A5024048361- Enzyme Production and Characterization
- Peptidase Inhibition and Analysis
- Protein Hydrolysis and Bioactive Peptides
- Biochemical and Structural Characterization
- Microbial Metabolism and Applications
- Trypanosoma species research and implications
- Venomous Animal Envenomation and Studies
- Enzyme-mediated dye degradation
- Fungal Biology and Applications
- Enzyme Structure and Function
- Nanoparticles: synthesis and applications
- Biochemical and Molecular Research
- Signaling Pathways in Disease
- Biofuel production and bioconversion
- Phytase and its Applications
- Protease and Inhibitor Mechanisms
- Click Chemistry and Applications
- Mosquito-borne diseases and control
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Potato Plant Research
- E-Learning and Knowledge Management
- Covalent Organic Framework Applications
- Coccidia and coccidiosis research
- Phytochemical compounds biological activities
Universidade Federal de São Paulo
2014-2024
Recently, matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) was successfully applied for the detection of carbapenemase activity directly from Gram-negative colonies. Based on this principle, we evaluated performance MALDI-TOF MS rapid positive blood culture vials. A total 100 vials were randomly selected. assay results confirmed by carbapenemase-encoding genes. 110 bacterial isolates recovered. The identified 21 29 (72.4%) carbapenemase-producing...
Breast cancer comprises clinically and molecularly distinct tumor subgroups that differ in cell histology biology show divergent clinical phenotypes impede phase III trials, such as those utilizing cathepsin K inhibitors. Here we correlate the epithelial-mesenchymal-like transition breast cells secretion with activation aggregation of platelets. Cathepsin is up-regulated proteolyze extracellular matrix contributes to invasiveness. Although proteolytically activated receptors (PARs) are by...
Protease production was carried out in solid state fermentation. The enzyme purified through precipitation with ethanol at 72% followed by chromatographies columns of Sephadex G75 and Sephacryl S100. It 80-fold exhibited recovery total activity 0.4%. SDS-PAGE analysis indicated an estimated molecular mass 24.5 kDa the N-terminal sequence first 22 residues APYSGYQCSMQLCLTCALMNCA. Purified protease only inhibited EDTA (96.7%) stimulated Fe(2+) revealing to be a metalloprotease activated iron....
Scytalidoglutamic peptidase (SGP) is the prototype of fungal glutamic peptidases that are characteristically pepstatin insensitive. These enzymes have a unique catalytic dyad comprised Gln53 and Glu136 activate bound water molecule for nucleophilic attack on carbonyl carbon atom scissile peptide bond. The hydrolysis by SGP at bonds with proline in P1′ position rare event among we investigated using series fluorescence resonance energy transfer peptides, Abz-KLXPSKQ-EDDnp, compared...
Snake venom metalloproteinases (SVMPs) belonging to P-I class are able hydrolyze extracellular matrix proteins and coagulation factors triggering local systemic reactions by multiple molecular mechanisms that not fully understood. BmooMPα-I, a SMVP from Bothrops moojeni venom, was active upon neuro- vaso-active peptides including angiotensin I, bradykinin, neurotensin, oxytocin substance P. Interestingly, BmooMPα-I showed strong bias towards hydrolysis after proline residues, which is...
Aspergillus fumigatus is a saprophytic fungus as well so-called opportunist pathogen. Its biochemical potential and enzyme production justify intensive studies about biomolecules secreted by this microorganism. We describe the alkaline serine peptidase production, with optimum activity at 50°C pH of 7.5 reduction in proteolytic presence Al(+3) ions. When using intramolecularly quenched fluorogenic substrates, highest catalytic efficiency was observed amino acid leucine on subsite S'(3)...
The global emergence of coronavirus disease 2019 (COVID-19) has caused substantial human casualties. Clinical manifestations this vary from asymptomatic to lethal, and the symptomatic form can be associated with cytokine storm hyperinflammation. In face urgent demand for effective drugs treat COVID-19, we have searched candidate compounds using in silico approach followed by experimental validation. Here identified celastrol, a pentacyclic triterpene isolated Tripterygium wilfordii Hook F,...
The 3C-like peptidase of the severe acute respiratory syndrome virus (SARS-CoV) is strictly required for viral replication, thus being a potential target development antiviral agents. In contrast to monomeric picornavirus 3C peptidases, SARS-CoV 3CLpro exists in equilibrium between monomer and dimer forms solution, only proteolytically active dilute buffer solutions. this study, increase activity presence kosmotropic salts crowding agents described. activation followed Hofmeister series...
To enable the photoelectrocatalytic treatment of large volumes water containing low concentrations pollutants, this study introduces a hybrid photocatalyst, composed nanotubular oxides grown on TixW alloy (x = 0.5 and 5.0 wt %) modified with UiO-66 MOF, for degradation estrone (E1) 17α-ethinyl estradiol (EE2). The oxide layer (Nt/TixW) was prepared via anodization, while nanoparticles were synthesized by using solvothermal process. Different techniques modifying evaluated to maximize...
The bioprospection for cellulase and protease producers is a promise strategy the discovery of potential biocatalysts use in hydrolysis lignocellulosic materials as well proteic residues. These enzymes can increment turn viable production second generation ethanol from different alternative sources. In this context, goal study was investigation cellulolytic proteolytic abilities bacteria isolated gastrointestinal tract hippopotamus its composting process. It important to highlight that...
Here we report the hydrolytic behavior of recombinant YFV NS2B/NS3 protease against FRET substrates mimicking prime and non-prime region natural polyprotein cleavage sites. While P2-P'1 motif is main factor associated with catalytic efficiency Dengue (DV) West Nile Virus (WNV) protease, show that k(cat)/K(m) varied by more than two orders magnitude, despite presence same in all substrates. The significance this homogeneity - a unique feature among worldwide prominent flavivirus was...
Halophilic microorganisms are able to grow in the presence of salt and also excellent source enzymes biotechnological products, such as exopolysaccharides (EPSs) polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened Organic Composting Production Unit (OCPU) São Paulo Zoological Park Foundation, which processes 4 ton/day organic residues including plant matter from Atlantic Rain Forest, animal manure carcasses mud water treatment. Among microorganisms, eight halotolerant grew at...
Miliin is a serine protease purified from the latex of Euphorbia milii. This work reports effect pH and temperature on catalytic activity miliin, using fluorescence resonance energy transfer (FRET) substrates. displayed highest at 9 35 °C. Subsite mapping shows that subsites S2 to S2′ prefer uncharged residues. The subsite prefers hydrophobic aliphatic amino acids (Val, Pro Ile) defines cleavage site. first one Euphorbiacea proteases. N-terminal sequence showed higher similarity (40%) with...
Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon N. that exhibits optimal activity and stability in salt-saturated solutions. In this work, effect of salt on function structure was investigated. absence salt, became unfolded aggregated, leading to loss activity. The enzyme did not recover its structural functional properties even after restoring ideal conditions for catalysis. At concentrations higher than 1 M (NaCl),...
Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon Natrialba magadii. Many proteases have been characterized from archaea to bacteria as adapted hypersaline environments retaining function and stability until 4.0M NaCl. As observed in other halolysins, this can be related presence of C-terminal extension (CTE) sequence. In present work, we compared biochemical properties recombinant protease with truncated form at 134 amino...