A5035398714- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Fungal Biology and Applications
- Enzyme-mediated dye degradation
- Catalysis for Biomass Conversion
- Microbial Metabolites in Food Biotechnology
- Phytochemicals and Antioxidant Activities
- Microbial Metabolism and Applications
- Fungal and yeast genetics research
- Lignin and Wood Chemistry
- Advanced Cellulose Research Studies
- Biochemical and biochemical processes
- Polysaccharides and Plant Cell Walls
- Medicinal Plants and Bioactive Compounds
- Electrochemical sensors and biosensors
- Plant Pathogens and Fungal Diseases
- Protein Hydrolysis and Bioactive Peptides
- Phytochemical compounds biological activities
- Plant-Microbe Interactions and Immunity
- Slime Mold and Myxomycetes Research
- Natural Antidiabetic Agents Studies
- Phytochemistry and Bioactivity Studies
- Chromium effects and bioremediation
Universidade de Caxias do Sul
2016-2025
Pfizer (United Kingdom)
2021
To evaluate the solid-state fermentation (SSF) production of cellulase and hemicellulases (xylanases), by Penicillium echinulatum 9A02S1, in experiments carried out with different concentrations pretreated sugar cane bagasse (PSCB) wheat bran (WB).This study reports xylanolytic cellulolytic enzymes P. 9A02S1 using a cheap medium containing PSCB WB under SSF. The highest amounts filter paper activity (FPA) could be measured on mixtures (32.89 +/- 1.90 U gdm(-1)). beta-glucosidase was 58.95...
The enzymatic degradation of lignocellulosic materials by fungal enzyme systems has been extensively studied due to its effectiveness in the liberation fermentable sugars for bioethanol production. Recently, variants fungus Penicillium echinulatum have described as a great producer cellulases and considered promising strain industry.Penicillium echinulatum, wild-type 2HH mutant S1M29, were grown on four different carbon sources: cellulose, sugar cane bagasse pretreated steam explosion (SCB),...
The performance of two lignocellulosic biomasses was studied in high-pressure carbon dioxide/water pre-treatment. Sugarcane bagasse and elephant grass were used to produce C5-sugars from hemicellulose and, simultaneously, promote cellulose digestibility for enzymatic saccharification. Different pre-treatment conditions, with combined severity factor ranging -1.17 -0.04, evaluated maximal total xylan xylose yields 59.2wt.% (34.4wt.% xylooligomers) 46.4wt.% (34.9wt.% attained sugarcane grass,...
The present work was carried out to determine the best sources and concentrations of carbon nitrogen in production laccases, total peroxidases manganese peroxidase by Marasmiellus palmivorus VE111. Among used, combination glucose casein led higher laccases (5134 U/mL), (187 U/mL) (57 U/mL). Central composite rotatable design (CCRD) used optimum produce enzymes. experiments with better results were scaled up a 5 L bioreactor. It verified that lower (5 10 g/L) stimulated activity (1285 (3420...
In this work, steam explosion was used a pretreatment method to improve the conversion of elephant grass (Pennisetum purpureum) cellulosic ethanol. This way, enzymatic hydrolysis vaccum-drained and water-washed steam-treated substrates carried out with Penicillium echinulatum enzymes while Saccharomyces cerevisiae CAT-1 for fermentation. After 48 h hydrolysis, highest yield reducing sugars obtained from that were produced after 10 min at 200 °C (863.42 ± 62.52 mg/g). However, glucose derived...
Plant feedstocks are at the leading front of biofuel industry based on potential to promote economical, social and environmental development worldwide through sustainable scenarios related energy production. Penicillium echinulatum is a promising strain for bioethanol its capacity produce large amounts cellulases low cost. The secretome profile P. after grown integral sugarcane bagasse, microcrystalline cellulose three types pretreated bagasse was evaluated using shotgun proteomics....
The development of more productive strains microorganisms and processes that increase enzyme levels can contribute to the economically efficient production second generation ethanol. To this end, cellulases xylanases were produced with S1M29 mutant strain Penicillium echinulatum, using different concentrations cellulose (20, 40, 60 g L−1) in batch fed-batch processes. highest activities FPase (8.3 U mL−1), endoglucanases (37.3 (177 mL−1) obtained cultivation 40 L−1 cellulose. P. echinulatum...
There has been a notable increase in interest about cellulases recent years due to their numerous potential applications, including the hydrolysis of cellulose lignocellulosic biomass produce reducing sugars for development second-generation ethanol.However, traditional cellulase assays are tedious, time-consuming and labor intensive.What is more, they require large amounts reagents quantities toxic effluents?The Filter Paper Assay (FPA) standard measure activity, according International...
The combination of hydrothermal pretreatment followed by delignification with imidazole was evaluated for the first time as a potential selective two-stage fractionation elephant grass, aiming at obtaining pure fractions susceptible to conversion high value-added products. In addition, recovery cellulose and hemicelluloses enzymatic hydrolysis yield pretreated grass were evaluated. Hydrothermal 180 °C under non-isothermal conditions allowed liquor rich mainly in xylo- glucooligosaccharides,...
The aim of this study is to improve cellulase production and secretion by Penicillium echinulatum using mutagenesis selection in association with microfermentation microanalysis methods.A new genetic variant was isolated from strain 9A02S1 named S1M29. It obtained H₂O₂ two screening steps, which involved Petri dishes the medium supplemented 2-deoxyglucose microfermentations submerged culture. mutant showed higher productivity than based on Filter Paper Activity assay endoglucanase; peak...