A5020329666- Polymer Surface Interaction Studies
- Nanofabrication and Lithography Techniques
- Advanced Polymer Synthesis and Characterization
- Advanced biosensing and bioanalysis techniques
- Molecular Junctions and Nanostructures
- Lipid Membrane Structure and Behavior
- Dendrimers and Hyperbranched Polymers
- Electrospun Nanofibers in Biomedical Applications
- Bacterial biofilms and quorum sensing
- Surface Modification and Superhydrophobicity
- Marine Biology and Environmental Chemistry
- Advanced Biosensing Techniques and Applications
- 3D Printing in Biomedical Research
- Supramolecular Self-Assembly in Materials
- Bone Tissue Engineering Materials
- RNA Interference and Gene Delivery
- biodegradable polymer synthesis and properties
- Antimicrobial agents and applications
- Hydrogels: synthesis, properties, applications
- Nanoparticle-Based Drug Delivery
- Innovative Microfluidic and Catalytic Techniques Innovation
- Blood properties and coagulation
- Antimicrobial Peptides and Activities
- Hemostasis and retained surgical items
- Microfluidic and Bio-sensing Technologies
Instituto de Salud Carlos III
2023-2025
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2023-2025
Institute for Bioengineering of Catalonia
2022-2025
Institució Catalana de Recerca i Estudis Avançats
2022-2025
DWI – Leibniz Institute for Interactive Materials
2016-2025
RWTH Aachen University
2016-2023
FH Aachen
2016-2020
Czech Academy of Sciences, Institute of Macromolecular Chemistry
2009-2017
Czech Academy of Sciences
2013-2016
Institute of Macromolecular Chemistry
2014-2016
Nonspecific adsorption of proteins is a crucial problem in the detection analytes complex biological media by affinity sensors operating with label-free detection. We modified gold surface plasmon resonance (SPR) three types promising antifouling coatings: self-assembled monolayers (SAM)s alkanethiolates terminated diethylene glycol and carboxylic groups, poly(ethylene glycol) (PEG) grafted onto SAMs, zwitterionic polymer brushes poly(carboxybetaine methacrylate), poly(sulfobetaine...
Ultra-low-fouling poly[N-(2-hydroxypropyl) methacrylamide] (poly(HPMA)) brushes have been synthesized for the first time. Similar to so far only ultra-low-fouling surface, poly(carboxybetaine acrylamide), level of blood plasma fouling was below detection limit surface plasmon resonance (SPR, 0.03 ng·cm(-2)) despite being a hydrogen bond donor and displaying moderate wettability, thus challenging currently accepted views design antifouling properties. The properties were preserved even after...
Bioinspired poly(dopamine) (PDA) films are merged with antifouling poly(MeOEGMA) brushes utilizing a nitrile imine-mediated tetrazole-ene cycloaddition (NITEC)-based phototriggered surface encoding protocol. The were photopatterned on PDA surfaces, leading cells to form confluent layers in the non-irradiated sections, while no adhesion occurred resulting remarkably precise cell pattern. presented strategy paves way for design of tailor-made patterned interfaces. As service our authors and...
The resistance of poly(ethylene glycol) (PEG) against protein adsorption is crucial and has been widely utilized in various biomedical applications. In this work, the complete composition biofilms deposited on PEG-based surfaces from human blood plasma (BP) was identified for first time using nanoLC-MS/MS, a powerful tool analysis. mass BP number different proteins contained deposits individual decreased order self-assembling monolayers oligo(ethylene alkanethiolates (SAM) > end-grafted onto...
Abstract The fouling from four human body fluids – blood plasma, cerebrospinal fluid, urine and saliva animal foetal bovine calf sera, egg milk relevant to veterinary medicine, immunology, biology diagnostics is assessed on antifouling SAMs polymer brushes of oligo(ethylene glycol) methacrylate, 2‐hydroxyethyl carboxybetaine acrylamide N ‐(2‐hydroxypropyl)methacrylamide synthesized via ATRP. While important deposits the all biofluids are observed SAMs, a superior resistance achieved brushes....
In this study, we propose a substrate-independent biomimetic modification route for the creation of antifouling polymer brushes. This consists formation/deposition polydopamine anchor layer followed by well-controlled surface-initiated atom transfer radical polymerization brushes initiated 2-bromo-2-methylpropanoyl groups covalently attached to hydroxyl and amine present in layer. way, synthesized methoxy- hydroxy-capped oligoethylene glycol methacrylate, 2-hydroxyethyl methacrylate...
Functional cellulose substrates with tetrazole moieties are generated to serve as universal platforms for the spatio-temporal immobilization of synthetic ultra-low fouling polymer brushes and protein species via a nitrile imine-mediated tetrazole-ene cycloaddition (NITEC)-based protocol. Poly(carboxybetaine acrylamide) grafted from initiators photo-patterned by NITEC utilizing single electron transfer living radical polymerization. Streptavidin is photo-immobilized remarkable efficiency,...
