A5073561477- Enzyme-mediated dye degradation
- Biochemical and biochemical processes
- Enzyme Catalysis and Immobilization
- Lignin and Wood Chemistry
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Cell death mechanisms and regulation
- Polyamine Metabolism and Applications
- Fungal Biology and Applications
- Amino Acid Enzymes and Metabolism
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Fermentation and Sensory Analysis
- Mycorrhizal Fungi and Plant Interactions
- Photosynthetic Processes and Mechanisms
- Microbial metabolism and enzyme function
- X-ray Diffraction in Crystallography
- Electrochemical sensors and biosensors
- Growth and nutrition in plants
- Crystallization and Solubility Studies
- Photoreceptor and optogenetics research
- Microbial Metabolism and Applications
- Drug-Induced Hepatotoxicity and Protection
- Pharmacogenetics and Drug Metabolism
- Enzyme Structure and Function
Universidad de Zaragoza
2015-2025
Universidade Federal do Paraná
2022
Pontifical Catholic University of Rio de Janeiro
2020
Centro Médico Sanitas Zaragoza
2017
Instituto de Biocomputación y Física de Sistemas Complejos
2012-2015
State University of Norte Fluminense
2011
Centro de Investigaciones Biológicas Margarita Salas
2005-2009
Consejo Superior de Investigaciones Científicas
2005-2009
Drexel University
2006-2009
Eucalyptus Research Center
2007
Wood is a major pool of organic carbon that highly resistant to decay, owing largely the presence lignin. The only organisms capable substantial lignin decay are white rot fungi in Agaricomycetes, which also contains non-lignin-degrading brown and ectomycorrhizal species. Comparative analyses 31 fungal genomes (12 generated for this study) suggest lignin-degrading peroxidases expanded lineage leading ancestor reconstructed as species, then contracted parallel lineages mycorrhizal Molecular...
Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and also largely responsible for the destructive decay wooden structures. Rapid depolymerization cellulose is a distinguishing feature brown-rot, but biochemical mechanisms underlying genetics poorly understood. Systematic examination P. genome, transcriptome, secretome revealed unique extracellular enzyme systems, including an unusual repertoire glycoside hydrolases. Genes encoding exocellobiohydrolases...
Efficient lignin depolymerization is unique to the wood decay basidiomycetes, collectively referred as white rot fungi. Phanerochaete chrysosporium simultaneously degrades and cellulose, whereas closely related species, Ceriporiopsis subvermispora, also depolymerizes but may do so with relatively little cellulose degradation. To investigate basis for selective ligninolysis, we conducted comparative genome analysis of C. subvermispora P. . Genes encoding manganese peroxidase numbered 13 five...
Oxidative conversion of 5‐hydroxymethylfurfural ( HMF ) is biotechnological interest for the production renewable (lignocellulose‐based) platform chemicals, such as 2,5‐furandicarboxylic acid FDCA ). To best our knowledge, ability fungal aryl‐alcohol oxidase AAO to oxidize reported here first time, resulting in almost complete into 2,5‐formylfurancarboxylic FFCA a few hours. The reaction starts with alcohol oxidation, yielding 2,5‐diformylfuran DFF ), which rapidly converted by carbonyl most...
Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable colonizing freshly exposed conifer sapwood despite its high content extractives, which retards establishment other fungal species. The mechanism(s) P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report annotated genome compare...
Abstract As actors of global carbon cycle, Agaricomycetes (Basidiomycota) have developed complex enzymatic machineries that allow them to decompose all plant polymers, including lignin. Among them, saprotrophic Agaricales are characterized by an unparalleled diversity habitats and lifestyles. Comparative analysis 52 genomes (14 sequenced de novo) reveals possess a large hydrolytic oxidative enzymes for lignocellulose decay. Based on the gene families with predicted highest evolutionary...
2,5-Furandicarboxylic acid is a renewable building block for the production of polyfurandicarboxylates, which are biodegradable polyesters expected to substitute their classical counterparts derived from fossil resources. It may be produced bio-based 5-hydroxymethylfurfural or 5-methoxymethylfurfural, both obtained by acidic dehydration biomass-derived fructose. 5-Methoxymethylfurfural, in presence methanol, generates less by-products and exhibits better storage stability than being,...
Lentinula is a broadly distributed group of fungi that contains the cultivated shiitake mushroom, L. edodes . We sequenced 24 genomes representing eight described species and several unnamed lineages from 15 countries on four continents. comprises major clades arose in Oligocene, three Americas one Asia–Australasia. To expand sampling mushrooms, we assembled 60 China were previously published as raw Illumina reads added them to our dataset. sensu lato (s. lat.) may warrant recognition...
Spectral and catalytic properties of the flavoenzyme AAO (aryl-alcohol oxidase) from Pleurotus eryngii were investigated using recombinant enzyme. Unlike most flavoprotein oxidases, does not thermodynamically stabilize a flavin semiquinone radical forms no sulphite adduct. catalyses oxidative dehydrogenation wide range unsaturated primary alcohols with hydrogen peroxide production. This differentiates enzyme VAO (vanillyl-alcohol oxidase), which is specific for phenolic compounds. Moreover,...
