A5035698185- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Catalysis for Biomass Conversion
- Enzyme-mediated dye degradation
- Microbial Metabolites in Food Biotechnology
- Algal biology and biofuel production
- CRISPR and Genetic Engineering
- Advanced Nanomaterials in Catalysis
- Magnetic and Electromagnetic Effects
- Geomagnetism and Paleomagnetism Studies
- Planetary Science and Exploration
- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Bacterial Genetics and Biotechnology
- Lignin and Wood Chemistry
Universidade de São Paulo
2020-2024
Novo Nordisk Foundation
2022
Technical University of Denmark
2022
Universidade Federal de São Carlos
2022
Cost-efficient plant biomass conversion using biochemical and/or chemical routes is essential for transitioning to sustainable technologies and renewable biofuels. Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that make part of modern hydrolytic cocktails destined degradation. Here, we characterized MtLPMO9A from Thermothelomyces thermophilus M77 (formerly Myceliophthora thermophila) demonstrated it could be efficiently driven by chlorophyllin excited light in the...
Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that potentiate the deconstruction of polysaccharides through an oxidative mechanism. The catalysis LPMOs is dependent on a reductant to promote divalent copper ion in metalloenzyme its monovalent state. reported molecules can activate organic compounds low molecular weight, enzymes, lignin and lignin-derived compounds, and, recently, photosynthetic pigments. This work reports functional characterization computational...
Engineering of bacterial genomes is a fundamental craft in contemporary biotechnology. The ability to precisely edit chromosomes allows for the development cells with specific phenotypes metabolic engineering and creation minimized genomes. Genetic tools are needed select that underwent editing, dual-selection markers enable both positive negative selection highly useful. Here, we present an optimized easy-to-use version
The use of light as abundant, renewable, and clean energy source to boost lytic polysaccharide monooxygenase (LPMO) reactions represents an exciting yet under-explored opportunity. Herein we demonstrated that photosensitizers, commonly used in photodynamic therapy, which act through the photocatalytic Type I mechanism can drive oxidation PASC by LPMOs, whereas II photosensitizers are not capable promoting LPMO activity. We analyzed (methylene blue tetraiodide salt...