A5063889679- Analytical Chemistry and Chromatography
- Spectroscopy and Chemometric Analyses
- Advanced Chemical Sensor Technologies
- Acute Myeloid Leukemia Research
- Blood properties and coagulation
- Fermentation and Sensory Analysis
- Advanced Proteomics Techniques and Applications
- Phytochemicals and Antioxidant Activities
- Microfluidic and Capillary Electrophoresis Applications
- Erythrocyte Function and Pathophysiology
- Analytical chemistry methods development
- Platelet Disorders and Treatments
- Polymer Surface Interaction Studies
- Metabolomics and Mass Spectrometry Studies
- Meat and Animal Product Quality
- Mass Spectrometry Techniques and Applications
- Clusterin in disease pathology
- Analytical Methods in Pharmaceuticals
- Chromatography in Natural Products
- Advanced biosensing and bioanalysis techniques
- Hemoglobinopathies and Related Disorders
- Crystallization and Solubility Studies
- Horticultural and Viticultural Research
- Metal complexes synthesis and properties
- Adsorption, diffusion, and thermodynamic properties of materials
Slovak University of Technology in Bratislava
2010-2024
Czech Academy of Sciences, Institute of Analytical Chemistry
2011-2024
Institute of Haematology and Blood Transfusion
2013-2024
Research Institute for Chromatography
2008
Stellenbosch University
2008
The resistance of poly(ethylene glycol) (PEG) against protein adsorption is crucial and has been widely utilized in various biomedical applications. In this work, the complete composition biofilms deposited on PEG-based surfaces from human blood plasma (BP) was identified for first time using nanoLC-MS/MS, a powerful tool analysis. mass BP number different proteins contained deposits individual decreased order self-assembling monolayers oligo(ethylene alkanethiolates (SAM) > end-grafted onto...
A simple method for the analysis of major wine volatiles and semivolatiles by stir bar sorptive extraction in combination with thermal desorption gas chromatography−mass spectrometry (SBSE-TD-GC-MS) was developed. Significant experimental parameters such as time, temperature, salt addition, pH, were optimized to provide a sensitive robust analytical method. The provided good repeatability (%RSD < 10%) 38 volatile compounds, including alcohols, acids, esters, phenols, aldehydes, ketones,...
Platelets are small anucleated blood particles that play a key role in the control of bleeding. need to be activated perform their functions and participate hemostasis. The process activation is accompanied by vast protein reorganization posttranslational modifications. goal this study was identify changes proteins platelets different agonists. were three agonists - arachidonic acid, collagen, thrombin. 2D SDS-PAGE (pI 4-7) used separate platelet proteins. Proteomes resting compared with...
The aim of this proteomic study was to look for changes taking place in plasma proteomes patients with acute myocardial infarction (AMI), unstable angina pectoris (UAP), and stable (SAP). Depleted proteins were separated by 2D SDS-PAGE (pI 4-7), compared using Progenesis SameSpots statistical software. Proteins identified nanoLC-MS/MS. quantified commercial kits. Apolipoprotein A1 studied 1D SDS-PAGE, together western blotting. Reciprocal comparison revealed 46 unique, significantly...
A fibrin/heparin coating enriched with bound growth factors was developed and applied on an ePTFE vessel prosthesis. The exhibited excellent antithrombogenic properties supported endothelialization of the vessel.
Abnormal coagulation properties indicative of a dysfibrinogen were found in the plasma 72-year-old male with multiple myeloma (IgGĸ, stage IIIA). The patient had high paraprotein concentration (85.75 g/l) and prolonged thrombin time (76.8 s), activated partial thromboplastin (39.5 prothrombin (23.5 s) reptilase (72.0 s). fibrinogen level was increased. fibrin polymerization induced by both impaired. Scanning electron microscopy revealed abnormal clot morphology. After six months treatment,...
Oxidative stress in humans is related to various pathophysiological processes, which can manifest numerous diseases including cancer, cardiovascular diseases, and Alzheimer's disease. On the atomistic level, oxidative causes posttranslational modifications, thus inducing structural functional changes into proteins structure. This study focuses on fibrinogen, a blood plasma protein that frequently targeted by reagents causing modifications proteins. Fibrinogen was vitro modified three...
Whenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation complement or the coagulation cascade and adhesion leukocytes platelets leading to thrombus formation. Thus, considerable efforts directed towards preparation fouling-resistant surfaces best possible hemocompatibility. Herein, comprehensive hemocompatibility study after...