A5014577624- Genetic diversity and population structure
- Animal Ecology and Behavior Studies
- Evolution and Paleontology Studies
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Bat Biology and Ecology Studies
- Genetic Mapping and Diversity in Plants and Animals
- Yersinia bacterium, plague, ectoparasites research
- Wildlife Ecology and Conservation
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- melanin and skin pigmentation
- Chromosomal and Genetic Variations
- RNA regulation and disease
- Biochemical Analysis and Sensing Techniques
- Identification and Quantification in Food
- Genetic and phenotypic traits in livestock
- Enzyme-mediated dye degradation
- Isotope Analysis in Ecology
- Wind Energy Research and Development
- Species Distribution and Climate Change
- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antimicrobial Resistance in Staphylococcus
Hokkaido University
2016-2025
Juntendo University
2024
Juntendo University Urayasu Hospital
2024
Satou Hospital
2009-2020
University of Toronto
2012-2019
Japan Advanced Institute of Science and Technology
2009-2016
Environmental Earth Sciences
1999-2016
Institute of Biological Problems of the North
2015
Russian Academy of Sciences
2015
Severtsov Institute of Ecology and Evolution
2015
Besides linear RNAs, pre-mRNA splicing generates three forms of RNAs: lariat introns, Y-structure introns from trans-splicing, and circular exons through exon skipping. To study the persistence excised in total cellular RNA, we used Escherichia coli 3' to 5' exoribonucleases. Ribonuclease R (RNase R) thoroughly degrades abundant RNAs while preserving loop portion a RNA. II II) polynucleotide phosphorylase (PNPase) also preserve loop, but are less efficient degrading RNAs. RNase digestion RNA...
The Black Rat (Rattus rattus) spread out of Asia to become one the world's worst agricultural and urban pests, a reservoir or vector numerous zoonotic diseases, including devastating plague. Despite global scale inestimable cost their impacts on both human livelihoods natural ecosystems, little is known genetic diversity Rats, timing directions historical dispersals, risks associated with contemporary movements. We surveyed mitochondrial DNA Rats collected across range as first step towards...
Abstract Background Softwood is the predominant form of land plant biomass in Northern hemisphere, and among most recalcitrant resources to bioprocess technologies. The white rot fungus, Phanerochaete carnosa , has been isolated almost exclusively from softwoods, while other known white-rot species, including chrysosporium were mainly hardwoods. Accordingly, it anticipated that P. encodes a distinct set enzymes proteins promote softwood decomposition. To elucidate genetic basis bioconversion...
Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable colonizing freshly exposed conifer sapwood despite its high content extractives, which retards establishment other fungal species. The mechanism(s) P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report annotated genome compare...
The liver enzymes, alcohol dehydrogenase (ADH) and aldehyde de‐hydrogenase (ALDH), which are responsible for the oxidative metabolism of ethanol, polymorphic in humans. Cytochrome P450IIE1 , an ethanol‐inducible isozyme microsomal P450 is also important ethanol metabolism. Genetic polymorphisms 5′‐flanking region human cytochrome gene have recently been reported. We hypothesized that ADH ALDH modify susceptibility to development alcoholism. determined genotypes ADH2 ALDH2 loci 96 Japanese...
Phagocytic cells can be primed for enhanced stimulated release of superoxide anion (0;) by exposure to a variety biologic agents, including y-interferon and lipopolysaccharide.We examined the role calcium ion in this priming, using ionophore ionomycin.Preincubation with ionomycin, 1 10 nM, human neutrophils up 7-fold more 0; during stimulation MM formyl-methionylleucyl-phenylalanine (f-Met-Leu-Phe).With 160 nM phorbol myristate acetate as stimulus, ionomycin caused doubling production mouse...
