A5102795138- Toxoplasma gondii Research Studies
- Toxin Mechanisms and Immunotoxins
- Trypanosoma species research and implications
- Cytomegalovirus and herpesvirus research
- Heavy Metal Exposure and Toxicity
- Antimicrobial Peptides and Activities
- Transgenic Plants and Applications
- Mercury impact and mitigation studies
- Autophagy in Disease and Therapy
- Environmental Toxicology and Ecotoxicology
- Plant tissue culture and regeneration
- Research on Leishmaniasis Studies
- Synthesis and Characterization of Heterocyclic Compounds
- Parasites and Host Interactions
- Biochemical and Structural Characterization
- Heme Oxygenase-1 and Carbon Monoxide
- Agricultural and Food Sciences
- Agricultural pest management studies
- Parasitic Infections and Diagnostics
- T-cell and Retrovirus Studies
- Bacteriophages and microbial interactions
- Trace Elements in Health
- Rabies epidemiology and control
- Phytochemistry Medicinal Plant Applications
- Soil Management and Crop Yield
State University of Norte Fluminense
2012-2023
Northern State University
2023
GTx (United States)
1997
Universidade Federal do Rio de Janeiro
1992-1996
Fluorescence microscopy, using dyes which specifically label mitochondria, endoplasmic reticulum and the Golgi complex, transmission electron were used to analyze changes occur in organization of these structures during interaction Toxoplasma gondii with host cells. In uninfected cells mitochondria are long filamentous radiate from nuclear region toward cell periphery. After parasite penetration they become shorter tend concentrate around parasite-containing vacuole (parasitophorous vacuole)...
Ivermectin is the drug of choice for many parasitic infections, with more than one billion doses being distributed in onchocerciasis programs. The has been put into focus recently by malaria community because its potential to kill blood-sucking mosquitoes, thereby reducing transmission. However, activity ivermectin against parasite itself only partly investigated. This study aimed investigate vitro asexual and sexual stages Plasmodium falciparum Both late-stage gametocytes were incubated...
Plant defensins are small cysteine-rich peptides and exhibit antimicrobial activity against a variety of both plant human pathogens. Despite the broad inhibitory that different micro-organisms, little is known about their protozoa. In previous study, we isolated defensin named PvD1 from Phaseolus vulgaris (cv. Pérola) seeds, which was seen to be deleterious yeast cells filamentous fungi. It exerted its effects by causing an increase in endogenous production ROS (reactive oxygen species) NO...
Castor cake is a by-product of the extraction oil from seeds castor plants (Ricinus communis). This contains high levels proteins, but toxic protein, ricin, limits its use as an animal feed. Ricin can be efficiently inactivated by treatment with calcium oxide (CaO), which evaluated cytotoxicity assay using LLC-MK2 cells. The mechanism CaO inactivates however, unclear. We report structural changes responsible for ricin inactivation. Purified was treated 0.6% and then analyzed mass...
Fluorescence microscopy, with dyes analog of ceramide, and transmission electron were used to analyze lipid traffic during interaction Toxoplasma gondii host cells. It is C6-NBD-Ceramide (C6-NBD-Cer), a fluorescent stained the Golgi complex where was metabolized into shingolipid glucosylceramide, translocated via plasma membrane living In uninfected cells, C6-NBD-Cer initially concentrated at perinuclear region, after its products present in cytoplasm membrane. infected probe complex. After...
Toxoplasma, which infects all eukaryotic cells, is considered to be a good system for the study of drug action and behavior infected host cells. In present study, we asked if thiosemicarbazone derivatives can effective against tachyzoites morphological ultrastructural features cells parasites are associated with destruction Toxoplasma. The compounds were tested in Vero cell culture using concentration screens (0.1 20 mM). final 1 mM was chosen biological assay. following results obtained: 1)...
Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, component plastic products, fungicide in synthesis biodiesel fuels. After extraction, castor cake with large amount protein obtained. However, this by-product cannot be animal feed presence toxic (ricin) allergenic (2S albumin) proteins. Here, we propose two processes detoxification allergen...
Tachyzoites of Toxoplasma gondii multiplies within the parasitophorous vacuole (PV) host cell. Simultaneously with parasite division growth takes place. Using immunofluorescence microscopy and antibodies recognizing calreticulin, a nonmuscle functional analogue calsequestrin, 76 kDa glycoprotein localized in endoplasmic reticulum (ER), we showed incorporation ER elements cell into containing-T. gondii. In addition enzyme cytochemistry that glucose-6-phosphatase, an marker ER, is also PV....
Melia azedarach (cinnamon) and Azadirachta indica (neem) have a variety of biologically active ingredients against virus, bacteria protozoan parasites; however, little is known about their action on Toxoplasma gondii intracellular development. infects all eukaryotic cells, where it establishes multiplies inside modified vacuole called the parasitophorous until cell ruptures, re-infecting other cells establishing infection. There are no efficient chemotherapies for elimination T. gondii,...
This work aimed to determine the chemical composition of essential oils from rhizomes and leaves Renealmia chrysotricha Petersen, R. breviscapa Poepp.& Endl.and nicolaioides Loes., evaluate biological activities these on three Candida species parasite Trypanosoma cruzi.The were collected in Atlantic Amazon rainforests.Essential isolated characterized by gas chromatography.Βeta-Caryophyllene was found be most predominant compound breviscapa, nicolaioides, whereas (E)-nerolidol abundant...
Abstract The present study examined the testicular structure and sperm morphology of freshwater fish Gymnotus carapo . Testicular were analyzed using light, transmission scanning electron microscopy. Anatomically, testes weighed 0.070 ± 0.01 g oval with a yellow‐white color. G. caparo testis is organized in interstitial tubular compartments. Spermatogenesis occurs seminiferous tubules where germinal epithelium spermatogenic cystis. Germ cells are seen different stages differentiation: type A...
Summary The objective of this study was to characterize the early development and allometric growth grumatã ( Prochilodus vimboides ). We describe a sample 266 eggs larvae obtained through induced spawning. were spherical (mean 3.7 mm diameter), exhibited yellow yolk non-adhesive pelagic after fertilization hydration. time elapsed between cleavage post-flexion stages considered short (328 hours, 8054 hour-degrees) in regard times other Neotropical rheophilic species, but hatching...
Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until lysis host cell. This study was undertaken to evaluate effect hydroxyurea (a specific drug that arrests cell division at G1/S phase) on multiplication T. tachyzoites in infected Vero cells. Infected cells were treated with for periods varying from 5 48 h, and survival morphology parasite determined. Hydroxyurea arrested intracellular all tested. After h incubation hydroxyurea, parasites not easily...