A5005936297- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Microbial infections and disease research
- Infectious Diseases and Mycology
- Immune responses and vaccinations
- Immune Response and Inflammation
- Ginseng Biological Effects and Applications
- Zoonotic diseases and public health
- Pharmacological Effects of Natural Compounds
- vaccines and immunoinformatics approaches
- Infectious Diseases and Tuberculosis
- Veterinary medicine and infectious diseases
- Bacteriophages and microbial interactions
- Diagnosis and treatment of tuberculosis
- Humic Substances and Bio-Organic Studies
- Plant Pathogenic Bacteria Studies
- Animal Disease Management and Epidemiology
- Vaccine Coverage and Hesitancy
- Quinazolinone synthesis and applications
- Cancer-related molecular mechanisms research
- Gut microbiota and health
- Microbial Inactivation Methods
- Parasite Biology and Host Interactions
- Antioxidant Activity and Oxidative Stress
- Bird parasitology and diseases
NEIKER, the Basque Institute for Agricultural Research and Development
2016-2025
Euskadiko Parke Teknologikoa
2015-2024
Boeing (Spain)
2023
Servicio Regional de Investigación y Desarrollo Agroalimentario
2023
Tecnalia
2009-2018
Central Institute for Research on Goats
2007
Tuberculosis (TB) caused by Mycobacterium bovis and closely related members of the tuberculosis complex continues to affect humans animals worldwide its control requires vaccination wildlife reservoir species such as Eurasian wild boar (Sus scrofa). Vaccination efforts for TB in have been based primarily on oral live BCG formulations. However, this is first report use inactivated vaccines controlling wildlife. In study, four groups 5 each were vaccinated with M. intramuscular routes, or left...
Mycobacterium avium subspecies paratuberculosis (Map) is an infectious enteric pathogen that causes Johne's disease in livestock. Determining genetic diversity prerequisite to understanding the epidemiology and biology of Map. We performed first whole genome sequencing (WGS) 141 global Map isolates encompass main molecular strain types currently reported. investigated phylogeny strains, limitations commonly used genotyping methods. Single nucleotide polymorphism (SNP) phylogenetic analyses...
Non-tuberculous mycobacteria (NTM) circulate between the environment, animals and humans entailing a double concern: their ability to interfere with tuberculosis diagnosis potential cause infections in hosts. However, published records on NTM are still scarce. The aims of present study were describe diversity circulating among wild domestic species from Spain analyze implications as pathogenic microorganisms or sources interferences bovine tuberculosis. Overall, 293 isolates 277 obtained...
The IS1311 polymerase chain reaction-restriction endonuclease analysis was used to detect genetic differences among 38 Mycobacterium avium subsp. paratuberculosis (Map) isolates from cattle, sheep, goats and bison distinct regions of Spain, India the United States America (USA). In all eight bovine isolates, three out six caprine one ten ovine were C type, while other nine S type. India, five B so (Bison bison) USA. These results show that there are between Map related geographic host...
Johne's disease or paratuberculosis is a chronic granulomatous inflammation of the small intestine ruminants caused by Mycobacterium avium subsp. (MAP). Recent studies suggest an association between MAP and Crohn's in humans. can become widely distributed within tissues infected animals, meat may be possible route exposure to In this study, 47 dairy beef cattle were examined for occurrence viable diaphragm muscle. At slaughterhouse, gut tissues, muscle, blood, feces animals collected...
Rabbits are susceptible to infection by different species of the genus Mycobacterium. Particularly, development specific lesions and isolation Mycobacterium avium subsp. paratuberculosis, both subspecies M. complex, has been reported in wildlife conditions. Although, rabbit meat production worldwide is 200 million tons per year, microbiological data on this source lacking more specifically reports mycobacterial presence industrially reared for human consumption have not published. To end, we...
Tuberculosis (TB) remains a pandemic affecting billions of people worldwide, thus stressing the need for new vaccines. Defining correlates vaccine protection is essential to achieve this goal. In study, we used wild boar model mycobacterial infection and TB characterize protective mechanisms elicited by heat inactivated Mycobacterium bovis (IV). Oral vaccination with IV resulted in significantly lower culture lesion scores, particularly thorax, suggesting that might provide novel control...
ABSTRACT Mycobacterium tuberculosis complex, avium , and many other nontuberculous mycobacteria are worldwide distributed microorganisms of major medical veterinary importance. Considering the growing epidemiologic significance wildlife-livestock-human interrelation, developing rapid detection tools high specificity sensitivity is vital to assess their presence accelerate process diagnosing mycobacteriosis. Here we describe development evaluation a novel tetraplex real-time PCR for...
Tuberculosis (TB) in goats is a chronic infectious disease caused by Mycobacterium tuberculosis complex (MTBC) organisms that pose great health and economic challenge for the caprine industry some European developing countries. It also zoonotic posing risk public health. The control programs of are based on test-and-slaughter strategy, vaccination not feasible with available vaccines due to its interferences current TB immunodiagnosis. There still need development an effective vaccine and,...
