A5059973854- Vector-borne infectious diseases
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- Insect and Pesticide Research
- Food Allergy and Anaphylaxis Research
- Tuberculosis Research and Epidemiology
- Toxin Mechanisms and Immunotoxins
- vaccines and immunoinformatics approaches
- Microbial infections and disease research
- Mycobacterium research and diagnosis
- Xenotransplantation and immune response
- SARS-CoV-2 and COVID-19 Research
- Bird parasitology and diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced Proteomics Techniques and Applications
- Adipose Tissue and Metabolism
- Peripheral Neuropathies and Disorders
- Study of Mite Species
- Hereditary Neurological Disorders
- Invertebrate Immune Response Mechanisms
- Cancer Mechanisms and Therapy
- interferon and immune responses
- Aquaculture disease management and microbiota
Instituto de Investigación en Recursos Cinegéticos
2016-2025
University of Castilla-La Mancha
2012-2025
Hospital General Universitario de Ciudad Real
2020
Consejo Superior de Investigaciones Científicas
2009-2019
Regional Government of Castile-La Mancha
2016-2019
AgroBio
2019
Unidades Centrales Científico-Técnicas
2017
Centro de Biología Molecular Severo Ochoa
2004-2008
Universidad Autónoma de Madrid
2005-2006
Molina Center for Energy and the Environment
2006
Abstract Ticks transmit more pathogens to humans and animals than any other arthropod. We describe the 2.1 Gbp nuclear genome of tick, Ixodes scapularis (Say), which vectors that cause Lyme disease, human granulocytic anaplasmosis, babesiosis diseases. The large reflects accumulation repetitive DNA, new lineages retro-transposons, gene architecture patterns resembling ancient metazoans rather pancrustaceans. Annotation scaffolds representing ∼57% genome, reveals 20,486 protein-coding genes...
Anaplasma phagocytophilum is an emerging zoonotic pathogen that causes human granulocytic anaplasmosis. These intracellular bacteria establish infection by affecting cell function in both the vertebrate host and tick vector, Ixodes scapularis. Previous studies have characterized transcriptome proteome response to A. infection. However, postgenomic era, integration of omics datasets through a systems biology approach allows network-based analyses describe complexity functionality biological...
Anaplasma phagocytophilum is an emerging pathogen that causes human granulocytic anaplasmosis. Infection with this zoonotic affects cell function in both vertebrate host and the tick vector, Ixodes scapularis. Global tissue-specific response apoptosis signaling pathways were characterized I. scapularis nymphs adult female midguts salivary glands infected A. using a systems biology approach combining transcriptomics proteomics. Apoptosis was selected for pathway-focused analysis due to its...
Abstract The insect immune deficiency (IMD) pathway resembles the tumour necrosis factor receptor network in mammals and senses diaminopimelic-type peptidoglycans present Gram-negative bacteria. Whether unidentified chemical moieties activate IMD signalling cascade remains unknown. Here, we show that infection-derived lipids 1-palmitoyl-2-oleoyl- sn -glycero-3-phosphoglycerol (POPG) 1-palmitoyl-2-oleoyl diacylglycerol (PODAG) stimulate of ticks. tick protects against colonization by three...
High throughput identification of peptides in databases from tandem mass spectrometry data is a key technique modern proteomics. Common approaches to interpret large scale peptide results are based on the statistical analysis average score distributions, which constructed set best scores produced by collections MS/MS spectra using searching engines such as SEQUEST. Other calculate individual probabilities basis theoretical models or single-spectrum distributions each spectrum. In this work,...
The cattle ticks, Rhipicephalus (Boophilus) spp., affect production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective environmentally friendly alternative to tick control. recombinant microplus Bm86 antigen has been shown protect against infestations. However, variable efficacy Bm86-based geographic strains encouraged research for additional tick-protective antigens. Herein, we describe analysis R. glutathione-S transferase, ubiquitin (UBQ),...
The identification of factors affecting the susceptibility to infectious diseases is essential toward reducing their burden on human population. ABO blood type correlates with malaria and other diseases. Due structural similarity between antigen B Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), we hypothesized that self-tolerance affects immune response α-Gal, which in turn caused by pathogens carrying α-Gal surface. Here found incidence tuberculosis, surface, positively frequency endemic regions....
Ixodid ticks are important vectors of a wide variety viral, bacterial and protozoan pathogens medical veterinary importance. Although several studies have elucidated tick responses to bacteria, little is known about the response viruses. To gain insight into cells flavivirus infection, transcriptomes proteomes two Ixodes spp cell lines infected with tick-borne encephalitis virus (TBEV) were analysed. RNA proteins isolated from scapularis-derived line IDE8 ricinus-derived IRE/CTVM19,...
Epigenetic mechanisms have not been characterized in ticks despite their importance as vectors of human and animal diseases worldwide. The objective this study was to characterize the histones histone modifying enzymes (HMEs) tick vector Ixodes scapularis role during Anaplasma phagocytophilum infection. We first identified 5 34 HMEs I. comparison with similar proteins model organisms. Then, we used transcriptomic proteomic data analyze mRNA protein levels response A. infection tissues...
Abstract The carbohydrate Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) is produced in all mammals except for humans, apes and old world monkeys that lost the ability to synthetize this carbohydrate. Therefore, humans can produce high antibody titers against α-Gal. Anti-α-Gal IgE antibodies have been associated with tick-induced allergy (i.e. α-Gal syndrome) anti-α-Gal IgG/IgM may be involved protection malaria, leishmaniasis Chagas disease. on tick salivary proteins plays an important role etiology of...
Recent technological advances have made multidimensional peptide separation techniques coupled with tandem mass spectrometry the method of choice for high-throughput identification proteins. Due to these advances, development software tools large-scale, fully automated, unambiguous is highly necessary. In this work, we used as a model nuclear proteome from Jurkat cells and present processing algorithm that allows accurate predictions random matching distributions, based on two SEQUEST scores...
We characterized the effects of subolesin and heat shock protein (HSP) expression on Ixodes scapularis Say (Acari: Ixodidae) stress responses to feeding, questing behaviour Anaplasma phagocytophilum (Rickettsiales: Anaplasmataceae) infection. Ticks cultured tick cells were analysed before after subolesin, hsp20 hsp70 gene knock-down by RNA interference. The results these studies confirm that HSPs are involved in cell response both blood-feeding A. Subolesin protective infection may be...
Anaplasma phagocytophilum causes human granulocytic anaplasmosis. Infection with this zoonotic pathogen affects gene expression in both the vertebrate host and tick vector, Ixodes scapularis. Here, we identified new genes, including spectrin alpha chain or alpha-fodrin (CG8) voltage-dependent anion-selective channel mitochondrial porin (T2), that are involved A. infection/multiplication cell response to infection. The downregulated of CG8 salivary glands T2 gut inhibit apoptosis as a...
Tuberculosis (TB) remains a pandemic affecting billions of people worldwide, thus stressing the need for new vaccines. Defining correlates vaccine protection is essential to achieve this goal. In study, we used wild boar model mycobacterial infection and TB characterize protective mechanisms elicited by heat inactivated Mycobacterium bovis (IV). Oral vaccination with IV resulted in significantly lower culture lesion scores, particularly thorax, suggesting that might provide novel control...