A5033613110- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Polysaccharides and Plant Cell Walls
- Microbial Metabolites in Food Biotechnology
- Fermentation and Sensory Analysis
- Phytase and its Applications
- Polysaccharides Composition and Applications
- Fungal Biology and Applications
- Protein Hydrolysis and Bioactive Peptides
- Fungal and yeast genetics research
- Microbial Metabolism and Applications
- Phytochemicals and Antioxidant Activities
- Food composition and properties
- Plant tissue culture and regeneration
- Ruminant Nutrition and Digestive Physiology
- Enzyme-mediated dye degradation
- Mycotoxins in Agriculture and Food
- Pineapple and bromelain studies
- Biochemical and biochemical processes
- Agricultural and Food Sciences
- Meat and Animal Product Quality
- Chemical synthesis and alkaloids
- Cassava research and cyanide
- Studies on Chitinases and Chitosanases
Universidade Federal da Grande Dourados
2015-2024
Discovery Air (Canada)
2024
Universidade Estadual Paulista (Unesp)
2007-2011
β-Glucosidases catalyze the hydrolysis of cellobiose and cellodextrins, releasing glucose as main product. This enzyme is used in food, pharmaceutical, biofuel industries. The aim this work to improve β-glucosidase production by fungus Lichtheimia ramosa solid-state fermentation (SSF) using various agroindustrial residues evaluate catalytic properties enzyme. A high β-glucosidase, about 274 U/g dry substrate (or 27.4 U/mL), was obtained cultivating on wheat bran with 65% initial moisture, at...
For the first time, production of an extracellular β-glucosidase (Sp-β-gl) by a Sporidiobolus pararoseus yeast strain is reported. The Sp-β-gl activity was quantified, characterized, and assessed for its efficiency in releasing aroma-enhancing compounds wines. maximum enzymatic synthesis after 72 h growth complex media with 20 g/L cellobiose. optimal pH temperature were 5.5 at 50 °C, respectively. It showed wide range stability exhibited quite high thermostability low temperatures. In...
Microbial β-glucosidases can be used in several industrial processes, including production of biofuels, functional foods, juices, and beverages. In the present work, β-glucosidase by solid state cultivation fungus Thermoascus crustaceus a low-cost medium (comprising agroindustrial residues) was evaluated. The highest β-glucosidase, about 415.1 U/g substrate (or 41.51 U/mL), obtained cultivating wheat bran with 70% humidity, during 96 h at 40°C. enzymatic activity optimum pH 4.5 65°C....
Background: Enzyme production by solid state bioprocess (SSB) using residues as substrate for microorganisms is an alternative costs reduction and to avoid their disposal into environment.The aim of this work was evaluate the physiology fungus Lichtheimia ramosa in terms microbial growth amylases, β-glucosidases, carboxymethylcellulase (CMCase), xylanases, via SSB, utilizing wastes Brazilian savannah fruits bocaiuva (Acrocomia aculeata), guavira (Campomanesia pubescens) pequi (Caryocar...
Invertases are used for several purposes; one among these is the production of fructooligosaccharides. The aim this study was to biochemically characterize invertase from industrial Saccharomyces cerevisiae CAT-1 and Rhodotorula mucilaginosa isolated Cerrado soil. optimum pH temperature were 4.0 70 °C 4.5 50 invertase. thermal stability 3.0 10.5 75 R. invertase, respectively. S. 7.0, °C, Both enzymes showed good catalytic activity with 10% ethanol in reaction mixture. hydrolysis by...
Among the enzymes of cellulolytic complex, β-glucosidases are noteworthy due to possibility their application in different industrial processes, such as production biofuels, winemaking, and development functional foods. This study aimed evaluate characterization β-glucosidase from filamentous fungus Gongronella butleri, recently isolated Cerrado soil cultivated agro-industrial residue substrates. The highest β-glucosidase, about 215.4 U/g dry substrate (or 21.5 U/mL), was obtained by...
Protease production was carried out in solid state fermentation. The enzyme purified through precipitation with ethanol at 72% followed by chromatographies columns of Sephadex G75 and Sephacryl S100. It 80-fold exhibited recovery total activity 0.4%. SDS-PAGE analysis indicated an estimated molecular mass 24.5 kDa the N-terminal sequence first 22 residues APYSGYQCSMQLCLTCALMNCA. Purified protease only inhibited EDTA (96.7%) stimulated Fe(2+) revealing to be a metalloprotease activated iron....
An exo-PG obtained from Penicillium viridicatum in submerged fermentation was purified to homogeneity. The apparent molecular weight of the enzyme 92 kDa, optimum pH and temperature for activity were 5 50-55 degrees C. showed a profile an exo-polygalacturonase, releasing galacturonic acid by hydrolysis pectin with high degree esterification (D.E.). Ions Ca(2+) enhanced stability its 30%. K(m) 1.30 absence 1.16 mg mL(-1) presence this ion. In relation V(max) ion increased 1.76 2.07 mumol...
Polygalacturonases are enzymes involved in the degradation of pectic substances, being extensively used food industries, textile processing, degumming plant rough fibres, and treatment wastewaters. Polygalacturonase (PG) production by thermophilic fungus Thermoascus aurantiacus on solid-state fermentation was carried out culture media containing sugar cane bagasse orange proportions 30% 70% (w/w) at 45°C for 4 days. PG obtained purified gel filtration ion-exchange chromatography. The highest...
Peptidases are important because they play a central role in pharmaceutical, food, environmental, and other industrial processes. A serine peptidase from Aspergillus terreus was isolated after two chromatography steps that showed yield of 15.5%. Its molecular mass determined to be 43 kD, by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). This active between pH 5.0 8.0 had maximum activity at 7.0, 45°C. When exposited with 1 M urea, the enzyme maintained 100% used...
Profiling microorganisms with potential for amylase production in low cost culture media has been widely recognized due to its broad applicability. The aim of this study was select yeast strains produce amylolytic enzymes by solid state fermentation. Fifty-four (54) were assessed and three exhibited ability amylases: Candida parapsilosis 14.68 U/mL (146.8 U/g substrate); Rhodotorula mucilaginosa 25.0 (250 substrate), glabrata 25.39 (253.9 fermentation, 120 h at 28°C, using wheat bran 70%...
The present study compared the production and catalytic properties of amylolytic enzymes obtained from fungi Lichtheimia ramosa (mesophilic) Thermoascus aurantiacus (thermophilic). highest amylase in both was observed wheat bran supplemented with nutrient solution (pH 4.0) after 96 hours cultivation, reaching 417.2 U/g dry substrate (or 41.72 U/mL) 144.5 14.45 for L. T. , respectively. showed higher activity at pH 6.0 60°C. amylases produced by were stable between 3.5–10.5 4.5–9.5, 55°C 1...