Abstract For more than 100 years, house mice (Mus musculus) have been used as a key animal model in biomedical research. House are genetically diverse, yet their genetic background at the global level has not fully understood. Previous studies suggested that they originated South Asia and diverged into three major subspecies, almost simultaneously, approximately 110,000–500,000 years ago; however, spread across world with migration of modern humans prehistoric historic times (∼10,000 ago to...
Phylogenetic relationships among 20 species-group taxa of Mustelidae, representing Mustelinae (Mustela, Martes, Gulo), Lutrinae (Enhydra), and Melinae (Meles), were examined using nucleotide sequences the nuclear interphotoreceptor retinoid binding protein (IRBP) mitochondrial cytochrome b genes. Neighbor-joining maximum-parsimony phylogenetic analyses on these genes separately combined conducted. While IRBP performed better than in recovering more-inclusive clades, demonstrated more...
Considerable long-standing controversy and confusion surround the phylogenetic affinities of pinnipeds, largely marine group "fin-footed" members placental mammalian order Carnivora. Until most recently, two major competing hypotheses were that pinnipeds have a single (monophyletic) origin from bear-like ancestor, or they dual (diphyletic) origin, with sea lions (Otariidae) derived seals (Phocidae) an otter-, mustelid-, musteloid-like ancestor. We examined relationships among 29 species...
Genetic variability of the nontranscribed spacer (NTS) region within ribosomal DNA repeating units in various subspecies Mus musculus was determined. Mice belonging to several laboratory mouse strains were examined by means Southern blot hybridization with a probe. This probe encompasses 3' end 28S RNA (rRNA) gene and following spacer. Restriction enzyme digestions liver DNAs from wild mice revealed that each has unique pattern encompassing distance approximately 10 kb downstream gene. These...
PR/SET domain containing 9 (Prdm9) mediates histone modifications such as H3K4me3 and marks hotspots of meiotic recombination. In many mammalian species, the Prdm9 gene is highly polymorphic. polymorphism assumed to play two critical roles in evolution: diversify spectrum recombination cause male hybrid sterility, leading reproductive isolation speciation. Nevertheless, information about sequences natural populations very limited. this study, we conducted a comprehensive population survey on...
Determining reliable evolutionary rates of molecular markers is essential in illustrating historical episodes with phylogenetic inferences. Although emerging evidence has suggested a high rate for intraspecific genetic variation, it unclear how long such persist because recent calibration point rarely available. Other than using fossil evidence, possible to estimate by relying on the well-established temporal framework Quaternary glacial cycles that would likely have promoted both rapid...
Sequences of the mitochondrial cytochrome b (1140 bp) and nuclear IRBP (1152 genes were used to assess evolutionary history Apodemus, using complete set Asian species. Our results indicate that speciation in Asia involved three radiations, which supports an earlier study. The initial radiation yielded A. argenteus (Japanese endemic), gurkha (Nepalese ancestral lineage remaining This subsequently diverged into four groups: agrarius-chevrieri (agrarius group), draco-latronum-semotus (draco...
Phylogenetic relationships among the ferret-badger Melogale moschata, skunk Mephitis mephitis, and 21 other arctoid carnivorans, representing Mustelidae (Mustelinae: Mustela, Martes, Gulo; Lutrinae: Enhydra; Melinae: Meles), Procyonidae (Procyon), Ursidae (Ursus, Melursus), were evaluated through maximum-parsimony phylogenetic analysis of concatenated partial nucleotide sequences nuclear recombination-activating gene 1 (RAG1) encoding interphotoreceptor retinoid-binding protein (IRBP). The...
Fragments of the spacer region genes for rat 5S ribosomal RNA (rDNA), which are tandemly repeated, were amplified by PCR with primers specific to two ends coding rRNA. Two fragments ∼1.6 kb subcloned and sequenced. The sequences showed a high degree sequence identity each other (99.2%) but substantial divergence from those analogous mouse clones. homologous regions in clones interrupted duplication or deletion small segments DNA. A 12-mer, 5-GGCTCTTGGGGC-3’, thought be responsible efficient...