A5056785882- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Toxin Mechanisms and Immunotoxins
- Enzyme-mediated dye degradation
- Enzyme Structure and Function
- Organometallic Complex Synthesis and Catalysis
- Crystallization and Solubility Studies
- Antibiotic Resistance in Bacteria
- X-ray Diffraction in Crystallography
- Synthetic Organic Chemistry Methods
- Metal complexes synthesis and properties
- Metal-Catalyzed Oxygenation Mechanisms
- Signaling Pathways in Disease
- Enzyme Production and Characterization
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolism and Applications
- Asymmetric Hydrogenation and Catalysis
- Protein Structure and Dynamics
- Chromium effects and bioremediation
- Vibrio bacteria research studies
- Pharmacological Effects of Natural Compounds
- Ferrocene Chemistry and Applications
- Escherichia coli research studies
- Biochemical and biochemical processes
- Pneumonia and Respiratory Infections
Centro de Investigaciones Biológicas Margarita Salas
2015-2024
Centro Nacional de Biotecnología
2024
Consejo Superior de Investigaciones Científicas
2008-2021
Complejo Hospitalario Universitario de Granada
2020
Universidad Complutense de Madrid
2016
Ludwig-Maximilians-Universität München
2015
University of Illinois Chicago
2011
Institute of Biostructure and Bioimaging
2011
Instituto de Investigación Biomédica de A Coruña
2010
Southern Methodist University
2010
The genome of Pleurotus ostreatus, an important edible mushroom and a model ligninolytic organism interest in lignocellulose biorefineries due to its ability delignify agricultural wastes, was sequenced with the purpose identifying characterizing enzymes responsible for lignin degradation.Heterologous expression class II peroxidase genes, followed by kinetic studies, enabled their functional classification. resulting inventory revealed absence peroxidases (LiPs) presence three versatile...
Acinetobacter baumannii is an extracellular opportunistic human pathogen that becoming increasingly problematic in hospitals. In the present study, we demonstrate A. Omp 33- to 36-kDa protein (Omp33-36) a porin acts as channel for passage of water. The found on cell surface and released along with other porins outer membrane vesicles (OMVs). immune connective tissue, this induced apoptosis by activation caspases modulation autophagy, consequent accumulation p62/SQSTM1 (sequestosome 1)...
Combating bacterial resistance to beta-lactams, the most widely used antibiotics, is an emergent and clinically important challenge. OXA-24 a class D beta-lactamase isolated from multiresistant epidemic clinical strain of Acinetobacter baumannii. We have investigated how specifically hydrolyzes last resort carbapenem antibiotic, we determined crystal structure at resolution 2.5 A. The shows that carbapenem's substrate specificity by hydrophobic barrier established through specific...
The wide utilization of biocides poses a concern on the impact these compounds natural bacterial populations. Furthermore, it has been demonstrated that can select, at least in laboratory experiments, antibiotic resistant bacteria. This situation raised concerns, not just scientists and clinicians, but also regulatory agencies, which are demanding studies may have development resistance consequently treatment infectious diseases human health. In present article, we explored possibility...
Dye-decolorizing peroxidase (DyP) of Auricularia auricula-judae has been expressed in Escherichia coli as a representative new DyP family, and subjected to mutagenic, spectroscopic, crystallographic computational studies. The crystal structure shows buried haem cofactor, surface tryptophan tyrosine residues potentially involved long-range electron transfer from bulky dyes. Simulations using PELE (Protein Energy Landscape Exploration) software provided several binding-energy optima for the...
Significance Glycan-lectin recognition is vital to embryogenesis, and progression in common diseases such as cancer osteoarthritis. However, the rules governing specificity for functional pairing postbinding events are not yet understood, even though human lectins have been thoroughly characterized structurally. Herein, we employ protein engineering produce new types of variants via redesign modular lectin architecture. Comparative assays with cells (for growth regulation)...
Lignin biodegradation, a key step in carbon recycling land ecosystems, is carried out by white-rot fungi through an H(2)O(2)-dependent process defined as enzymatic combustion. Pleurotus eryngii selective lignin-degrading fungus that produces H(2)O(2) during redox cycling of p-anisylic compounds involving the secreted flavoenzyme aryl-alcohol oxidase (AAO). Here, 2.4 A resolution X-ray crystal structure this oxidoreductase, which catalyzes dehydrogenation reactions on various primary...
ABSTRACT Control of membrane permeability is a key step in regulating the intracellular concentration antibiotics. Efflux pumps confer innate resistance to wide range toxic compounds such as antibiotics, dyes, detergents, and disinfectants members Enterobacteriaceae . The AcrAB-TolC efflux pump involved multidrug Enterobacter cloacae However, underlying mechanism that regulates system this microorganism remains unknown. In Escherichia coli , transcription acrAB upregulated under global...
Versatile peroxidase shares with manganese and lignin the ability to oxidize Mn2+ high redox potential aromatic compounds, respectively. Moreover, it is also able phenols (and low dyes) at two catalytic sites, as shown by biphasic kinetics. A efficiency site (with 2,6-dimethoxyphenol p-hydroquinone efficiencies of ∼70 ∼700 s−1 mm−1, respectively) was localized same exposed Trp-164 responsible for substrate oxidation (as activity loss in W164S variant). The second site, characterized (∼3 ∼50...
Class D β-lactamases represent a growing and diverse class of penicillin-inactivating enzymes that are usually resistant to commercial β-lactamase inhibitors. As many such found in multi-drug (MDR) Acinetobacter baumannii Pseudomonas aeruginosa, novel inhibitors urgently needed. Five unique 6-alkylidene-2′-substituted penicillanic acid sulfones (1−5) were synthesized tested against OXA-24, clinically important inactivates carbapenems is A. baumannii. Based upon the roles Tyr112 Met223 play...