A5067990890- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
- Insect and Pesticide Research
- Toxin Mechanisms and Immunotoxins
- Insect Resistance and Genetics
- Trypanosoma species research and implications
- Neurobiology and Insect Physiology Research
- Mosquito-borne diseases and control
- Iron Metabolism and Disorders
- Insect Pest Control Strategies
- Study of Mite Species
- Entomopathogenic Microorganisms in Pest Control
- Plant Virus Research Studies
- Parasites and Host Interactions
- Antimicrobial Peptides and Activities
- Insect-Plant Interactions and Control
- Bird parasitology and diseases
- Parasite Biology and Host Interactions
- Plant Parasitism and Resistance
- Plant pathogens and resistance mechanisms
- Peptidase Inhibition and Analysis
- Helminth infection and control
Czech Academy of Sciences, Biology Centre
2015-2024
Czech Academy of Sciences
1999-2024
Institute of Parasitology
2015-2024
University of South Bohemia in České Budějovice
2000-2019
Institute of Parasitology of the Slovak Academy of Sciences
2018
Whitman College
2008
Georgia Institute of Technology
2008
University of California, San Francisco
2008
Institute of Entomology
1997
Uppsala University
1993
Ticks are among the most important vectors of a wide range human and animal diseases. During blood feeding, ticks exposed to an enormous amount free iron that must be appropriately used detoxified. However, mechanism metabolism in is poorly understood. Here, we show possess complex system efficiently utilizes, stores transports non-heme within tick body. We have characterized new secreted ferritin (FER2) regulatory protein (IRP1) from sheep tick, Ixodes ricinus , demonstrated their...
Haem and iron homeostasis in most eukaryotic cells is based on a balanced flux between haem biosynthesis oxygenase-mediated degradation. Unlike eukaryotes, ticks possess an incomplete biosynthetic pathway and, together with other (non-haematophagous) mites, lack gene encoding oxygenase. We demonstrated, by membrane feeding, that do not acquire bioavailable from haemoglobin-derived haem. However, require dietary haemoglobin as exogenous source of since, feeding haemoglobin-depleted serum led...
Abstract Adult females of the genus Ixodes imbibe blood meals exceeding about 100 times their own weight within 7‒9 days. During this period, ticks internalise components host by endocytic digest cells that line tick midgut epithelium. Using RNA-seq, we aimed to characterise transcriptome composition in adult ricinus during early and late phase engorgement. To address specific adaptations haemoglobin-rich diet, compared transcriptomes genetically homogenous female siblings fed either bovine...
Abstract Background Ticks are vectors of a wide variety pathogens causing severe diseases in humans and domestic animals. Intestinal digestion the host blood is an essential process tick physiology also limiting factor for pathogen transmission since gut represents primary site infection proliferation. Using model Ixodes ricinus , European Lyme disease vector, we have previously demonstrated by genetic biochemical analyses that degraded network acidic peptidases aspartic cysteine classes....
Ticks salivate while feeding on their hosts. Saliva helps blood through host anti-hemostatic and immunomodulatory components. Previous transcriptomic proteomic studies revealed the complexity of tick saliva, comprising hundreds polypeptides grouped in several multi-genic families such as lipocalins, Kunitz-domain containing peptides, metalloproteases, basic tail secreted proteins, other uniquely found ticks. These also that composition saliva changes with time; expression transcripts from...
A meeting sponsored by the Bill & Melinda Gates Foundation was held at Avanti Hotel, Mohammedia, Morocco, July 14-15, 2015. The resulted in formation of Cattle Tick Vaccine Consortium (CATVAC).
Culture-independent metagenomic methodologies have enabled detection and identification of microorganisms in various biological systems often revealed complex unknown microbiomes. In many organisms, the microbiome outnumbers host cells greatly affects biology fitness. Ticks are hematophagous ectoparasites with a wide range. They vector number human animal pathogens also directly cause major economic losses livestock. Although several reports on tick midgut microbiota show diverse bacterial...
A protein responsible for clot formation was isolated from plasma of the crayfish Pacifastacus leniusculus , by repeated percipitation at low ionic strength, pH 6.0. The protein, here named clotting (CP), is a lipoglycoprotein, which consists two 210‐kDa subunits, covalently associated disulfide bonds. Preparations CP can form stable clots in presence haemocyte lysate supernatant, contains endogenous, Ca 2+ ‐dependent transglutaminase (TGase) activity. covalent, TGase‐mediated polymerization...
Ticks are vectors for a variety of viral, bacterial and parasitic diseases in human domestic animals. To survive reproduce ticks feed on host blood, yet our understanding the intestinal proteolytic machinery used to derive absorbable nutrients from blood meal is poor. Intestinal digestive processes limiting factors pathogen transmission since tick gut presents primary site infection. Moreover, enzymes may find practical application as anti-tick vaccine targets.Using hard tick, Ixodes...
The saliva of blood-feeding parasites is a rich source peptidase inhibitors that help to overcome the host's defence during host–parasite interactions. Using proteomic analysis, cystatin OmC2 was demonstrated in soft tick Ornithodoros moubata, an important disease vector transmitting African swine fever virus and spirochaete Borrelia duttoni. A structural, biochemical biological characterization this inhibitor undertaken present study. Recombinant screened against panel physiologically...
In addition to being vectors of pathogenic bacteria, ticks also harbor intracellular bacteria that associate with over generations, aka symbionts. The biological significance such bacterial symbiosis has been described in several tick species but its function Ixodes ricinus is not understood. We have previously shown I. are primarily inhabited by a single symbiont, Midichloria mitochondrii, an bacterium resides and reproduces mainly the mitochondria ovaries fully engorged females. To study...
In this paper, we report the detection, purification and characterization of first metalloprotease inhibitor (IMPI) from invertebrates. IMPI was purified hemolymph last‐instar larvae Galleria mellonella by precipitation with trichloroacetic acid heat followed affinity chromatography on a thermolysin‐Sepharose column gel filtration or reverse‐phase high‐performance liquid chromatography. For detection activity, new azocoll assay established. only detectable in that had been injected bacterial...