A5054508382- Biochemical and biochemical processes
- Lignin and Wood Chemistry
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme-mediated dye degradation
- Catalysis for Biomass Conversion
- Fermentation and Sensory Analysis
- Microbial Metabolism and Applications
- Steroid Chemistry and Biochemistry
- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Biosimilars and Bioanalytical Methods
- Monoclonal and Polyclonal Antibodies Research
University of Warwick
2022-2024
Universidade Estadual de Campinas (UNICAMP)
2019-2023
Universidade de Sorocaba
2019-2022
Lignin is an attractive alternative for producing biobased chemicals. It the second major component of plant cell wall and abundant natural source aromatic compounds. degradation using microbial oxidative enzymes that depolymerize lignin catabolize compounds into central metabolic intermediates a promising strategy valorization. However, intrinsic heterogeneity recalcitrance severely hinder its biocatalytic conversion. In this context, examining systems can provide fundamental understanding...
Abstract A LigE‐type beta‐etherase enzyme from lignin‐degrading Agrobacterium sp. has been identified, which assists degradation of polymeric lignins. Testing against lignin dimer model compounds revealed that it does not catalyse the previously reported reaction Sphingobium SYK‐6 LigE, but instead shows activity for a β‐5 phenylcoumaran dimer. The products did contain glutathione, indicating catalytic role reduced glutathione in this enzyme. Three were identified: major product was cis...
Pathways by which the biopolymer lignin is broken down soil microbes could be used to engineer new biocatalytic routes from renewable chemicals, but are currently not fully understood. In order probe these pathways, we have prepared synthetic lignins containing 13C at sidechain β-carbon. Feeding of [β-13C]-labelled DHP Rhodococcus jostii RHA1 has led incorporation label into metabolites oxalic acid, 4-hydroxyphenylacetic and 4-hydroxy-3-methoxyphenylacetic confirming that they derived...
Abstract To improve the titre of lignin‐derived pyridine‐dicarboxylic acid (PDCA) products in engineered Rhodococcus jostii RHA1 strains, plasmid‐based overexpression seven endogenous and exogenous lignin‐degrading genes was tested. Overexpression multi‐copper oxidases mcoA , mcoB mcoC found to enhance 2,4‐PDCA production by 2.5‐, 1.4‐, 3.5‐fold, respectively, while dye‐decolorizing peroxidase dypB 1.4‐fold, Streptomyces viridosporus laccase enhanced 1.3‐fold. The genomic context R. gene...
Ferulic acid (FA), a low-molecular weight aromatic compound derived from lignin, represents high-value molecule, used for applications in the cosmetic and pharmaceutical industries. FA can be further enzymatically converted other commercially interesting molecules, such as vanillin bioplastics. In several organisms, these transformations often start with common step of activation via CoA-thioesterification, catalyzed by feruloyl-CoA synthetases (Fcs). this context, enzymes are...