The cholinergic system is involved in specific behavioural responses and cognitive processes. Here, we examined potential alterations the brain levels of key enzymes cirrhotic patients animal models with liver failure. An increase (∼30%) activity acetylcholine-hydrolyzing enzyme, acetylcholinesterase (AChE) observed cortex from deceased hepatic coma, while acetylcholine-synthesizing choline acetyltransferase, remains unaffected. In agreement human data, AChE cortical extracts bile duct...

Classical studies of cholinesterase activity during liver dysfunction have focused on butyrylcholinesterase (BuChE), whereas acetylcholinesterase (AChE) has not received much attention. In the current study, and plasma AChE levels were investigated in rats with cirrhosis induced after 3 weeks bile duct ligation (BDL). BDL showed a pronounced decrease (˜50%) compared sham-operated (non-ligated, NL) controls; BuChE appeared unaffected. A selective loss tetrameric (G4) was detected rats, an...

Presenilin 1 (PS1) plays a critical role in the gamma-secretase processing of amyloid precursor protein to generate beta-amyloid peptide, which accumulates plaques pathogenesis Alzheimer's disease (AD). Mutations PS1 cause early onset AD, and proteins that interact with are major functional importance. We report here coimmunoprecipitation acetylcholinesterase (AChE), an enzyme associated plaques. Binding occurs through N-terminal fragment independent peripheral binding site AChE. Subcellular...

Abstract Changes in the glycosylation pattern of brain proteins have been associated with Creutzfeldt–Jakob disease (CJD). We investigated status acetylcholinesterase (AChE) by lectin binding assay. Our data show that lumbar CSF from definite and probable sporadic CJD cases AChE activity is lower compared age‐matched controls. also show, for first time, altered brain. Unlike Alzheimer's disease, which an alteration both levels molecular forms observed, abnormal appears to be unrelated...

Abstract We analyzed whether donepezil differently influences acetylcholinesterase (AChE) variants from cerebrospinal fluid (CSF) in patients with Alzheimer’s disease (AD) after long‐term treatment. Overall CSF‐AChE activity AD before treatment was not different controls, but the ratio between major tetrameric form, G 4 , and smaller 1 2 species significantly lower. AChE levels at study outset were found to correlate positively β‐amyloid 1–42 (Aβ42). When re‐examined 12 months donepezil,...

Chitosan has been considered an environmental-friendly polymer. However, its use in agriculture not extended yet due to relatively low solubility water. N-Methylene phosphonic chitosan (NMPC) is a water-soluble derivative prepared by adding group chitosan. This study demonstrates that NMPC fungicidal effect on the phytopathogenic fungus Fusarium solani f. sp. eumartii (F. eumartii) judged inhibition of F. eumartti mycelial growth and spore germination. affected fungal membrane permeability,...

Potatoes ( Solanum tuberosum L.) are a good source of dietary antioxidant polyphenols. This study investigated the potential antioxidant, bactericidal, and cytotoxic activities in vitro phenolic compounds present tubers one S. spp. (Summerside), three andigena (landraces Moradita, Waicha, Santa María) cultivars. Both content acids, chlorogenic acids (CGAs) being most abundant, activity were higher extracts from skin than flesh. Extracts Moradita flesh Summerside showed bactericidal against...

Andean potatoes (Solanum tuberosum ssp. andigena) are a good source of dietary polyphenols, such as phenolic acid and flavonoids. These polyphenols have several beneficial effects on human health due to their antioxidant properties. Previously, we demonstrated that polyphenol extracts from potato tubers exerted concentration-dependent cytotoxic effect in neuroblastoma cells. However, the mechanisms involved this activity were not explored. Here show Santa María tuber's activated programmed...

According to their sugar recognition specificity, plant lectins are proposed as bioactive proteins with potential in cancer treatment and diagnosis. Helja is a mannose-specific jacalin-like lectin from sunflower which was shown inhibit the growth of certain fungi. Here, we report its recombinant expression prokaryotic system activity neurobalstoma cells. coding sequence fused pET-32 EK/LIC, enterokinase/Ligation-independent cloning vector 35 kDa protein obtained Escherichia coli representing...

Abstract Andean potatoes ( Solanum tuberosum ssp. andigena ) are a good source of dietary polyphenols, such as phenolic acid and flavonoids. These polyphenols have several beneficial effects on human health due to their antioxidant properties. Previously, we demonstrated that polyphenol extracts from potato tubers exerted concentration-dependent cytotoxic effect in neuroblastoma cells. However, the mechanisms involved this activity were not explored. Here show Santa María tuber’s activated...

Abstract Plants produce diverse types of secondary metabolites, including phenolic compounds, which are involved in protection against fungal infections. Sunflower hulls, an under-appreciated by-product the oil industry, is a natural source compounds. The aim this work was to characterize composition extracts from sunflower evaluate their ability inhibit growth phytopathogenic fungi and provide insights on its mechanism action. We obtained extract enriched compounds (EPC) by hydroalcoholic...

In the intensive ongoing study to elucidate relationship between hallmarks of Alzheimer's disease (AD), reciprocal interaction amyloid metabolism and cholinergic enzyme acetylcholinesterase (AChE) has been addressed various levels interactions have suggested. We previously identified presenilin-1 (PS1), active component γ-secretase complex, as an interacting protein AChE. this study, we explored some consequences AChE-PS1 interactions. The expression AChE (also called T or “tailed”) “read...

Mutations in Presenilin 1 (PS1) cause early onset Alzheimer's disease (AD) and interactors of PS1 are major functional importance. To test the hypothesis that may interact with acetylcholinesterase (AChE), an enzyme associated amyloid plaques, we performed co-immunoprecipation experiments using human brain extracts. The binding affinity for different AChE molecular forms was investigated by sedimentation analysis. distribution both proteins also analyzed immunohistochemistry. Finally,...

Reelin is a glycoprotein that essential for the correct cytoarchitectonic organization of developing CNS. Its function in adult brain less understood, although it has been proposed involved signalling pathways linked to neurodegeneration. We have recently reported presence protein cerebrospinal fluid (CSF) and demonstrated elevated levels 180 kDa fragment patients affected by Alzheimer's disease (AD). To confirm these findings, here we analyzed expression brains CSF from AD non–demented...

Abstract Andean potatoes ( Solanum tuberosum L. ssp. andigena ) are a good source of dietary antioxidant polyphenols. We have previously demonstrated that polyphenol extracts from potato tubers exerted dose-dependent cytotoxic effect in human neuroblastoma SH-SY5Y cells, being skin more potent than flesh ones. In order to gain insight into the bioactivities phenolics, we investigated composition and vitro activity total fractions three cultivars (Santa María, Waicha, Moradita). Potato were...

Plants produce diverse types of secondary metabolites, including phenolic compounds, which are involved in protection against fungal infections. Sunflower hulls, an under-appreciated by-product the oil industry, is a natural source compounds. The aim this work was to characterize composition extracts from sunflower evaluate their ability inhibit growth phytopathogenic fungi and provide insights on its mechanism action. We obtained extract enriched compounds (EPC) by hydroalcoholic extraction...