A5015306708- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Polysaccharides and Plant Cell Walls
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Seaweed-derived Bioactive Compounds
- Fungal Biology and Applications
- Enzyme Catalysis and Immobilization
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolic Engineering and Bioproduction
- Peptidase Inhibition and Analysis
- Mechanisms of cancer metastasis
- Glycosylation and Glycoproteins Research
- Trypanosoma species research and implications
- Cancer Mechanisms and Therapy
- HIV/AIDS drug development and treatment
- Protein Structure and Dynamics
- Photosynthetic Processes and Mechanisms
- Hemoglobin structure and function
- Venomous Animal Envenomation and Studies
- Phytochemistry and Biological Activities
- Algal biology and biofuel production
- Methane Hydrates and Related Phenomena
- bioluminescence and chemiluminescence research
Brazilian Center for Research in Energy and Materials
2011-2025
Universidade Estadual de Londrina
2015-2019
Brazilian Biosciences National Laboratory
2010-2016
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2010-2015
Brazilian Synchrotron Light Laboratory
2008-2011
Cellulases participate in a number of biological events, such as plant cell wall remodelling, nematode parasitism and microbial carbon uptake. Their ability to depolymerize crystalline cellulose is great biotechnological interest for environmentally compatible production fuels from lignocellulosic biomass. However, industrial use cellulases somewhat limited by both their low catalytic efficiency stability. In the present study, we conducted detailed functional structural characterization...
Cellulases play a key role in enzymatic routes for degradation of plant cell-wall polysaccharides into simple and economically-relevant sugars. However, their low performance on complex substrates reduced stability under industrial conditions remain the main obstacle large-scale production cellulose-derived products biofuels. Thus, this study novel cellulase with unusual catalytic properties from sugarcane soil metagenome (CelE1) was isolated characterized. The polypeptide deduced celE1 gene...
β-1,3–1,4-Glucanases (E.C. 3.2.1.73) hydrolyze linked β-d-glucans, such as lichenan and barley β-glucan. Recombinant β-1,3–1,4-glucanase from Bacillus subtilis expressed in Escherichia coli purified by Ni-NTA chromatography exhibited optimum activity at 50 °C pH 6.0. The catalytic half-life 60 decreased 90 to 5 min when the enzyme was incubated presence absence of Ca2+ respectively. kinetic parameters hydrolysis were 2695, 3.1 1220 for Vmax (μmol/min/mg), Km (mg mL−1) Kcat (s−1), Analysis...
Xanthomonas pathogens attack a variety of economically relevant plants, and their xylan CUT system (carbohydrate utilization with TonB-dependent outer membrane transporter system) contains two major xylanase-related genes, xynA xynB, which influence biofilm formation virulence by molecular mechanisms that are still elusive. Herein, we demonstrated XynA is rare reducing end xylose-releasing exo-oligoxylanase not an endo-β-1,4-xylanase as predicted. Structural analysis revealed insertion in...
Abstract Xylanolytic enzymes from glycoside hydrolase family 43 (GH43) are involved in the breakdown of hemicellulose, second most abundant carbohydrate plants. Here, we kinetically and mechanistically describe non-reducing-end xylose-releasing exo-oligoxylanase activity report crystal structure a native GH43 Michaelis complex with its substrate prior to hydrolysis. Two distinct calcium-stabilized conformations active site xylosyl unit found, suggesting two alternative catalytic routes....
Fatty acid peroxygenases have emerged as promising biocatalysts for hydrocarbon biosynthesis due to their ability perform C-C scission, producing olefins - key building blocks sustainable materials and fuels. These enzymes operate through non-canonical complex mechanisms that yield a bifurcated chemoselectivity between hydroxylation decarboxylation. In this study, we elucidate structural features in P450 decarboxylases enable the catalysis of unsaturated substrates, expanding mechanistic...
The breakdown of cellulose is one the most important reactions in nature1,2 and central to biomass conversion fuels chemicals3. However, microfibrillar organization its complex interactions with other components plant cell wall poses a major challenge for enzymatic conversion4. Here, by mining metagenomic 'dark matter' (unclassified DNA unknown function) microbial community specialized lignocellulose degradation, we discovered metalloenzyme that oxidatively cleaves cellulose. This acts on...