<title>Abstract</title> <bold>Background</bold>: Bovine tuberculosis (TB) caused by <italic>Mycobacterium bovis</italic> remains a persistent zoonotic and agricultural challenge, despite advances in eradication programs targeting cattle. The complexity of TB epidemiology, especially extensive livestock systems wildlife reservoirs, necessitates novel control strategies. Vaccination has re-emerged as promising tool, with growing interest inactivated vaccines for both efficacy diagnostic...
Abstract Background Mycobacterium avium subsp. paratuberculosis ( Map ) causes in animals and is suspected of causing Crohn's Disease humans. Characterization strains led to classify isolates two main types, cattle type strains, found affecting all host species, sheep reported mainly sheep. In order get a better understanding the epidemiology large set obtained from different species over last 25 years have been characterized. Five-hundred twenty hosts (cattle, sheep, goats, bison, deer wild...
Similarities between human inflammatory bowel disease (IBD) and ruminant paratuberculosis have fueled a heated discussion on the role of Mycobacterium avium subsp. (MAP) in etiology IBD.In order to determine microbiological immunological evidence an association MAP IBD, blood from 222 patients 80 healthy donors Basque Country (Spain) were subjected nested PCR for MAP-specific insertion sequence IS900, interferon-gamma (IFN-gamma) release test with PPA-3 antigen (IFNMAP) or phosphate-buffered...
BackgroundMycobacteria, such as M. leprae and tuberculosis infect billions of humans. However, because appropriate immune responses antibiotic therapy, overt mycobacterial diseases occur far less frequently. avium subspecies paratuberculosis (MAP) causes Johne's disease in ruminants, an affliction evocative inflammatory bowel (IBD). Several agents used to treat IBD (5-ASA, methotrexate, azathioprine its metabolite 6-MP) have recently been shown be antiMAP antibiotics. We herein evaluate the...
Paratuberculosis vaccination has been in use some regions for many decades, but results have not widely spread. A new Mycobacterium avium subsp. paratuberculosis (MAP) killed vaccine was studied relationship with its effects on fecal shedding and milk production four farms while other two were kept as controls submitted to a test cull scheme. Fecal detection (n = 1829) milking records 2413) analyzed after (5 herds) (1 herd) years of the beginning intervention. Shedder prevalence reduced by...
ABSTRACT Mycobacterium avium subsp. paratuberculosis causes (Johne's disease) in ruminants most countries. Historical data suggest substantial differences culturability of M. isolates from small and cattle; however, a systematic comparison culture media different countries hosts has not been undertaken. Here, 35 field the United States, Spain, Northern Ireland, Australia were propagated Bactec 12B medium Middlebrook 7H10 agar, genomically characterized, subcultured to Lowenstein-Jensen (LJ),...
Mycobacterium avium subspecies paratuberculosis (Map) is the aetiological agent of Johne's disease or and included within complex (MAC). Map strains are two major types often referred to as 'Sheep' 'S-type' 'Cattle' 'C-type'. With advent more discriminatory typing techniques it has been possible further classify S-type into groups Type I III. This study was undertaken genotype a large panel small ruminant isolates from different hosts geographical origins compare them with well documented...
ABSTRACT The enteropathy called paratuberculosis (PTB), which mainly affects ruminants and has a worldwide distribution, is caused by Mycobacterium avium subsp. . This disease significantly reduces the cost-effectiveness of ruminant farms, therefore, reliable rapid detection methods are needed to control spread bacterium in livestock environment. aim this study was identify specific sensitive combination DNA extraction amplification detect M. feces. Negative bovine fecal samples were...
Abstract The single and comparative intradermal tuberculin tests (SITT CITT) are official in vivo for bovine tuberculosis (TB) diagnosis using avian purified protein derivatives (PPD-B PPD-A). Infection with bacteria other than Mycobacterium complex (MTC) can result nonspecific reactions to these tests. We evaluated the performance of skin test PPDs new defined antigens guinea pig model. A standard dose (SD) Rhodococcus equi , Nocardia sp., M . nonchromogenicum monacense intracellulare avium...
The growth rate of Mycobacterium avium subsp. paratuberculosis was assessed by different methods in 7H9 medium supplemented with OADC (oleic acid, albumin, dextrose, catalase), Tween 80, and mycobactin J. Generation times maximum specific rates were determined wet weight, turbidometric measurement, viable count, quantitative PCR (ParaTB-Kuanti; F57 gene) for 8 M. strains (K10, 2E, 316F, 81, 445, 764, 22G, OVICAP 49). Strain-to-strain differences observed curves calculated parameters....
Although there is a wide consensus on the efficacy of paratuberculosis vaccination to limit economic losses, its use has been restricted because interference in diagnosis tuberculosis. Data from vaccine clinical trial Basque Country (Spain) evaluated relationship with bovine tuberculosis intradermal test results. The included two herds applying Test and Culling strategy five an inactivated vaccine. was applied animals all ages present each vaccinated herd when joining trial, then replacers...