A5049548651- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Chromosomal and Genetic Variations
- Transgenic Plants and Applications
- RNA and protein synthesis mechanisms
- Plant and Fungal Interactions Research
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Viral Infectious Diseases and Gene Expression in Insects
- Photosynthetic Processes and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Pancreatic function and diabetes
- Protist diversity and phylogeny
- Bacterial Genetics and Biotechnology
- Diabetes Treatment and Management
- Neuropeptides and Animal Physiology
Tokyo University of the Arts
2008-2025
The University of Tokyo
2015-2025
Systems Biology Institute
2017-2022
Tokyo University of Science
1988-2020
Universal University
2017
Chugai Pharma (United States)
2009-2015
Rail Delivery Group
2014
RIKEN
2001-2010
Kyoto University
2009
RIKEN Advanced Science Institute
2000-2009
Meiosis ensures genetic diversification of gametes and sexual reproduction. For successful meiosis, multiple events such as DNA replication, recombination, chromosome segregation must occur coordinately in a strict regulated order. We investigated the meiotic roles Cdc7 kinase initiation namely, double-strand breaks (DSBs) mediated by Spo11 other coactivating proteins. Genetic analysis using bob1-1 cdc7Delta reveals that is essential for DSBs meiosis I progression. also demonstrate...
Glycerophospholipids, the structural components of cell membranes, have not been considered to be spatial cues for intercellular signaling because their ubiquitous distribution. We identified lyso-phosphatidyl-β-D-glucoside (LysoPtdGlc), a hydrophilic glycerophospholipid, and demonstrated its role in modality-specific repulsive guidance spinal cord sensory axons. LysoPtdGlc is locally synthesized released by radial glia patterned distribution regulate targeting nociceptive but proprioceptive...
The Mre11-Rad50-Nbs1(Xrs2) complex and the Ku70-Ku80 heterodimer are thought to compete with each other for binding DNA ends. To investigate mechanism underlying this competition, we analyzed both damage sensitivity telomere overhangs in Schizosaccharomyces pombe rad50-d, rad50-d pku70-d, exo1-d, pku70-d exo1-d cells. We found that rad50 exo1 double mutants more methyl methanesulfonate (MMS) sensitive than respective single mutants. MMS of cells was suppressed by concomitant deletion pku70+...
We investigated the biochemical characteristics of 51-kDa protein that is a major mitotic apparatus-associated basic sea urchin eggs (Toriyama, M., Ohta, K., Endo, S., and Sakai, H. (1988) Cell Motil. Cytoskeleton 9, 117-128). The amino acid composition was apparently different from those tubulin, actin, histones, myelin protein; yet it similar to polypeptide elongation factors 1 alpha (EF-1 alpha). In addition, antibody EF-1 yeast cross-reacted with protein. [3H] GTP binding activity...
Mitogen‐activated protein kinase (MAPK), a serine/threonine‐specific which is generally activated by stimulation with various growth factors and phorbol esters, utilizes microtubule‐associated (MAP) 2 as good substrate in vitro. We have found that MAPK‐catalyzed phosphorylation of MAP2 resulted significant loss its ability to induce tubulin polymerization. The chymotryptic fragments, containing microtubule‐binding domain MAP2, were phosphorylated MAPK the fragments polymerization was also...
Spo11-mediated DNA double-strand breaks (DSBs) that initiate meiotic recombination are temporally and spatially controlled. The cohesin Rec8 has been implicated in regulating DSB formation, but little is known about the features of their interplay. To elucidate this point, we investigated genome-wide localization Spo11 budding yeast during early meiosis by chromatin immunoprecipitation using high-density tiling arrays. We found dynamically localized to chromosomes. initially accumulated...
The evolutionarily conserved Mre11/Rad50/Nbs1 (MRN) complex is involved in various aspects of meiosis. Whereas available evidence suggests that the Mre11 nuclease activity might be responsible for Spo11 removal Saccharomyces cerevisiae, this has not been confirmed experimentally. This study demonstrates first time (Schizosaccharomyces pombe Rad32Mre11) required Rec12Spo11. Furthermore, we show CtIP homologue Ctp1 Rec12Spo11 removal, confirming functional conservation between Ctp1CtIP and...
During mild replication stress provoked by low dose aphidicolin (APH) treatment, the key Fanconi anemia protein FANCD2 accumulates on common fragile sites, observed as sister foci, and protects genome stability. To gain further insights into function its regulatory mechanisms, we examined genome-wide chromatin localization of in this setting ChIP-seq analysis. We found that mostly central regions a set large transcribed genes were extensively overlapped with known CFS. Consistent previous...
Histone modifications are associated with meiotic recombination hotspots, discrete sites augmented frequency. For example, trimethylation of histone H3 lysine4 (H3K4me3) marks most hotspots in budding yeast and mouse. Modified histones known to regulate partly by promoting DNA double-strand break (DSB) formation at but the role precise landscape involved remain unclear. Here, we studied hotspot-associated fission found general features: acetylation lysine9 (H3K9ac) is elevated, H3K4me3 not...
In the yeast Saccharomyces cerevisiae , meiotic recombination is initiated by DNA double-stranded breaks (DSBs) occurring in micrococcal nuclease (MNase)-hypersensitive regions of chromatin. MNase-sensitive sites also undergo meiosis-specific alterations chromatin structure prior to appearance DSBs. DSB formation requires products numerous genes. Herein we have examined effects mutations four such genes, MRE11 RAD50 XRS2 and MRE2 on MNase sensitivity at premeiotic cells. Disruption each...
PR/SET domain containing 9 (Prdm9) mediates histone modifications such as H3K4me3 and marks hotspots of meiotic recombination. In many mammalian species, the Prdm9 gene is highly polymorphic. polymorphism assumed to play two critical roles in evolution: diversify spectrum recombination cause male hybrid sterility, leading reproductive isolation speciation. Nevertheless, information about sequences natural populations very limited. this study, we conducted a comprehensive population survey on...
The subtelomere, a telomere-adjacent chromosomal domain, contains species-specific homologous DNA sequences, in addition to various genes. However, the functions of subtelomeres, particularly subtelomeric (SH) remain elusive. Here, we report first comprehensive analyses cellular SH sequences fission yeast, Schizosaccharomyces pombe. Complete removal from genome revealed that they are dispensable for mitosis, meiosis and telomere length control. when telomeres lost, prevent deleterious...