunity responses. Here, we show that Arabidopsis exposed LPMO products triggered innate immunity ultimately leading...
Light-driven enzymatic degradation of lignocellulose could help boosting the economy biorefineries by either shortening bioprocessing time, or accelerating kinetics and so possibly spare some precious enzymes. The redox Lytic Polysaccharide Monooxygenases - LPMOs, a key enzyme for cellulose degradation, had been shown to benefit from photobiocatalysis settings thanks in-situ photocatalytic reduction O2 into H2O2, co-substrate perhaps through faster its catalytically active metal Cu-II Cu-I....
Abstract Melanin is a class of hetero‐polymer pigments commonly found in nature and widely fungi. Often referred to as the “animal lignin“, melanin very abundant bioresource features many catalytically interesting properties. We conceived that, upon light absorbance, polymer could promote long‐distance electron donation fuel redox enzymatic catalysis or controlled in‐situ generation H 2 O . Here, we report on fungal photo‐biocatalytic system extracted from commercially relevant A. nidulans ,...
Global climate change, especially heatwaves and aridity, is a major threat to agricultural production food security. This requires common efforts from the scientific community find effective solutions better understand protect plant's vulnerabilities high temperatures. The current study demonstrates potential of cellooligosaccharides (COS), which are native oxidized signaling molecules released by lytic polysaccharide monooxygenases (LPMO) enzymes during cell wall degradation microbial...
Abstract Lytic Polysaccharide Monooxygenases (LPMOs) are powerful redox enzymes able to oxidatively cleave cellulose polymers. Widely conserved across biological kingdoms, LPMOs of the AA9 family deployed by phytopathogens during necrotrophic attack plant cell wall. In response, plants have evolved sophisticated mechanisms sense wall damage and thus self-triggering Damage Triggered Immunity (DTI) responses. Here, we show that Arabidopsis exposed LPMO products responds activating innate...
Lytic Polysaccharide MonoOxygenases display great variability towards cellulose ultrastructure while using these for carboxylation/functionalization of the polymers. Aiming at employing AA9 LPMOs enzymes isolation nano-crystals (CNCs), functionalization/crystalline degradation ratio becomes crucial. Here we investigated constrains posed by substrate on activity seven different representative various regioselectivity and affinity: TtAA9E, TaAA9A, PcAA9D, MtAA9A, MtAA9D, MtAA9I-CBM MtAA9J. We...