A5103078391- Enzyme Production and Characterization
- Toxoplasma gondii Research Studies
- Biofuel production and bioconversion
- Biochemical and Structural Characterization
- Microbial Metabolites in Food Biotechnology
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Herpesvirus Infections and Treatments
- Polyomavirus and related diseases
- RNA and protein synthesis mechanisms
- Identification and Quantification in Food
- Legume Nitrogen Fixing Symbiosis
- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- RNA Interference and Gene Delivery
- Plant Molecular Biology Research
- Cytomegalovirus and herpesvirus research
- GABA and Rice Research
- Ubiquitin and proteasome pathways
- Lubricants and Their Additives
- Enzyme Structure and Function
- DNA Repair Mechanisms
- Medicinal Plants and Neuroprotection
- Redox biology and oxidative stress
- Plant Reproductive Biology
The University of Texas at San Antonio
2024
Instituto Tecnológico de Chascomús
2018-2021
Consejo Nacional de Investigaciones Científicas y Técnicas
2010-2021
National University of General San Martín
2018-2021
Laboratório Nacional de Ciência e Tecnologia do Bioetanol
2013-2016
Brazilian Biosciences National Laboratory
2013-2016
Brazilian Center for Research in Energy and Materials
2013-2016
National University of Mar del Plata
2010-2012
Fundación Ciencias Exactas y Naturales
2008-2012
Universidad Autónoma de Madrid
1990-1991
Cellulases play a key role in enzymatic routes for degradation of plant cell-wall polysaccharides into simple and economically-relevant sugars. However, their low performance on complex substrates reduced stability under industrial conditions remain the main obstacle large-scale production cellulose-derived products biofuels. Thus, this study novel cellulase with unusual catalytic properties from sugarcane soil metagenome (CelE1) was isolated characterized. The polypeptide deduced celE1 gene...
Abstract The association of glycosyl hydrolases with catalytically inactive modules is a successful evolutionary strategy that commonly used by biomass-degrading microorganisms to digest plant cell walls. presence accessory domains in these enzymes associated properties such as higher catalytic efficiency, extension the interface and targeting enzyme proper substrate. However, importance linker region synergistic action remains poorly understood. Thus, this study examined how inter-domain...
Toxoplasma gondii is an apicomplexan protozoan parasite with a complex life cycle composed of multiple stages that infect mammals and birds. Tachyzoites rapidly replicate within host cells to produce acute infection during which the disseminates tissues organs. Highly replicative are subject Double Strand Breaks (DSBs) by replication fork collapse ATM, member phosphatidylinositol 3-kinase (PI3K) family, key factor initiates DNA repair activates cell checkpoints. Here we demonstrate treatment...
ABSTRACT Halolysins are subtilisin-like extracellular proteases produced by haloarchaea that possess unique protein domains and salt dependent for structural integrity functionality. In contrast to bacterial subtilases, the maturation mechanism of halolysins has not been addressed. The halolysin Nep is secreted alkaliphilic haloarchaeon Natrialba magadii , recombinant active enzyme synthesized in Haloferax volcanii . contains an N-terminal signal peptide with typical Tat consensus motif (G...
Journal Article Peptide synthesis catalysed by a haloalkaliphilic serine protease from the archaeon Natrialba magadii (Nep) Get access D.M. Ruiz, Ruiz Instituto de Investigaciones Biológicas, Universidad Nacional Mar del Plata – CONICET, Plata, Argentina Search for other works this author on: Oxford Academic Google Scholar N.B. Iannuci, Iannuci Cátedra Microbiología Industrial y Biotecnología, Facultad Farmacia Bioquímica, Buenos Aires, Junín, O. Cascone, Cascone R.E. De Castro Rosana E....
Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon N. that exhibits optimal activity and stability in salt-saturated solutions. In this work, effect of salt on function structure was investigated. absence salt, became unfolded aggregated, leading to loss activity. The enzyme did not recover its structural functional properties even after restoring ideal conditions for catalysis. At concentrations higher than 1 M (NaCl),...
Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon Natrialba magadii. Many proteases have been characterized from archaea to bacteria as adapted hypersaline environments retaining function and stability until 4.0M NaCl. As observed in other halolysins, this can be related presence of C-terminal extension (CTE) sequence. In present work, we compared biochemical properties recombinant protease with truncated form at 134 amino...
The plant-specific class XI myosins (MyoXIs) play key roles at the molecular, cellular and tissue levels, engaging diverse adaptor proteins to transport cargoes along actin filaments. To recognize their cargoes, MyoXIs have a C-terminal globular tail domain (GTD) that is evolutionarily related those of V (MyoVs) from animals fungi. Despite recent advances in understanding functional played by MyoXI plants, structure its GTD, therefore molecular determinants for cargo selectivity recognition,...