Branching enzymes (BEs) catalyze the formation of branch points in glycogen and amylopectin by cleavage α-1,4 glycosidic bonds subsequent transfer to a new α-1,6 position. BEs generally belong glycoside hydrolase family 13 (GH13); however TK1436, isolated from hyperthermophilic archaeon Thermococcus kodakaraensis KOD1, is first GH57 member, which possesses BE activity. To date, only structure that had been determined GH13-type Escherichia coli. Herein, we have crystal TK1436 native state...
1,3-β-Glucan depolymerizing enzymes have considerable biotechnological applications including biofuel production, feedstock-chemicals and pharmaceuticals. Here we describe a comprehensive functional characterization low-resolution structure of hyperthermophilic laminarinase from Thermotoga petrophila (TpLam). We determine TpLam enzymatic mode operation, which specifically cleaves internal β-1,3-glucosidic bonds. The enzyme most frequently attacks the bond between 3rd 4th residue non-reducing...
Nucleoside diphosphate kinases play a crucial role in the purine-salvage pathway of trypanosomatid protozoa and have been found secretome Leishmania sp., suggesting function related to host-cell integrity for benefit parasite. Due their importance housekeeping functions parasite by prolonging life host cells infection, they become an attractive target drug discovery design. In this work, we describe first structural characterization nucleoside b from parasites (tNDKbs) providing insights...
The breakdown of β-1,4-mannoside linkages in a variety mannan-containing polysaccharides is great importance industrial processes such as kraft pulp delignification, food processing and production second-generation biofuels, which puts premium on studies regarding the prospection engineering β-mannanases. In this work, two-domain β-mannanase from Thermotoga petrophila that encompasses GH5 catalytic domain with C-terminal CBM27 accessory domain, was functionally structurally characterized....
Arabinoxylan is an abundant polysaccharide in industrially relevant biomasses such as sugarcane, corn stover and grasses. However, the arabinofuranosyl di-substitutions that decorate xylan backbone are recalcitrant to most known arabinofuranosidases (Abfs). In this work, we identified a novel GH51 Abf (XacAbf51) forms trimers solution can cope efficiently with both mono- at terminal or internal xylopyranosyl units of arabinoxylan. Using mass spectrometry, kinetic parameters hydrolysis...
Endo-xylanases play a key role in the depolymerization of xylan and recently, they have attracted much attention owing to their potential applications on biofuels paper industries. In this work, we investigated molecular basis for action mode xylanases 10B at high temperatures using biochemical, biophysical crystallographic methods. The crystal structure xylanase from hyperthermophilic bacterium Thermotoga petrophila RKU-1 (TpXyl10B) has been solved native state complex with xylobiose....
Metagenomics has been widely employed for discovery of new enzymes and pathways to conversion lignocellulosic biomass fuels chemicals. In this context, the present study reports isolation, recombinant expression, biochemical structural characterization a novel endoxylanase family GH10 (SCXyl) identified from sugarcane soil metagenome. The SCXyl was highly active against xylan beechwood showed optimal enzyme activity at pH 6,0 45°C. crystal structure solved 2.75 Å resolution, revealing...
Arabinan is a plant structural polysaccharide degraded by two enzymes; alpha-l-arabinofuranosidase and endo-1,5-alpha-l-arabinanase. These enzymes are highly diversified in nature, however, little known about their biochemical biophysical properties. We have characterized novel arabinanase (AbnA) isolated from Thermotoga petrophila with unique thermostable properties such as the insignificant decrease of residual activity after incubation up to 90 degrees C. determined AbnA mode operation...
Phospholipases A(2) (PLA(2)s) are important components of Bothrops snake venoms, that can induce several effects on envenomations such as myotoxicity, inhibition or induction platelet aggregation and edema. It is known venomous non-venomous snakes present PLA(2) inhibitory proteins (PLIs) in their blood plasma. An protein neutralizes the enzymatic toxic activities PLA(2)s from venoms was isolated alternatus plasma by affinity chromatography using immobilized myotoxin BthTX-I CNBr-activated...
β-Xylosidases (EC 3.2.1.37) are among the principal glycosyl hydrolases involved in breakdown of hemicelluloses, catalyzing reduction xylooligosaccharides to free xylose. All GH39 β-xylosidases structurally characterized date display a modular multi-domain organization that assembles tetrameric quaternary structure. In this work, crystal structure and SAXS molecular envelope new β-xylosidase from Caulobacter crescentus (CcXynB2) have been determined. Interestingly, CcXynB2 is monomer...