A5031318965- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Mycobacterium research and diagnosis
- Mycorrhizal Fungi and Plant Interactions
- Plant-Microbe Interactions and Immunity
- Insect symbiosis and bacterial influences
- Legume Nitrogen Fixing Symbiosis
- Gut microbiota and health
- Plant Pathogens and Fungal Diseases
- Enzyme Structure and Function
- Plant Pathogenic Bacteria Studies
- Mycotoxins in Agriculture and Food
- Metalloenzymes and iron-sulfur proteins
- Chemical Synthesis and Analysis
- Genomics and Phylogenetic Studies
- Nematode management and characterization studies
- Biochemical and Molecular Research
- Fungal and yeast genetics research
- Ginseng Biological Effects and Applications
- Structural Health Monitoring Techniques
- Peptidase Inhibition and Analysis
- Plant biochemistry and biosynthesis
- Non-Destructive Testing Techniques
- Carbohydrate Chemistry and Synthesis
- Biochemical and Structural Characterization
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2016-2025
University of Bayreuth
2024-2025
Friedrich Schiller University Jena
2013-2025
Medizinische Hochschule Hannover
2024
German Center for Lung Research
2024
Helmholtz Centre for Infection Research
2024
TÜV (Austria)
2022-2024
ETH Zurich
2015-2019
Schiller (France)
2019
Board of the Swiss Federal Institutes of Technology
2017
Summary Ectomycorrhizal fungi are thought to have a key role in mobilizing organic nitrogen that is trapped soil matter ( SOM ). However, the extent which ectomycorrhizal decompose and mechanism by they do so remain unclear, considering lost many genes encoding lignocellulose‐degrading enzymes present their saprotrophic ancestors. Spectroscopic analyses transcriptome profiling were used examine mechanisms five species of fungi, representing at least four origins symbiosis, extracted from...
Clonostachys rosea is a mycoparasitic fungus that can control several important plant diseases. Here, we report on the genome sequencing of C. and comparative analysis, in order to resolve phylogenetic placement study evolution mycoparasitism as fungal lifestyle. The estimated 58.3 Mb, contains 14,268 predicted genes. A phylogenomic analysis shows clusters sister taxon pathogenic Fusarium species, with mycoparasitic/saprotrophic Trichoderma species an ancestral position. gene family reveals...
Significance The candidate genus “ Candidatus Entotheonella” belongs to a recently proposed bacterial phylum with largely unknown properties due the lack of cultivated members. Among few known biological is an association Ca . Entotheonella marine sponges and extraordinarily rich genomic potential for bioactive natural products unique structures unprecedented biosynthetic enzymology. Increasing evidence suggests that are widespread key producers sponge chemical richness comparable soil...
The rice seedling blight fungus Rhizopus microsporus and its endosymbiont Burkholderia rhizoxinica form an unusual, highly specific alliance to produce the potent antimitotic phytotoxin rhizoxin. Yet, it has remained a riddle how bacteria invade fungal cells. Genome mining for potential symbiosis factors functional analyses revealed that type 2 secretion system (T2SS) of bacterial is required formation endosymbiosis. Comparative proteome show T2SS releases chitinolytic enzymes (chitinase,...
Abstract Aspirochlorine ( 1 ) is an epidithiodiketopiperazine (ETP) toxin produced from koji mold (Aspergillus oryzae), which has been used in the oriental cuisine for over two millennia. Considering its potential risk food safety, we have elucidated molecular basis of aspirochlorine biosynthesis. By a combination genetic and chemical analyses found acl gene locus identified key role AclH as chlorinase. Stable isotope labeling, biotransformation, mutational experiments, analysis...
Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable colonizing freshly exposed conifer sapwood despite its high content extractives, which retards establishment other fungal species. The mechanism(s) P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report annotated genome compare...
Mycofactocin is a redox cofactor essential for the alcohol metabolism of mycobacteria. While biosynthesis mycofactocin well established, gene mftG , which encodes an oxidoreductase glucose-methanol-choline superfamily, remained functionally uncharacterized. Here, we show that MftG enzymes are almost exclusively found in genomes containing biosynthetic genes and present 75% organisms harboring these genes. Gene deletion experiments Mycolicibacterium smegmatis demonstrated growth defect ∆...
Burkholderia rhizoxinica is an intracellular symbiont of the phytopathogenic zygomycete Rhizopus microsporus, causative agent rice seedling blight. The endosymbiont produces antimitotic macrolide rhizoxin for its host. It vertically transmitted within vegetative spores and essential spore formation fungus. To shed light on evolution genetic potential this model organism, we analysed whole genome B. HKI 0454 - a type strain endofungal species.The consists structurally conserved chromosome two...
ABSTRACT Burkholderia rhizoxinica is an intracellular symbiont of the phytopathogenic fungus Rhizopus microsporus . The vertically transmitted endosymbiont not only delivers antimitotic macrolide rhizoxin to its host but also essential for vegetative spore formation fungus. To shed light on genetic equipment this model organism, we sequenced whole genome B. HKI 0454, thus providing first genomic insight into mutualist a fungal species. 3.75-Mb consists chromosome and two strain-specific...
