A5066322863- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Plant pathogens and resistance mechanisms
- CRISPR and Genetic Engineering
- Plant Reproductive Biology
- Plant Physiology and Cultivation Studies
- Nematode management and characterization studies
- Transgenic Plants and Applications
- Plant Stress Responses and Tolerance
- RNA modifications and cancer
- Plant Parasitism and Resistance
- Advanced Cellulose Research Studies
- Horticultural and Viticultural Research
- African Botany and Ecology Studies
- Chromosomal and Genetic Variations
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Resistance
- Cancer-related gene regulation
AgroParisTech
2011-2025
Institut Jean-Pierre Bourgin
2014-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Université Paris-Saclay
2016-2025
Université Libre de Bruxelles
2021
Centre National de la Recherche Scientifique
2007-2019
Université Claude Bernard Lyon 1
2011-2013
Université Paris Cité
2012
Institut National de la Recherche Agronomique
2000-2012
Sorbonne Université
2009-2012
Introduction of transgene DNA may lead to specific degradation RNAs that are homologous the transcribed sequence through phenomena named post-transcriptional gene silencing (PTGS) in plants, quelling fungi, and RNA interference (RNAi) animals. It was shown previously PTGS, quelling, RNAi require a set related proteins (SGS2, QDE-1, EGO-1, respectively). Here we report isolation Arabidopsis mutants impaired PTGS which affected at Argonaute1 ( AGO1 ) locus. is similar QDE-2 required for RDE-1...
Mutants at the PROCUSTE1 (PRC1) locus show decreased cell elongation, specifically in roots and dark-grown hypocotyls. Cell elongation defects are correlated with a cellulose deficiency presence of gapped walls. Map-based cloning PRC1 reveals that it encodes member (CesA6) synthase catalytic subunit family, which least nine other members exist Arabidopsis. Mutations another family member, RSW1 (CesA1), cause similar wall all types, including those hypocotyls roots, suggesting synthesis these...
Post-transcriptional gene silencing (PTGS) is a sequence-specific RNA degradation mechanism that widespread in eukaryotic organisms. It often associated with methylation of the transcribed region silenced and accumulation small RNAs (21 to 25 nucleotides) homologous gene. In plants, PTGS can be triggered locally then spread throughout organism via mobile signal cross graft junction. Previously, we showed helper component–proteinase (HC-Pro) plant potyviruses suppresses PTGS. Here, report...
Epigenetic silencing of transgenes and endogenous genes can occur at the transcriptional level (TGS) or posttranscriptional (PTGS). Because they be induced by viruses, TGS PTGS probably reflect alternative (although not exclusive) responses to two important stress factors that plant's genome has face: stable integration additional DNA into chromosomes extrachromosomal replication a viral genome. TGS, which results from impairment transcription initiation through methylation and/or chromatin...
Abstract Isoxaben is a pre-emergence herbicide that inhibits cellulose biosynthesis in higher plants. Two loci identified by isoxaben-resistant mutants (ixr1-1,ixr1-2, and ixr2-1) Arabidopsis have been reported previously. IXR1 was recently shown to encode the synthase catalytic subunit CESA3 (W.-R. Scheible, R. Eshed, T. Richmond, D. Delmer, C. Somerville [2001] Proc Natl Acad Sci USA 98: 10079–10084). Here, we report on cloning of IXR2, show it encodes another isoform, CESA6. ixr2-1...
Histone acetylation and deacetylation are connected with transcriptional activation silencing in many eukaryotic organisms. Gene families for enzymes that accomplish these modifications show a surprising multiplicity sequence expression levels, suggesting high specificity different targets. We mutations Arabidopsis (Arabidopsis thaliana) HDA6, putative class I histone deacetylase gene, result loss of from several repetitive transgenic endogenous templates. Surprisingly, total levels H4 only...
Abstract The cell expansion phase contributes in determining the major characteristics of a fleshy fruit and represents two-thirds total development tomato (Solanum lycopersicum). So far, it has received very little attention. To evaluate interest genomic scale approach, we performed an initial sequencing approximately 1,200 stage-related sequence tags from at 8, 12, 15 d post anthesis. Interestingly, up to 35% expressed showed no homology with available 21% any known gene. Microarrays...
Despite an ever-increasing interest for the use of pectin-derived oligogalacturonides (OGs) as biological control agents in agriculture, very little information exists-mainly technical reasons-on nature and activity OGs that accumulate during pathogen infection. Here we developed a sensitive OG profiling method, which revealed unsuspected features generated infection Arabidopsis thaliana with fungus Botrytis cinerea Indeed, contrast to previous reports, most were acetyl- methylesterified,...
