A5018862995- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Polysaccharides and Plant Cell Walls
- Algal biology and biofuel production
- Photodynamic Therapy Research Studies
- Advanced Cellulose Research Studies
- Microbial Metabolites in Food Biotechnology
- Nanoplatforms for cancer theranostics
- Advanced Nanomaterials in Catalysis
- Enzyme-mediated dye degradation
- Biodiesel Production and Applications
- Food composition and properties
Biotech (Brazil)
2024
Universidade de São Paulo
2018-2024
Universidad de Los Andes
2024
Francisco de Paula Santander University
2012
Xylanases decrease the xylan content in pretreated biomass releasing it from hemicellulose, thus improving accessibility of cellulose for cellulases. In this work, an endo-β-1,4-xylanase Aspergillus fumigatus var. niveus (AFUMN-GH10) was successfully expressed. The structural analysis and biochemical characterization showed AFUMN-GH10 does not contain a carbohydrate-binding module. enzyme retained its activity pH range 4.5 to 7.0, with optimal temperature at 60 °C. highest beechwood xylan....
Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that potentiate the deconstruction of polysaccharides through an oxidative mechanism. The catalysis LPMOs is dependent on a reductant to promote divalent copper ion in metalloenzyme its monovalent state. reported molecules can activate organic compounds low molecular weight, enzymes, lignin and lignin-derived compounds, and, recently, photosynthetic pigments. This work reports functional characterization computational...
This work is part of a megaproject that seeks to isolate microalgae the Chlorophyta division native Norte de Santander and identify their potential applications such as lipid production be used biofuel. Here we present isolation 11 strains from found in two different wastewater environments. The collected were cultivated selective media purified through serial dilutions, depletion culture, application penicillin gentamicin. Biomass was evaluated selected: CHL1 (Chlorella sp.) DES1...
The objective of this study was to assess the cellulase production four fungi: Aspergillus terreus NIH2624, clavatus NRRL1, versicolor CBS583.65 and phoenicis ATCC3157, under submerged cultivation conditions. When these fungi were cultured in shake flasks using Mandels Weber’s minimal medium with 1% sugarcane bagasse as a carbon source 1.8 g/L rice bran extract nitrogen source, A. showed maximum (filter paper activity (FPase) 3.35 U/mL; carboxymethyl (CMCase) 1.69 U/mL). Consequently,...
The use of light as abundant, renewable, and clean energy source to boost lytic polysaccharide monooxygenase (LPMO) reactions represents an exciting yet under-explored opportunity. Herein we demonstrated that photosensitizers, commonly used in photodynamic therapy, which act through the photocatalytic Type I mechanism can drive oxidation PASC by LPMOs, whereas II photosensitizers are not capable promoting LPMO activity. We analyzed (methylene blue tetraiodide salt...
LB83 is a versatile biocatalyst that produces plethora of bioactive products thriving on variety feedstocks under the varying culture conditions. In our last study using this microorganism, we found cellulase activity (FPase, 2.27 U/ml; CMCase, 7.42 U/ml) and other plant cell wall degrading enzyme activities grown sugarcane bagasse soybean meal as carbon source nitrogen, respectively. present study, provide insights secretome analysis enzymatic cocktail. The