A5002324579- Vector-borne infectious diseases
- Mass Spectrometry Techniques and Applications
- Electrochemical Analysis and Applications
- Plant Molecular Biology Research
- Electrochemical sensors and biosensors
- Insect and Pesticide Research
- Photosynthetic Processes and Mechanisms
- Phytase and its Applications
- Proteins in Food Systems
- Analytical chemistry methods development
- Analytical Chemistry and Sensors
- Identification and Quantification in Food
- Protein Hydrolysis and Bioactive Peptides
- Viral Infections and Vectors
- Herpesvirus Infections and Treatments
- Microbial Metabolites in Food Biotechnology
- Mosquito-borne diseases and control
- Light effects on plants
- Biosensors and Analytical Detection
- Insect symbiosis and bacterial influences
- Vector-Borne Animal Diseases
- Microbial infections and disease research
- Biochemical and Molecular Research
- Toxin Mechanisms and Immunotoxins
- Gold and Silver Nanoparticles Synthesis and Applications
University of South Bohemia in České Budějovice
2019-2024
Institute of Parasitology
2023
Czech Academy of Sciences, Biology Centre
2023
Palacký University Olomouc
2017-2021
Czech Academy of Sciences, Institute of Plasma Physics
2019
Czech Academy of Sciences, Institute of Physics
2019
Czech Academy of Sciences, Institute of Analytical Chemistry
2010-2011
Masaryk University
2008
Cytokinins are plant hormones involved in regulation of diverse developmental and physiological processes plants whose molecular mechanisms action being intensely researched. However, most rapid responses to cytokinin signals at the proteomic phosphoproteomic levels unknown. Early were investigated through proteome-wide expression profiling based on image mass spectrometric analysis two-dimensionally separated proteins phosphoproteins. The effects 15 min treatments 7-day-old Arabidopsis...
New analytical techniques that overcome major drawbacks of current routinely used viral infection diagnosis methods, i.e., the long analysis time and laboriousness real-time reverse-transcription polymerase chain reaction (qRT-PCR) insufficient sensitivity "antigen tests", are urgently needed in context SARS-CoV-2 other highly contagious viruses. Here, we report on an antifouling terpolymer-brush biointerface enables rapid sensitive detection untreated clinical samples. The developed carries...
In the body of multicellular organisms, macrophages play an indispensable role in maintaining tissue homeostasis by removing old, apoptotic and damaged cells. addition, allow significant remodeling plans during embryonic morphogenesis, regeneration metamorphosis. Although huge amount organic matter that must be removed these processes represents a potential source nutrients, their further use organism has not yet been addressed. Here, we document that, metamorphosis, Drosophila larval...
Dormant cells of Mycobacterium tuberculosis , in addition to low metabolic activity and a high level drug resistance, are characterized by ‘non-culturability’–a specific reversible state the inability grow on solid media. The biochemical characterization this physiological pathogen is only superficial, pending clarification processes that may exist such cells. In study, applying LC-MS proteomic profiling, we report analysis proteins accumulated dormant, ‘non-culturable’ M . an vitro model...
Whenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation complement or the coagulation cascade and adhesion leukocytes platelets leading to thrombus formation. Thus, considerable efforts directed towards preparation fouling-resistant surfaces best possible hemocompatibility. Herein, comprehensive hemocompatibility study after...
Ticks are obligate hematophagous arthropods that transmit a wide range of pathogens to humans as well wild and domestic animals. They also harbor non-pathogenic microbiota, although our previous study has shown the diverse bacterial microbiome in midgut Ixodes ricinus is quantitatively poor lacks core. In artificial infections by capillary feeding ticks with two model bacteria (Gram-positive Micrococcus luteus Gram-negative Pantoea sp.), rapid clearance these microbes from was observed,...
Using a paraffin impregnated graphite electrode (PIGE) and mercury-modifiedpyrolytic with basal orientation (Hg-PGEb) copper(II) Cu(II)-DNApurine base solutions have been studied by cyclic (CV) linear sweep voltammetry(LSV) in connection elimination voltammetry scan (EVLS). In chlorideand bromide (pH 6), the redox process of Cu(II) proceeded on PIGE twocathodic two anodic potentially separated signals. According to eliminationfunction E4, first cathodic peak corresponds reduction e- → Cu(I)...
