Roberto da Silva

ORCID: 0000-0003-1468-5752
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About
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Research Areas
  • Enzyme Production and Characterization
  • Biofuel production and bioconversion
  • Enzyme Catalysis and Immobilization
  • Microbial Metabolites in Food Biotechnology
  • Fermentation and Sensory Analysis
  • Polysaccharides and Plant Cell Walls
  • Microbial Metabolic Engineering and Bioproduction
  • Enzyme-mediated dye degradation
  • Phytochemicals and Antioxidant Activities
  • Protein Hydrolysis and Bioactive Peptides
  • Phytase and its Applications
  • Horticultural and Viticultural Research
  • Agricultural and Food Sciences
  • Fungal Biology and Applications
  • Polysaccharides Composition and Applications
  • Microbial Metabolism and Applications
  • Catalysis for Biomass Conversion
  • Food composition and properties
  • Mycorrhizal Fungi and Plant Interactions
  • Biochemical and biochemical processes
  • Pesticide and Herbicide Environmental Studies
  • Sensory Analysis and Statistical Methods
  • Probiotics and Fermented Foods
  • Sugarcane Cultivation and Processing
  • Botanical Research and Applications

Universidade Estadual Paulista (Unesp)
2016-2025

Hispanics in Philanthropy
2024

Centro Universitário Una
2024

Universidade do Estado do Rio de Janeiro
2023

National Institute of Oceanography
2023

Faculdade de Ciências Médicas da Santa Casa de São Paulo
2023

Universidade Federal de Uberlândia
2022

Universidade de São Paulo
1988-2021

Universidade Estadual de Campinas (UNICAMP)
1994-2019

Centro Universitário de Rio Preto
2017

The use of waste as raw material is important for government economy and natural balance. purpose this work was to study the production CMCase xylanase by a Brazilian strain Thermoascus aurantiacus in solid state fermentation (SSF) using different agricultural residues (wheat bran, sugarcane bagasse, orange corncob, green grass, dried sawdust corn straw) substrates without enrichment medium characterize crude enzymes.The extracellular cellulolytic hemicellulolytic enzymes showed that T....

10.1590/s1517-83822005000300006 article EN Brazilian Journal of Microbiology 2005-09-01

The aim of this study was the detailed characterization phenolic composition and determination antioxidant activity Bordô grape (Vitis labrusca) cultivated in South Brazil. edible parts grapes (flesh skin) contained 1130 mg/kg total compounds (as gallic acid), mainly located skins. Anthocyanin content skins high, largely as 3,5-diglucosides (1359 mg/kg, malvidin 3,5-diglucoside). Total flavonols accounted for 154 μmol/kg, with myricetin 3-glucoside principal flavonol both parts. Very low...

10.1021/jf203679n article EN Journal of Agricultural and Food Chemistry 2011-12-05

Aims To obtain new cellulases and xylanases from thermophilic fungi; evaluate their potential for sugarcane bagasse saccharification. Methods Results Thirty-two heat-tolerant fungi were isolated the environment, identified (morphological/molecular tools) production of enzymes was evaluated by solid state fermentation using lignocellulosic materials as substrates. Myceliophthora thermophila JCP 1-4 best producer endoglucanase (357·51 U g−1), β-glucosidase (45·42 xylanase (931·11 g−1)...

10.1111/jam.12757 article EN Journal of Applied Microbiology 2015-01-21

One hundred sixty eight bacterial strains, isolated from soil and samples of vegetable in decomposition, were screened for the use citrus pectin as sole carbon source. 102 positive pectinase depolymerization assay plates evidenced by clear hydrolization halos. Among them, 30% presented considerable pectinolytic activity. The cultivation these strains submerged semi-solid fermentation polygalacturonase production indicated that five Bacillus sp produced high quantities enzyme....

10.1590/s0001-37141999000400002 article EN Revista de Microbiologia 1999-12-01

Pectin lyase and polygalacturonase production by newly isolated Penicillium viridicatum strain Rfc3 was carried out means of solid state fermentation using orange bagasse, corn tegument, wheat bran mango banana peels as carbon sources. The maximal activity value (Pg) (30U.g-1) obtained source while pectin (Pl) (2000 U.g-1) in medium composed bagasse. Mixtures or with sugar cane bagasse resulted increased Pg Pl compared to fermentations which this residue not used. mixture (50%) the 55 U.g-1...

10.1590/s1517-83822002000400008 article EN Brazilian Journal of Microbiology 2002-12-01

Pectin lyase and polygalacturonase production by newly isolated fungal strains was carried out in solid-state fermentation. Moniliella SB9 Penicillium sp EGC5 produced polygalcturonase (PG) pectin (PL) on mixture of orange bagasse, sugar cane bagasse wheat bran as substrate. PG PL presented optimum activity at pH 4.5 10.0 55 45°C, respectively, while these enzymes from 4.5-5.0 9.0 40°C, respectively.

10.1590/s1516-89132004000500018 article EN cc-by-nc Brazilian Archives of Biology and Technology 2004-09-01

Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter sugarcane piles based on their ability to grow at 45ºC medium containing corn straw cardboard as carbon sources. These fungi identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor Candida. The majority of produced xylanase cellulases under solid state fermentation (SSF). highest cellulase productions obtained by cultivation Aspergillus fumigatus M.7.1...

10.1590/s1517-83822012000300032 article EN Brazilian Journal of Microbiology 2012-09-01

Microbial rennet-like milk-clotting enzymes are aspartic proteinases that catalyze milk coagulation, substituting calf rennet. Crude enzymatic extract produced by the thermophilic fungus, Thermomucor indicae-seudaticae N31, on solid state fermentation (SSF) using wheat bran, exhibited high activity and low proteolytic after 24 h of fermentation. Highest (MCA) was at pH 5.7, 70 °C in 0.04 M CaCl2; it stable range 3.5–4.5 for up to 45 1 h. MCA highly inhibited pepstatin A. Hydrolytic profile...

10.1016/j.foodchem.2009.09.075 article EN publisher-specific-oa Food Chemistry 2009-09-27

Sugarcane bagasse (SCB) was treated in three stages using ozone oxidation (O), washing an alkaline medium (B) and ultrasonic irradiation (U). The impact of each pretreatment stage on the physical structure SCB evaluated by its chemical composition, infrared technique (FTIR-ATR), thermogravimetric analysis (TGA/DTG). sequence O, B, U provided a significant reduction lignin hemicellulose, which confirmed changes absorption bands corresponding to these compounds, when observed infrared....

10.1016/j.biortech.2016.06.072 article EN publisher-specific-oa Bioresource Technology 2016-06-25
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