A5020594243- Polymer Surface Interaction Studies
- Advanced Polymer Synthesis and Characterization
- Advanced Biosensing Techniques and Applications
- Nanofabrication and Lithography Techniques
- Bacterial biofilms and quorum sensing
- Advanced biosensing and bioanalysis techniques
- Graphene and Nanomaterials Applications
- Surface Modification and Superhydrophobicity
- Bacteriophages and microbial interactions
- Membrane Separation Technologies
- Antimicrobial Peptides and Activities
- Electrospun Nanofibers in Biomedical Applications
- Microplastics and Plastic Pollution
- Click Chemistry and Applications
- Molecular Junctions and Nanostructures
- Mycotoxins in Agriculture and Food
- biodegradable polymer synthesis and properties
- Hydrogels: synthesis, properties, applications
- Biosensors and Analytical Detection
- Transgenic Plants and Applications
- Cancer Research and Treatments
- Antimicrobial agents and applications
- Infection Control in Healthcare
- Cellular Mechanics and Interactions
- Block Copolymer Self-Assembly
DWI – Leibniz Institute for Interactive Materials
2016-2024
RWTH Aachen University
2016-2022
Czech Academy of Sciences, Institute of Macromolecular Chemistry
2015-2016
FH Aachen
2016
Czech Academy of Sciences
2015
Global polymer production is about to exceed 400 megatons yearly, and recycling methods enabling a climate-neutral, circular economy are highly important successfully address global challenges like environmental pollution climate change. Polyamides (nylon-6, nylon-6,6) represent the third most-produced hydrolyzable polymer, behind polyurethanes polyethylene terephthalate. The main in developing enzymatic polyamide processes limited number of reported polyamidases lack screening systems that...
Implantable sensor devices require coatings that efficiently interface with the tissue environment to mediate biochemical analysis. In this regard, bioinspired polymer hydrogels offer an attractive and abundant source of coating materials. However, upon implantation these materials generally elicit inflammation foreign body reaction as a consequence protein fouling on their surface concomitant poor hemocompatibility. report we investigate strategy endow chitosan hydrogel antifouling...
Surface-initiated photoinduced SET-LRP of <italic>N</italic>-(2-hydroxypropyl)methacrylamide from an initiator-containing monolayer yields micropatterned brushes resistant to blood plasma fouling.
Abstract Nonthrombogenic modifications of membranes for extracorporeal membrane oxygenators (ECMOs) are key interest. The absence hemocompatibility these and the need anticoagulation patients result in severe potentially life‐threatening complications during ECMO treatment. To address lack membrane, surface developed, which act as barriers to protein adsorption on and, this way, prevent activation coagulation cascade. based nonionic zwitterionic polymer brushes grafted directly from...
Surface‐initiated photo‐induced copper‐mediated radical polymerization is employed to graft a wide range of polyacrylate brushes from silicon substrates at extremely low catalyst concentrations. This the first time that controlled nature reported process demonstrated via block copolymer formation and re‐initiation experiments. In addition unmatched copper concentrations in few ppb, film thicknesses up almost 1 μm are achieved within only h. image
A compact optical biosensor for direct detection of thrombin in human blood plasma (HBP) is reported. This platform based on wavelength spectroscopy diffraction-coupled surface plasmons a chip with periodically corrugated gold film that carries an antifouling thin polymer layer consisting poly[(N-(2-hydroxypropyl)methacrylamide)-co-(carboxybetaine methacrylamide)] (poly(HPMA-co-CBMAA)) brushes. architecture provides superior resistance to nonspecific and irreversible adsorption abundant...
Abstract Wound infections originate when exogenous or endogenous bacterial pathogens can circumvent the barrier of wound dressing and invade bed. Bacterial colonization causes inflammation, stalls healing process, carries risk dissemination to other tissues. In addition, current antimicrobial dressings fail resolve an infection once it has been established because debris killed bacteria rapidly accumulates on their surface hampers action. Faced with this challenge, hybrid synthetic‐natural...
The growth of polymer brushes from a variety methacrylate monomers was accomplished using UV light as polymerization trigger.
Interfacing artificial materials with biological tissues remains a challenge. The direct contact of their surface the milieu results in multiscale interactions, which biomacromolecules adsorb and act as transducers mediating interactions cells tissues. So far, only antifouling polymer brushes have been able to conceal synthetic materials. However, complex synthesis has precluded translation applications. Here, it is shown that ultrathin surface-attached hydrogel coatings N-(2-hydroxypropyl)...
The integration of active cell machinery with synthetic building blocks is the bridge toward developing cells biological functions and beyond. Self-replication one most important tasks living systems, various complex machineries exist to execute it. In Escherichia coli, a contractile division ring positioned mid-cell by concentration oscillations self-organizing proteins (MinCDE), where it severs membrane wall. So far, reconstitution any has exclusively been tied liposomes. Here, rudimentary...
Currently, one of the most promising treatments lipopolysaccharides (LPS)-induced sepsis is based on hemofiltration. Nevertheless, proteins rapidly adsorbed artificial surface membranes which leads to activation coagulation impairing effective scavenging endotoxins. To overcome this challenge, we designed polymer-brush-coated microparticles displaying antifouling properties and functionalized them with polymyxin B (PMB) specifically scavenge LPS common endotoxin. Poly[(N-(2-hydroxypropyl)...
Macrophages will play an important role in future diagnostics and immunotherapies of cancer. However, this demands to selectively capture sort different subpopulations, which remains a challenge due their innate ability bind wide range interfaces indiscriminately. The main obstacle here is the lack combining sufficient antifouling properties with display specific binding sites allowing sorting quantification. Herein, as proof principle means, it introduced pattern locally selective...
Abstract Strain‐promoted alkyne‐azide cycloaddition (SPAAC) has become an indispensable tool in bioorthogonal conjugation and surface immobilization. While numerous studies have focused on enhancing the reactivity of cyclooctynes, a facile method to evaluate binding efficiency for cyclooctyne‐azide‐based immobilization without any sophisticated facilities is still missing. In present work, different derivatives dibenzocyclooctyne/bicyclononyne (DBCO/BCN) linked either fluorophore or...
Buffers/solutions suitable for total desorption of adsorbed proteins on low fouling polymer brushes are presented, enabling analysis not only by MS, but also a plethora other state-of-the-art proteomics methods.
Abstract Management of synthetic polymer waste is one the most pressing challenges for society today. Enzymatic recycling polycondensates like polyamides (PA), however, remains limited due to a lack efficient polyamidases. This study reports first directed evolution campaign polyamidase, NylC p2 -TS. Key positions involved in enzyme-substrate interactions and PA 6 hydrolysis are identified through random mutagenesis molecular dynamics (MD) simulations. The final variant, NylC-HP (NylC -TS...
Antifouling Polymer Brushes In article number 2102325, Bingquan Yang, Michael Hirtz, and co-workers investigate the efficiency of cyclooctyne click chemistry based patterned immobilization functional compounds on azide-bearing diblock polymer brushes. Microchannel cantilever spotting (μCS) enables microarrays antifouling brushes that can act as selective adhesion spots for proteins favorable anti-adhesion properties polymer.