A5014083734- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and biochemical processes
- Enzyme-mediated dye degradation
- Enzyme Catalysis and Immobilization
- Lignin and Wood Chemistry
- Sugarcane Cultivation and Processing
- Fungal and yeast genetics research
- Microbial Metabolism and Applications
- Microbial Metabolites in Food Biotechnology
- Facial Trauma and Fracture Management
- Ophthalmology and Eye Disorders
- Algal biology and biofuel production
- Plant and animal studies
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Catalysis for Biomass Conversion
- Craniofacial Disorders and Treatments
- Bacterial biofilms and quorum sensing
- Protist diversity and phylogeny
- Marine Sponges and Natural Products
- Fermentation and Sensory Analysis
- Vascular Malformations and Hemangiomas
- Soil Management and Crop Yield
Universidade Estadual de Campinas (UNICAMP)
2013-2024
Universidade de Sorocaba
2017-2024
Université Libre de Bruxelles
2023
Universidade Federal da Paraíba
2021
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2012-2016
Brazilian Center for Research in Energy and Materials
2012-2016
Universidade Federal de Viçosa
2012
Plant cell wall degrading enzymes are key technological components in biomass bioconversion platforms for lignocellulosic materials transformation. Cost effective production of and identification efficient degradation routes two economic bottlenecks that currently limit the use renewable feedstocks through an environmental friendly pathway. The present study describes hypersecretion endo-xylanase (GH11) arabinofuranosidase (GH54) by a fungal expression system with potential biotechnological...
The enzymatic degradation of lignocellulosic materials by fungal enzyme systems has been extensively studied due to its effectiveness in the liberation fermentable sugars for bioethanol production. Recently, variants fungus Penicillium echinulatum have described as a great producer cellulases and considered promising strain industry.Penicillium echinulatum, wild-type 2HH mutant S1M29, were grown on four different carbon sources: cellulose, sugar cane bagasse pretreated steam explosion (SCB),...
Glycoside hydrolases (GHs) and accessory proteins are key components for efficient cost-effective enzymatic hydrolysis of polysaccharides in modern, biochemically based biorefineries. Currently, commercialized GHs produced by ascomycetes. However, the role wood decay basidiomycetes biomass saccharification has not been extensively pursued. Wood fungi degrade highly lignified tissues natural environments, a promising enzyme source improving cocktails that designed vitro lignocellulose...
Este ensaio teórico analisa as “macro e micropolíticas” das lutas urbanas reivindicativas afirmativas na cidade de São Paulo, conforme Suely Rolnik (2018), com ênfase no período redemocratização iniciado nos anos 1980. A análise centra-se relação dessas os processos produção transformação periferias, atualmente ressignificadas como quebradas. Defende-se a hipótese que “periferia” “quebrada” possuem distinções conceituais, embora frequentemente sejam tratados sinônimos. é fundamentada em...
Termites are considered one of the most efficient decomposers lignocelluloses on Earth due to their ability produce, along with its microbial symbionts, a repertoire carbohydrate-active enzymes (CAZymes). Recently, set Pro-oxidant, Antioxidant, and Detoxification (PAD) were also correlated metabolism carbohydrates lignin in termites. The lower termite Coptotermes gestroi is main urban pest Brazil, causing damage wood constructions. analysis enzymatic C. unveiled presence different CAZymes....
Abstract The repertoire of redox-active enzymes produced by the marine fungus Peniophora sp. CBMAI 1063, a laccase hyper-producer strain, was characterized omics analyses. genome revealed 309 Carbohydrate-Active Enzymes (CAZymes) genes, including 48 predicted genes related to modification and degradation lignin, whith 303 being transcribed under cultivation in optimized saline conditions for production. secretome confirmed that can produce versatile ligninolytic enzyme cocktail. It secretes...
Lignin is an attractive alternative for producing biobased chemicals. It the second major component of plant cell wall and abundant natural source aromatic compounds. degradation using microbial oxidative enzymes that depolymerize lignin catabolize compounds into central metabolic intermediates a promising strategy valorization. However, intrinsic heterogeneity recalcitrance severely hinder its biocatalytic conversion. In this context, examining systems can provide fundamental understanding...
Metagenomics has been widely employed for discovery of new enzymes and pathways to conversion lignocellulosic biomass fuels chemicals. In this context, the present study reports isolation, recombinant expression, biochemical structural characterization a novel endoxylanase family GH10 (SCXyl) identified from sugarcane soil metagenome. The SCXyl was highly active against xylan beechwood showed optimal enzyme activity at pH 6,0 45°C. crystal structure solved 2.75 Å resolution, revealing...
Xylanases decrease the xylan content in pretreated biomass releasing it from hemicellulose, thus improving accessibility of cellulose for cellulases. In this work, an endo-β-1,4-xylanase Aspergillus fumigatus var. niveus (AFUMN-GH10) was successfully expressed. The structural analysis and biochemical characterization showed AFUMN-GH10 does not contain a carbohydrate-binding module. enzyme retained its activity pH range 4.5 to 7.0, with optimal temperature at 60 °C. highest beechwood xylan....
Abstract Background Cellulose consisting of arrays linear beta-1,4 linked glucans, is the most abundant carbon-containing polymer present in biomass. Recalcitrance crystalline cellulose towards enzymatic degradation widely reported and result intra- inter-molecular hydrogen bonds within among glucans. Cellobiohydrolases are enzymes that attack cellulose. Here we report on two forms glycosyl hydrolase family 7 cellobiohydrolases common to all Aspergillii Avicel, cotton other Results Cbh1 CelD...
Abstract Background Wood-decay basidiomycetes are effective for the degradation of highly lignified and recalcitrant plant substrates. The lignocellulosic materials by brown-rot strains is carried out carbohydrate-active enzymes non-enzymatic Fenton mechanism. Differences in lignocellulose catabolism among closely related brown rots not completely understood. Here, a multi-omics approach provided global understanding strategies employed L. sulphureus ATCC 52600 degradation. Results genome...
Wood-feeding termites effectively degrade plant biomass through enzymatic degradation. Despite their high efficiencies, however, individual glycoside hydrolases isolated from and symbionts exhibit anomalously low effectiveness in lignocellulose degradation, suggesting hereto unknown activities digestome. Herein, we demonstrate that an ancient redox-active enzyme encoded by the lower termite Coptotermes gestroi, a Cu/Zn superoxide dismutase (CgSOD-1), plays previously role We show CgSOD-1...
One of the current challenges to produce second-generation ethanol is obtain an efficient enzyme complex for hydrolysis lignocellulosic biomass. Genetic improvement lignocellulolytic fungi has helped strains that secrete higher enzymatic titers. This article analyzes profile two Penicillium echinulatum, wild type 2HH and mutant S1M29, grown on different carbon sources (sugarcane bagasse pretreated by steam explosion, cellulose, glucose, glycerol) at cultivation times. To determine...
Glycoside hydrolases (GHs) are involved in the degradation of a wide diversity carbohydrates and present several biotechnological applications. Many GH families composed enzymes with single well-defined specificity. In contrast, from GH16 family can act on range different polysaccharides, including β-glucans galactans. SCLam, member derived soil metagenome, an endo-β-1,3(4)-glucanase (EC 3.2.1.6), cleave both β-1,3 β-1,4 glycosidic bonds glucans, such as laminarin, barley β-glucan,...