Small secreted peptides are important players in plant development and stress response. Using a targeted silico approach, we identified family of 14 Arabidopsis genes encoding precursors serine-rich endogenous (PROSCOOP). Transcriptomic analyses revealed that one member this family, PROSCOOP12, is involved processes linked to biotic oxidative as well root growth. Plants defective gene were less susceptible Erwinia amylovora infection showed an enhanced growth phenotype. In PROSCOOP12...
Genes introduced into higher plant genomes can become silent (gene silencing) and/or cause silencing of homologous genes at unlinked sites (homology-dependent gene or HDG silencing). Mutations the HOMOLOGY-DEPENDENT GENE SILENCING1 (HOG1) locus relieve transcriptional and methylation-dependent result in genome-wide demethylation. The hog1 mutant plants also grow slowly have low fertility reduced seed germination. Three independent mutants HOG1 were each found to point mutations 3′ end a...
Abstract Siderophores (ferric ion chelators) are secreted by organisms in response to iron deficiency. The pathogenic enterobacterium Erwinia chrysanthemi produces two siderophores, achromobactin and chrysobactin (CB), which required for systemic dissemination host plants. Previous studies have shown that CB is produced planta can trigger the up-regulation of plant ferritin gene AtFER1. To further investigate function during pathogenesis, we analyzed its effect Arabidopsis (Arabidopsis...
The high affinity nitrate transport system in Arabidopsis thaliana involves one gene and potentially seven genes from the NRT1 NRT2 family, respectively. Among them, NRT2.1, NRT2.2, NRT2.4 NRT2.7 proteins have been shown to are localized on plasmalemma or tonoplast membranes. NRT2.2 play a role uptake soil solution by root cells while is responsible for loading seed vacuole. We undertaken functional characterization of third member NRT2.6 gene. was weakly expressed most plant organs its...
Lytic Polysaccharide Monooxygenases (LPMOs) are powerful redox enzymes able to oxidatively cleave recalcitrant polysaccharides. Widely conserved across biological kingdoms, LPMOs of the AA9 family deployed by phytopathogens deconstruct cellulose polymers. In response, plants have evolved sophisticated mechanisms sense cell wall damage and thus self-triggering Damage Triggered Immunity responses. Here, we show that Arabidopsis exposed LPMO products triggered innate immunity ultimately leading...
Many taxonomically diverse plant species are attacked by Erwinia chrysanthemi, a member of the causal agents soft-rotting diseases. Symptom development is due to collective action pectin-degrading enzymes secreted bacterium through type II secretion system (T2SS). Using Arabidopsis thaliana as susceptible host, we show that plants respond E. chrysanthemi 3937 expressing cell-wall reactions, production an oxidative burst, and activation salicylic acid (SA) jasmonic (JA) or ethylene (ET)...
Eukaryotes have evolved complex defense pathways to combat invading pathogens. Here, we investigated the role of Arabidopsis thaliana heterogeneous nuclear ribonucleoprotein (hnRNP-Q) LIF2 in plant innate immune response. We show that loss-of-function A. leads changes basal expression salicylic acid (SA)- and jasmonic (JA)- dependent marker genes PR1 PDF1.2, respectively. Whereas involved SA JA biosynthesis signaling was also affected lif2-1 mutant, no change hormonal contents detected. In...
Abstract In this study, we investigated the interplay between nitrogen nutrition and pectin degradation dynamics during Arabidopsis Botrytis interaction. Our findings revealed that infected detached leaves from nitrogen-sufficient plants released more lyase (PNL)-derived oligogalacturonides compared to nitrogen-deficient ones. We then focused on BcPNL1, most highly expressed PNL upon infection. Using mutant strains lacking BcPNL1 , observed reduced pathogenicity, a delay in germination lag...
Erwinia amylovora causes fire blight in rosaceous plants. In nonhost Arabidopsis thaliana, E. triggers necrotic symptoms associated with transient bacterial multiplication, suggesting either that A. thaliana lacks a susceptibility factor or it actively restricts growth. Inhibiting plant protein synthesis at the time of infection led to an increase necrosis and multiplication reduced callose deposition, indicating requires active restrict Analysis synthase–deficient pmr4-1 mutant indicated...
Abstract Plant nitrogen (N) fertilization is known to affect disease; however, the underlying mechanisms remain mostly unknown. We investigated impact of N supply on Arabidopsis thaliana–Botrytis cinerea interaction. A. thaliana plants grown in low nitrate were more tolerant all wild‐type B. strains tested. determined leaf concentrations and showed that they had a limited growth vitro. For first time, we performed dual RNA‐Seq infected leaves with different concentrations. Transcriptome...