A5011066877- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Essential Oils and Antimicrobial Activity
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Banana Cultivation and Research
- Phytoplasmas and Hemiptera pathogens
- Research on Leishmaniasis Studies
- Bacterial Genetics and Biotechnology
- Enzyme-mediated dye degradation
- Microbial Metabolites in Food Biotechnology
- Growth and nutrition in plants
- Insect symbiosis and bacterial influences
- Bioactive Compounds and Antitumor Agents
- Curcumin's Biomedical Applications
- Nanocomposite Films for Food Packaging
- Pineapple and bromelain studies
- Cassava research and cyanide
- Trypanosoma species research and implications
- Antimicrobial agents and applications
- Plant Virus Research Studies
- Tannin, Tannase and Anticancer Activities
Universidade Estadual Paulista (Unesp)
2016-2025
Universidade de São Paulo
2016-2025
University of Aveiro
2024
Universidade Paranaense
2024
Universidade Paulista
2024
Universidade Federal de Pernambuco
2019
Centro Universitário da Cidade
2019
University of Pennsylvania
2019
Fundação de Apoio à Universidade Federal de São Paulo
2010
University of Oxford
2001-2003
Xanthomonas citri (Xac) is the causal agent of citrus canker, a disease that affects crops and causes economic impact worldwide. To further characterize cell division in this plant pathogen, we investigated role protein MinC division, chromosome segregation, peptidoglycan incorporation by deleting gene minC using allele exchange. Xac with deleted exhibited classic Δmin phenotype observed other bacteria for min components: minicells short filamentation. In addition noticed formation branches,...
Xanthomonas citri subsp. (Xcc) causes citrus canker, affecting sweet orange-producing areas around the world. The current chemical treatment available for this disease is based on cupric compounds. For reason, objective of study was to design antibacterial agents. In order do this, we analyzed anti-Xcc activity 36 alkyl dihydroxybenzoates and found 14 active Among them, three esters with lowest minimum inhibitory concentration values were selected; compounds 4 (52 μM), 16 (80 μM) 28 (88 μM)....
Abstract Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used nanoparticulate materials due to their antimicrobial properties, but main mechanism action (MOA) has not been fully elucidated. This study characterized ZnO NPs by using X-ray diffraction, FT-IR spectroscopy and scanning electron microscopy. Antimicrobial activity against clinically relevant bacteria Escherichia coli , Staphylococcus aureus Pseudomonas aeruginosa Gram-positive model Bacillus subtilis was evaluated...
Summary Regulation of chromosome inheritance is essential to ensure proper transmission genetic information. To accomplish accurate genome segregation, cells organize their chromosomes and actively separate them prior cytokinesis. In Bacillus subtilis the Spo0J protein required for segregation it regulates developmental switch from vegetative growth sporulation. a DNA‐binding that recognizes at least eight identified parS sites located near origin replication. As judged by fluorescence...
ABSTRACT The plant-pathogenic bacterium Xanthomonas citri subsp. is the causal agent of Asiatic citrus canker, a serious disease that affects all cultivars in subtropical citrus-producing areas worldwide. There no curative treatment for canker; thus, eradication infected plants constitutes only effective control spread X. . Since program state São Paulo, Brazil, under threat, there clear risk becoming endemic main orange-producing area world. Here we evaluated potential use alkyl gallates to...
Alkyl gallates are compounds with reported antibacterial activity. One of the modes action is binding alkyl to bacterial membrane and interference integrity. However, also cause cell elongation disruption division in important plant pathogen Xanthomonas citri subsp. citri, suggesting that proteins may be targeted by gallates. Here, we use Bacillus subtilis purified B. FtsZ demonstrate a direct target disrupt FtsZ-ring vivo, elongation. In vitro, bind high affinity FtsZ, causing it cluster...
Isobavachalcone (IBC) is a natural prenylated chalcone with broad spectrum of pharmacological properties. In this work, we newly synthesized and investigated the antibacterial activity IBC against Gram-positive, Gram-negative mycobacterial species. was active Gram-positive bacteria, mainly Methicillin-Susceptible Staphylococcus aureus (MSSA) Methicillin-Resistant (MRSA), minimum inhibitory concentration (MIC) values 1.56 3.12 µg/mL, respectively. On other hand, not able to act species (MIC >...
The São Paulo state citrus belt in Brazil is a major production region. Since at least 1957, plantations this region have been affected by canker, an economically damaging disease caused Xanthomonas citri subsp. (Xcc). For about 50 years, until 2017, canker eradication programme was carried out In work, our aim to investigate the effects of on genetic variability and evolution Xcc. To end, we sequenced analysed 758 Xcc genomes sampled belt, together with 730 publicly available from around...