The contact of blood with artificial materials generally leads to immediate protein adsorption (fouling), which mediates subsequent biological processes such as platelet adhesion and activation leading thrombosis. Recent progress in the preparation surfaces able prevent fouling offers a potential avenue mitigate this undesirable effect. In present contribution, we have prepared several types state-of-the-art antifouling polymer brushes on polycarbonate plastic substrate, investigated their...
A new route for coating various substrates with antifouling polymer layers was developed. It consisted in deposition of an amino-rich adhesion layer by means RF magnetron sputtering Nylon 6,6 followed the well-controlled, surface-initiated atom transfer radical polymerization brushes initiated bromoisobutyrate covalently attached to amino groups present layer. Polymer hydroxy- and methoxy-capped oligoethyleneglycol methacrylate carboxybetaine acrylamide were grafted from initiator a 15 nm...
The adhesion forces between a single bacterial cell and different polymer brushes were measured directly with an atomic force microscope correlated their resistance to fouling.
Novel antifouling highly wettable hydrogels with superior mechanical and self-healing properties are presented. Hydrogels were prepared by UV-initiated copolymerisation of non-fouling zwitterionic carboxybetaine methacrylamide (CBMAA-3) 2-hydroxyethyl methacrylate (HEMA) in the presence uniformly dispersed clay nanoparticles (Laponite XLG) water. The acted as physical cross-linkers resulting excellent resistance. effects composition such amount nanoclay HEMA/CBMAA-3 molar ratio on...
Implantable sensor devices require coatings that efficiently interface with the tissue environment to mediate biochemical analysis. In this regard, bioinspired polymer hydrogels offer an attractive and abundant source of coating materials. However, upon implantation these materials generally elicit inflammation foreign body reaction as a consequence protein fouling on their surface concomitant poor hemocompatibility. report we investigate strategy endow chitosan hydrogel antifouling...
Surface-initiated photoinduced SET-LRP of <italic>N</italic>-(2-hydroxypropyl)methacrylamide from an initiator-containing monolayer yields micropatterned brushes resistant to blood plasma fouling.
Abstract Nonthrombogenic modifications of membranes for extracorporeal membrane oxygenators (ECMOs) are key interest. The absence hemocompatibility these and the need anticoagulation patients result in severe potentially life‐threatening complications during ECMO treatment. To address lack membrane, surface developed, which act as barriers to protein adsorption on and, this way, prevent activation coagulation cascade. based nonionic zwitterionic polymer brushes grafted directly from...
Significance Glycan-lectin recognition is vital to embryogenesis, and progression in common diseases such as cancer osteoarthritis. However, the rules governing specificity for functional pairing postbinding events are not yet understood, even though human lectins have been thoroughly characterized structurally. Herein, we employ protein engineering produce new types of variants via redesign modular lectin architecture. Comparative assays with cells (for growth regulation)...
Significance The seminal fluid mosaic model of the cell membranes suggests a lipid bilayer sea, in which cholesterol, proteins, glycoconjugates, and other components are swimming. Complementing this view, microsegregated rafts predicts clusters that function as relay stations for intracellular signaling trafficking. However, elucidating arrangement glycoconjugates responsible communication recognition between cells, cells with proteins remains challenge. Herein, designed dendritic...
The principles for the selection of stereochemistry phospholipids biological membranes remain unclear and continue to be debated. Therefore, any new experiments on this topic may help progress in field. To address question, three libraries constitutional isomeric glycerol-amphiphilic Janus dendrimers (JDs) with nonsymmetric homochiral, racemic, symmetric achiral branching points were synthesized by an orthogonal-modular-convergent methodology. These JDs amplify self-assembly, therefore,...
Abstract Surface plasmon resonance (SPR) is a key technique in developing sensor platforms for clinical diagnostics, drug discovery, food quality, and environmental monitoring applications. While prism‐coupled (Kretschmann) SPR remains “gold‐standard” laboratory work‐flows due to easier fabrication, handling high through put, other configurations such as grating‐coupled (GC‐SPR) wave‐guide mode are yet fulfil their technology potential. This work evaluates the technical aspects influencing...
Five poly(betaine) brushes were prepared, and their resistance to blood plasma fouling was studied. Two carboxybetaines monomers copolymerized with 2-hydroxyethyl methacrylate (HEMA) prepare novel hydrogels. By increasing the content of zwitterionic comonomer, a 4-fold increase in water could be achieved while retaining mechanical properties close widely used poly(HEMA) All hydrogels showed an unprecedentedly low from plasma. Remarkably, by copolymerization 10 mol % carboxybetaine...
Cu(0) wire-catalyzed SET-LRP of N-(2-hydroxypropyl)methacrylamide (HPMA) initiated with methyl 2-chloropropionate (MCP) in H2O at 50 °C exhibited linear kinetics up to 90% conversion using 0.5 equivalents Me6-TREN respect initiator concentration.