Lignin biodegradation, a key step in carbon recycling land ecosystems, is carried out by white-rot fungi through an H(2)O(2)-dependent process defined as enzymatic combustion. Pleurotus eryngii selective lignin-degrading fungus that produces H(2)O(2) during redox cycling of p-anisylic compounds involving the secreted flavoenzyme aryl-alcohol oxidase (AAO). Here, 2.4 A resolution X-ray crystal structure this oxidoreductase, which catalyzes dehydrogenation reactions on various primary...
Fungal AAO (aryl-alcohol oxidase) provides H2O2 for lignin biodegradation. is active on benzyl alcohols that are oxidized to aldehydes. However, during oxidation of some alcohols, forms more than a stoichiometric number molecules with respect the amount aldehyde detected due double reaction involves aryl-aldehyde oxidase activity. The latter was investigated using different benzylic aldehydes, whose acids demonstrated by GC-MS. steady- and presteady state kinetic constants, together...
AAO (aryl-alcohol oxidase) provides H₂O₂ in fungal degradation of lignin, a process high biotechnological interest. The crystal structure does not show open access to the active site, where different aromatic alcohols are oxidized. In present study we investigated substrate diffusion and oxidation compared with structurally related CHO (choline oxidase). Cavity finder ligand simulations indicate substrate-entrance channel, requiring side-chain displacements involving stacking interaction...
The crystal structure of aryl-alcohol oxidase (AAO), a flavoenzyme involved in lignin degradation, reveals two active-site histidines, whose role the enzyme half-reactions was investigated. redox state flavin during turnover variants obtained show stronger histidine involvement reductive than oxidative half-reaction. This confirmed by kcat/Km(Al) and reduction constants that are 2–3 orders magnitude decreased for His546 up to 5 His502 variants, while corresponding O2 only 1 order magnitude....
The genomes of three representative Polyporales (Bjerkandera adusta, Phlebia brevispora and a member the Ganoderma lucidum complex) recently were sequenced to expand our knowledge on diversity distribution genes involved in degradation plant polymers this Basidiomycota order, which includes most wood-rotting fungi. Oxidases, including members glucose-methanol-choline (GMC) oxidoreductase superfamily, play central role above degradative process because they generate extracellular H2O2 acting...
The apoptosis-inducing factor (AIF) is a mitochondrial-flavoprotein that, after cell death induction, distributed to the nucleus mediate chromatinolysis. In mitochondria, AIF present in monomer-dimer equilibrium that reduction by NADH gets displaced toward dimer. crystal structure of human (hAIF):NAD(H)-bound dimer revealed one FAD and, unexpectedly, two NAD(H) molecules per protomer. A 1:2 hAIF:NAD(H) binding stoichiometry was additionally confirmed solution using surface plasmon resonance....
ABSTRACT Auxiliary activities family 3 subfamily 2 (AA3_2) from the CAZy database comprises various functions related to ligninolytic enzymes, such as fungal aryl alcohol oxidases (AAO) and glucose oxidases, both of which are flavoenzymes. The recent study Pycnoporus cinnabarinus CIRM BRFM 137 genome combined with its secretome revealed that four AA3_2 enzymes secreted during biomass degradation. One these scf184803.g17, has recently been produced heterologously in Aspergillus niger . Based...
Abstract The Apoptosis-Inducing Factor (AIF) is a moonlighting flavoenzyme involved in the assembly of mitochondrial respiratory complexes healthy cells, but also able to trigger DNA cleavage and parthanatos. Upon apoptotic-stimuli, AIF redistributes from mitochondria nucleus, where upon association with other proteins such as endonuclease CypA histone H2AX, it proposed organize DNA–degradosome complex. In this work, we provide evidence for molecular complex well cooperative effects among...
Abstract Primary alcohol oxidation by aryl‐alcohol oxidase (AAO), a flavoenzyme providing H 2 O to ligninolytic peroxidases, is produced concerted proton and hydride transfers, as shown substrate solvent kinetic isotope effects (KIEs). Interestingly, when the reaction was investigated with synthesized ( R )‐ S )‐α‐deuterated p ‐methoxybenzyl alcohol, primary KIE (≈6) observed only for enantiomer, revealing that transfer highly stereoselective. Docking of at buried crystal active site,...
Aryl-alcohol oxidase (AAO) is an extracellular flavoprotein that supplies ligninolytic peroxidases with H2O2 during natural wood decay. With a broad substrate specificity and highly stereoselective reaction mechanism, AAO attractive candidate for studies into organic synthesis synthetic biology, yet the lack of suitable heterologous expression systems has precluded its engineering by directed evolution. In this study, native signal sequence from Pleurotus eryngii was replaced those mating...