Abstract The major objective of proteomics is to identify and examine the large numbers proteins extracted from complex biological systems. This generally achieved by combining various techniques protein separation with a mass spectrometric analysis that are digested enzymatically. Recently, several alternatives this standard protocol have been developed for efficient fast digestion. One option use modified trypsin instead native in‐gel digestion proteins. Microwave, ultrasonic‐assisted...
Ticks are ectoparasites that feed on blood and have an impressive ability to consume process enormous amounts of host blood, allowing extremely long periods starvation between meals. The central role in the parasitic lifestyle ticks is played by midgut. This organ efficiently stores digests ingested serves as primary interface for transmission tick-borne pathogens. In this study, we used a label-free quantitative approach perform novel dynamic proteomic analysis midgut Ixodesricinus nymphs,...
Iripin-5 is the main Ixodes ricinus salivary serpin, which acts as a modulator of host defence mechanisms by impairing neutrophil migration, suppressing nitric oxide production macrophages and altering complement functions. influences immunity shows high expression in glands. Here, crystal structure most thermodynamically stable state serpins described. In reactive-centre loop, substrate-recognition site likely to be represented Arg342, implies targeting trypsin-like proteases. Furthermore,...
Abstract Prevention of fouling from proteins in blood plasma attracts significant efforts, and great progress is made identifying surface coatings that display antifouling properties. In particular, poly(ethylene glycol) (PEG) widely used dense PEG‐like cylindrical brushes poly[oligo(ethylene methacrylate] (poly(OEGMA)) can drastically reduce fouling. Herein, a comprehensive study the variation on this surface, including analysis composition protein deposits poly(OEGMA) after contact with...
This work aims at the utilization of nanostructured surfaces for advanced mass spectrometry [laser desorption/ionization (LDI MS)]. The authors demonstrate that prepared nanostructures enable independent mass-to-charge calibration and also effectively substitute protonation agent low-mass molecules instead conventionally used matrices. Silver were formed as homogeneous thin film, isolated nanoislands, spherical nanoparticles. Besides surface characterization, paper focuses on impact LDI MS...
Metal nanoparticles (NPs) were reported as an efficient matrix for detection of small molecules using laser desorption/ionization mass spectrometry. Their pronounced efficiency is mostly in desorption enhancement, while, some cases, NPs can facilitate charge transfer to a molecule, which has been alkali metals and silver. In this work, we present the study influence Ag NP size on spectra model analyte, molecule riboflavin. The produced by magnetron sputtering-based gas aggregation vacuum...
The availability of tick in vitro cell culture systems has facilitated many aspects research, including proteomics. However, certain lines have shown a tissue-specific response to infection. Thus, more thorough characterization is necessary. Proteomic comparative studies various will contribute efficient application as model for investigation host-vector-pathogen interactions. Three obtained from hard tick, Ixodes ricinus, and two I. scapularis were investigated. A mass spectrometry approach...
Trypsin is a protease, which commonly used for the digestion of protein samples in proteomic experiments. The process trypsin autolysis known to produce autolytic peptides as well active enzyme forms with one or more intra-chain splits. In consequence, their variable presence can influence substrate reaction mixture. Besides two major and well-studied named β-trypsin α-trypsin, there are also other such γ-trypsin pseudotrypsin (ψ-trypsin). this work, cleavage specificity ψ-trypsin was...
Abstract BACKGROUND: In barley, starch occurs in the form of granules with bimodal size distribution. Enzymatic hydrolysis granule is one most important reactions occurring during malting and mashing. Previous studies revealed discrepancies assumption that barley varieties better qualities should have a higher A/B (large/small granules) ratio. This led us to focus our attention on detailed analysis two varieties, Jersey Tolar, both high quality but significantly differing (1.28 0.66,...
This study demonstrates the use of nanoparticles prepared by a gas aggregation source for fabricating structures combining laser sintering and ablation. At first, morphology optical properties nanoparticle coatings were characterized. Then, response to irradiation at different powers or exposure times was studied in situ time-of-flight mass spectrometry, followed scanning electron microscopy measurements resulting structures. By comparing numbers detected Ag ions, that ablated desorbed, with...
Introduction: We developed a new simple method to assess the composition of proteinaceous components in saliva Ornithodoros moubata , main vehicle for pathogen transmission and likely source bioactive molecules acting at tick-vertebrate host interface. To collect naturally expectorated from ticks we employed an artificial membrane feeding technique using simple, chemically defined diet containing phagostimulants submitted native samples collected this way liquid chromatography-mass...