Division of labor: A combination genetic, microbial, and chemical analyses solved the riddle dual epoxidation in biosynthesis rhizoxin, causative agent rice seedling blight. Bacterial endosymbionts Rhizopus microsporus mediate first by a dedicated cytochrome P450 monooxygenase. The second oxirane ring is introduced fungal host results substantially increased potency phytotoxin. Detailed facts importance to specialist readers are published as "Supporting Information". Such documents...
ABSTRACT Toxinogenic endobacteria were isolated from a collection of Rhizopus spp. representing highly diverse geographic origins and ecological niches. All endosymbionts belonged to the Burkholderia rhizoxinica complex according matrix-assisted laser desorption ionization-time flight biotyping multilocus sequence typing, suggesting common ancestor. Comparison host symbiont phylogenies provides insights into possible cospeciation horizontal-transmission events.
Caught in the act: Imaging mass spectrometry of a button mushroom (see picture) infected with soft rot pathogen Janthinobacterium agaricidamnosum conjunction genome mining revealed jagaricin as highly antifungal virulence factor that is not produced under standard cultivation conditions. The structure was rigorously elucidated by combination physicochemical analyses, chemical derivatization, and bioinformatics.
Upon injury, psychotropic psilocybin-producing mushrooms instantly develop an intense blue color, the chemical basis and mode of formation which has remained elusive. We report two enzymes from Psilocybe cubensis that carry out a two-step cascade to prepare psilocybin for oxidative oligomerization leads products. The phosphatase PsiP removes 4-O-phosphate group yield psilocin, while PsiL oxidizes its 4-hydroxy group. reaction was monitored by in situ 13 C NMR spectroscopy, indicated coupling...
The benastatins, pradimicins, fredericamycins, and members of the griseorhodin/rubromycin family represent a structurally functionally diverse group long-chain polyphenols from actinomycetes. Comparison their biosynthetic gene clusters (ben, prm, fdm, grh, rub) revealed that all loci harbor genes coding for similar, yet uncharacterized, type ketoreductases. In phylogenetic survey representative KRs involved in II PKS systems, we found it is generally possible to deduce KR regiospecificity...
Dress code for living in a fungus: Analysis of the carbohydrate coating toxin-producing endobacterium phytopathogenic fungus Rhizopus microsporus revealed an unprecedented lipopolysaccharide (LPS) structure, which is important infection and colonization fungal host. A mutant lacking unusual [→2)-β-D-galactofuranose-(1→]n O antigen (red schematic illustration) was incapable forming stable symbiosis with fungus. Detailed facts importance to specialist readers are published as "Supporting...
The filamentous mould Rhizopus microsporus is a member of the zygomycetes (lower fungi).While some strains serve as food fermenting fungi, others represent infamous plant pathogens and opportunistic human pathogens.Recently, it was shown that R. are associated with symbiotic bacteria.Here, we outline why these organisms important for health how they can be exploited drug development.Furthermore, illustrate what investigation bacterial-fungal symbiosis teach us about evolution pathogenicity...
Abstract The endosymbiosis between the pathogenic fungus Rhizopus microsporus and toxin-producing bacterium Mycetohabitans rhizoxinica represents a unique example of host control by an endosymbiont. Fungal sporulation strictly depends on presence endosymbionts as well bacterially produced secondary metabolites. However, influence primary metabolites remained unexplored. Recently, we discovered that M. produces FO 3PG-F420, derivative specialized redox cofactor F420. Whether FO/3PG-F420 plays...
Killing them softly. The molecular basis for the biosynthesis of tolaasin, Pseudomonas tolaasii-derived causative agent mushroom (Agaricus bisporus) soft rot, was revealed by genome mining and analysis NRPS genes. Production toxic lipopeptides during infection confirmed imaging mass spectrometry. As a service to our authors readers, this journal provides supporting information supplied authors. Such materials are peer reviewed may be re-organized online delivery, but not copy-edited or...
Abstract Ribosomally synthesized and posttranslationally modified peptide natural products (RiPPs) exhibit diverse structures bioactivities are classified into distinct biosynthetic families. A recently reported family is the proteusins, with prototype members polytheonamides being generated by almost 50 maturation steps, including introduction of d ‐residues at multiple positions an unusual radical SAM epimerase. region in protein‐like N‐terminal leader proteusin precursors identified that...
A key step in fungal L-lysine biosynthesis is catalyzed by adenylate-forming L-α-aminoadipic acid reductases, organized domains for adenylation, thiolation, and the reduction step. However, genomes of numerous ascomycetes basidiomycetes contain an unexpectedly large number additional genes encoding similar but functionally distinct enzymes. Here, we describe functional vitro characterization four reductases which were heterologously produced Escherichia coli. The Ceriporiopsis subvermispora...