Abstract This research investigated active biomolecules from the endophytic fungus Aspergillus unguis (CBMAI 2140), isolated Passiflora incarnata leaves, as potential antibacterial agents against Xanthomonas citri subsp. citri, causative agent of citrus canker. The fungal extract was obtained via liquid-liquid extraction with ethyl acetate. Antibacterial activity assessed using Resazurin Microtiter Assay Plate (REMA), determining inhibitory concentrations (IC90) and Minimum Bactericidal...
Abstract BACKGROUND The rise of herbicide‐resistant weeds like Conyza canadensis L. poses a challenge to modern agriculture, driving the need for eco‐friendly alternatives. Microbial metabolites from actinobacteria species offer promising weed‐control solutions. This study aims screen and identify an isolate Brazil's Caatinga biome that produces phytotoxic characterize its compounds. RESULTS An isolate, named as Caat 7–52, was selected because significant effects against Lemna minor...
Este estudo teve como objetivo comparar a cinética de desnaturação térmica das proteínas presentes no leite búfala e vaca, por meio análises espectroscópicas em UV-visível, com comprimento onda ajustado 500 nm. As foram desnaturadas alcalino, utilizando solução hidróxido sódio, concentração amostras foi determinada base na Lei Lambert-Beer. Os dados experimentais utilizados para aplicar modelos cinéticos primeira, segunda terceira ordem, sendo primeira ordem aquela que apresentou melhor...
Summary Microbiome surveys provide clues for the functional roles of symbiotic microbial communities and their hosts. In this study, we elucidated bacterial microbiomes associated with vertically transmitted fungal inocula (pellets) used by foundress queens leaf‐cutting ant Atta texana as starter‐cultures new gardens. As reference microbiomes, also surveyed queens, gardens brood incipient nests. Pseudomonas , Acinetobacter Propionibacterium Corynebacterium were consistently present in high...
A dendronized perylene bisimide (PBI) that self-organizes into hexagonal arrays of supramolecular double helices with identical single-crystal-like order disregards chirality was recently reported. cogwheel model self-assembly explains this process proposed. Accessing the highly ordered phase required very slow heating and cooling or extended periods annealing. Analogous PBIs linear alkyl chains did not exhibit assembly. Here a library sequence-defined dendrons containing all possible...
Plant diseases caused by phytopathogens are responsible for significant crop losses worldwide. Resistance induction and biological control have been exploited in agriculture due to their enormous potential. In this study, we investigated the antimicrobial potential of endophytic fungi leaves petioles medicinal plants Vochysia divergens Stryphnodendron adstringens located two regions high diversity Brazil, Pantanal, Cerrado, respectively. We recovered 1,304 fungal isolates based on...
Abstract The development of efficient and environmentally friendly plant protection systems is one the major challenges for a sustainable agriculture future. Citrus canker caused by pathogen Xanthomonas citri subsp. ( X . ) affects all cultivated citrus species worldwide responsible enormous economic losses restrictions in international trade. Currently used commercial copper‐based formulations are origin contamination soil groundwater, affecting local ecosystems human health. A copper‐free...
Curcumin is the main constituent of turmeric, a seasoning popularized around world with Indian cuisine. Among benefits attributed to curcumin are anti-inflammatory, antimicrobial, antitumoral, and chemopreventive effects. Besides, inhibits growth gram-positive bacterium Bacillus subtilis. The anti-B. subtilis action happens by interference division protein FtsZ, an ancestral tubulin widespread in Bacteria. FtsZ forms protofilaments GTP-dependent manner, concomitant recruitment essential...
Xanthomonas citri ssp. (Xac) is the causal agent of citrus canker, an economically important disease that affects worldwide. To initiate characterization essential biological processes Xac, we constructed integrative plasmids for ectopic expression green fluorescent protein (GFP)-labeled proteins within this bacterium. Here, show disruption α-amylase gene (amy), site plasmid integration into bacterial chromosome, does not alter its pathogenesis while abolishing completely ability Xac to...
Plant Essential Oils and their constituents are well-known for properties as antimicrobial agents labeled Generally Recognized Safe (GRAS), prompting studies around usage in the control of food-borne microorganisms phytopathogens. In this study, we evaluated Oregano Oil (OEO), Thymol (THY) Carvacrol (CAR) Xanthomonas citri subsp. ( X. ). vitro antibacterial assays revealed that CAR THY inhibit growth at concentrations 100 µg.mL -1 114 , lower than OEO (136 Bactericidal effects were observed...