A5015972947- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Pharmacogenetics and Drug Metabolism
- Innovative Microfluidic and Catalytic Techniques Innovation
- Chemical Synthesis and Analysis
- Enzyme Production and Characterization
- Electrochemical sensors and biosensors
- Biofuel production and bioconversion
- Advanced biosensing and bioanalysis techniques
- biodegradable polymer synthesis and properties
- Protein purification and stability
- Biochemical and Structural Characterization
- Enzyme Structure and Function
- Click Chemistry and Applications
- Photosynthetic Processes and Mechanisms
- Protein Structure and Dynamics
- Analytical Chemistry and Chromatography
- Polymer Surface Interaction Studies
- Metal-Catalyzed Oxygenation Mechanisms
- Computational Drug Discovery Methods
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Enzyme-mediated dye degradation
RWTH Aachen University
2016-2025
DWI – Leibniz Institute for Interactive Materials
2016-2025
Czech Academy of Sciences, Institute of Biotechnology
2014-2024
Forschungszentrum Jülich
2021-2022
FH Aachen
2014-2021
Norwegian Institute of Bioeconomy Research
2019-2021
Beijing University of Chemical Technology
2021
Tsinghua University
2021
Nanjing Tech University
2021
Institute of Process Engineering
2021
Cytochrome p450 BM-3 (EC 1.14.14.1) catalyzes the hydroxylation and/or epoxidation of a broad range substrates, including alkanes, alkenes, alcohols, fatty acids, amides, polyaromatic hydrocarbons, and heterocycles. For many these notoriously water-insoluble compounds, BM-3's K(m) values are in millimolar range. Polar organic cosolvents therefore added to increase substrate solubility achieve high catalytic efficiency. Using as catalyst for important transformations requires that we improve...
Cellulases are promising catalysts for the depolymerization of cellulose under mild conditions. Reengineered cellulases required to match application demands in biorefineries and avoid cost-intensive downstream processing. This manuscript provides a novel fluorescence-based high throughput screening method directed evolution cellulases, based on 4-methylumbelliferyl-β-D-cellobioside (4-MUC). The 4-MUC system was successfully employed identify CelA2 variants with enhanced stability activity...
The asymmetric reduction of ketones is performed by using lyophilized whole cells in neat substrates with defined water activity (aw). Ketones and alcohols prone to be unstable aqueous media can now converted via biocatalysis.
Diaryl ketones are important building blocks for synthesizing pharmaceuticals and generally regarded as "difficult-to-reduce" due to the large steric hindrance of their two bulky aromatic side chains. Alcohol dehydrogenase from Kluyveromyces polyspora (KpADH) has been identified a robust biocatalyst its high conversion diaryl ketone substrate (4-chlorophenyl)(pyridine-2-yl)ketone (CPMK) with moderate R-selectivity 82% ee. To modulate stereoselectivity KpADH, "polarity scanning" strategy was...
Abstract Foliar application of micronutrients (e.g. Fe 3+ ) onto plants over an extended time is challenging and often not possible due to insufficient rainfastness. Smart delivery systems which enable micronutrient release several weeks would offer innovative sustainable options improve plant health food production. Herein, we report a novel foliar fertilizer system based on functional pH‐responsive biohybrid microgels that have orthogonal functionality as carriers employ peptides (termed...
Accumulation of microplastic in the environment and food chain will be a grand challenge for our society. Polyurethanes are widely used synthetic polymers medical (e.g. catheters) industrial products (especially as foams). Polyurethane is not abundant nature only few microbial strains (fungi bacteria) enzymes (polyurethaneases cutinases) have been reported to efficiently degrade polyurethane. Notably, long period time (from 50 >100 years depending on literature) required degradation...
In recent years, the engineering of flexible loops to improve enzyme properties has gained attention in biocatalysis. Herein, we report a loop strategy stability substrate access tunnels, which reveals molecular mechanism between and tunnels. Based on dynamic tunnel analysis CYP116B3, five positions (A86, T91, M108, A109, T111) B-B′ B′-C potentially affecting frequent occurrence were selected subjected simultaneous saturation mutagenesis. The best variant 8G8 (A86T/T91L/M108N/A109M/T111A)...
Global polymer production is about to exceed 400 megatons yearly, and recycling methods enabling a climate-neutral, circular economy are highly important successfully address global challenges like environmental pollution climate change. Polyamides (nylon-6, nylon-6,6) represent the third most-produced hydrolyzable polymer, behind polyurethanes polyethylene terephthalate. The main in developing enzymatic polyamide processes limited number of reported polyamidases lack screening systems that...
Abstract Continuous-flow biocatalysis utilizing immobilized enzymes emerged as a sustainable route for chemical synthesis. However, inadequate biocatalytic efficiency from current flow reactors, caused by non-productive enzyme immobilization or enzyme-carrier mismatches in size, hampers its widespread application. Here, we demonstrate general-applicable and robust approach the fabrication of high-performance enzymatic continuous-flow reactor via integrating well-designed scalable isoporous...
The self-sufficient cytochrome P450 BM-3 enzyme from Bacillus megaterium catalyzes subterminal hydroxylation of saturated long-chain fatty acids and structurally related compounds. Since the primary structure is homologous to that mammalian type II, it represents an excellent model for this family enzymes. During studies on directed evolution into a medium-chain fatty-acid hydroxylase, several mutants, in particular triple mutant Phe87Val, Leu188Gln, Ala74Gly, were observed hydroxylate...
Cytochrome P450 monooxygenase BM-3 (EC 1.14.14.1) hydroxylates fatty acids with chain lengths between C12 and C18. It is also known to oxidize the corresponding alcohols amides. However, it not alkanes. Here we report that oxidizes octane, which four carbons shorter lacks carboxylate functionality of shortest acid accept, 4-octanol, 3-octanol, 2-octanol, 4-octanone, 3-octanone. The rate much lower than for oxidation preferred substrates. In an effort explore plasticity mechanisms substrate...