A5079089489- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Protein Hydrolysis and Bioactive Peptides
- Agricultural pest management studies
- Insect Resistance and Genetics
- Transgenic Plants and Applications
- Insect Pest Control Strategies
- Plant-Microbe Interactions and Immunity
- Science and Education Research
- Legume Nitrogen Fixing Symbiosis
- Studies on Chitinases and Chitosanases
- Phytochemistry Medicinal Plant Applications
- Plant Diversity and Evolution
- Insect and Pesticide Research
- Enzyme Production and Characterization
- Pineapple and bromelain studies
- Bee Products Chemical Analysis
- Plant pathogens and resistance mechanisms
- Essential Oils and Antimicrobial Activity
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Phytochemical compounds biological activities
- Seed Germination and Physiology
- Glycosylation and Glycoproteins Research
- Phytase and its Applications
State University of Norte Fluminense
2016-2025
Prefeitura Municipal de Campos dos Goytacazes
2007-2023
Center for Effective Philanthropy
2011
Universidade Estadual de Campinas (UNICAMP)
2004-2007
Universidade Federal de Mato Grosso do Sul
2004-2007
Universidade Federal do Ceará
1996-1998
Universidade Federal de São Paulo
1998
Universidade de Fortaleza
1997
Instituto Politécnico Nacional
1997
Capsicum species are frequently described in terms of genetic divergence, considering morphological, agronomic, and molecular databases.However, descriptions differences based on anatomical characters rare.We examined the anatomy micromorphology vegetative reproductive organs several species.Four accessions representing C. annuum var.annuum, baccatum var.pendulum, chinense, frutescens were cultivated a greenhouse; leaves, fruits seeds sampled their organ structure analyzed by light scanning...
Lipid transfer proteins (LTPs) were thus named because they facilitate the of lipids between membranes in vitro. This study was triggered by characterization a 9‐kDa LTP from Capsicum annuum seeds that we call Ca ‐LTP 1 . repurified, and last chromatographic purification step, propanol used as solvent place acetonitrile to maintain protein's biological activity. Bidimensional electrophoresis band, which corresponds purified , showed presence three isoforms with isoelectric points (pIs) 6.0,...
Thionins are a family of plant antimicrobial peptides (AMPs), which participate in defense system against pathogens. Here we describe some aspects the CaThi thionin-like action mechanism, previously isolated from Capsicum annuum fruits. Thionin-like peptide was submitted to activity assays Candida species for IC50 determination and synergism with fluconazole evaluation. Viability plasma membrane permeabilization assays, induction intracellular ROS production analysis localization yeast cells...
ABSTRACT Plants defend themselves against pathogens with production of antimicrobial peptides (AMPs). Herein we describe the discovery a new antifungal and antibacterial peptide from fruits Capsicum annuum that showed similarity to an already well characterized family plant AMPs, thionins. Other fraction composed two peptides, in which major also Among obtained fractions, 1, is single 7 kDa, was sequenced by Edman method its comparative sequence analysis database (nr) thionin‐like peptides....
In the present study, we identified and characterized two defensin-like peptides in an antifungal fraction obtained from Capsicum chinense pepper fruits inhibited growth of Colletotrichum scovillei, which causes anthracnose. AMPs were extracted pericarp C. peppers subjected to ion exchange, molecular exclusion, reversed-phase a high-performance liquid chromatography system. We investigated endogenous increase reactive oxygen species (ROS), loss mitochondrial functioning, ultrastructure...
Colleters are secretory structures well distributed in many organs of Angiosperms. Ultrastructurally, the colleters cell presents an enhanced endoplasmic reticulum, Golgi apparatus, and mitochondria. Secretion synthesis, transportation, passage through outer wall is poorly characterized. This study characterized anatomy ultrastructure BATHYSA NICHOLSONII (Rubiaceae) evaluated presence protein secretion its antifungal property. Samples were collected prepared according to usual techniques...
Ureases (EC 3.5.1.5) are metalloenzymes that hydrolyze urea into ammonia and CO2. These proteins have insecticidal fungicidal effects not related to their enzymatic activity. The activity of urease is mostly dependent on the release internal peptides after hydrolysis by insect digestive cathepsins. Jaburetox a recombinant version one these peptides, expressed in Escherichia coli. antifungal ureases filamentous fungi occurs at submicromolar doses, with damage cell membranes. Here we evaluated...
Mo-CBP3 is a chitin-binding protein purified from Moringa oleifera Lam. seeds that displays inhibitory activity against phytopathogenic fungi. This study investigated the structural properties and antifungal mode of action this protein. To end, circular dichroism spectroscopy, assays, measurements production reactive oxygen species microscopic analyses were utilized. composed 30.3% α-helices, 16.3% β-sheets, 22.3% turns 30.4% unordered forms. The structure highly stable retains its...
The objective of this study was to isolate antimicrobial peptides from Capsicum baccatum seeds and evaluate their activity inhibitory effects against α-amylase. Initially, proteins the flour C. were extracted in sodium phosphate buffer, pH 5.4, precipitated with ammonium sulfate at 90% saturation. D1 D2 fractions subjected antifungal tests yeasts Saccharomyces cerevisiae, Candida albicans, tropicalis, Kluyveromyces marxiannus, tested α-amylases Callosobruchus maculates human saliva. fraction...