A5055741412- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Microbial infections and disease research
- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Gut microbiota and health
- Infectious Diseases and Mycology
- Herpesvirus Infections and Treatments
- Animal Disease Management and Epidemiology
- Immune Response and Inflammation
- Helminth infection and control
- Reproductive System and Pregnancy
- HIV Research and Treatment
- Pharmacological Effects of Natural Compounds
- Sex work and related issues
- Inflammatory Bowel Disease
- RNA regulation and disease
- Veterinary Oncology Research
- Animal Nutrition and Physiology
- Vector-Borne Animal Diseases
- Immune responses and vaccinations
- Mycotoxins in Agriculture and Food
- Skin and Cellular Biology Research
- Forensic Entomology and Diptera Studies
- Coccidia and coccidiosis research
Universidad de León
2012-2025
Euskadiko Parke Teknologikoa
2017-2025
Universidad de León
2025
NEIKER, the Basque Institute for Agricultural Research and Development
2018-2024
University of Antwerp
2023-2024
Instituto de Investigación Biomédica de Salamanca
2024
Centro de Investigación Biomédica en Red de Cáncer
2024
Hospital Universitari i Politècnic La Fe
2023
KU Leuven
2023
Instituto de Ganadería de Montaña
2012-2022
Neospora caninum has been detected only sporadically in cases of ovine abortion, and it therefore traditionally considered as an unimportant parasite small ruminants. This study was carried out with the aim identifying pathogen causing serious reproductive problems on a commercial sheep farm. Sera from all rams ewes tested negative for antibodies against Border disease virus, Schmallenberg virus Coxiella burnetii, infections by these agents were ruled out. Nevertheless, seropositivity to N....
Animals infected with Mycobacterium avium subspecies paratuberculosis show a variety of granulomatous lesions that range from focal forms, seen in the subclinical stages, to diffuse associated clinical signs. The aim this study was phenotypically characterize macrophages present different lesion types using immunohistochemical methods. Lesions total 23 animals bovine paratuberculosis, natural and experimental, were examined by immunohistochemistry. Antibodies against inducible nitric oxide...
Abstract Recent evidence demonstrates potential links between mitochondrial dysfunction and inflammatory bowel diseases (IBD). In addition, bidirectional interactions the intestinal microbiota host mitochondria may modulate inflammation. We observed previously that mice deficient in protein MCJ (Methylation-controlled J protein) exhibit increased susceptibility to DSS colitis. However, it is unclear whether this phenotype primarily driven by −/− associated gut dysbiosis or direct effects of...
Recent studies hint at mitochondrial genes influencing UC patient response to anti-TNF treatment. We evaluated this hypothesis by following a targeted strategy identify gene expression that captures the relationship between dysregulation and Our objective was initially examine in colon samples subsequently assess whether resulting signal persists bloodstream. analyzed transcriptome of from an anti-TNF-treated murine model characterized impaired activity treatment resistance. then transferred...
<title>Abstract</title> <bold>Background</bold>: Bovine tuberculosis (TB) caused by <italic>Mycobacterium bovis</italic> remains a persistent zoonotic and agricultural challenge, despite advances in eradication programs targeting cattle. The complexity of TB epidemiology, especially extensive livestock systems wildlife reservoirs, necessitates novel control strategies. Vaccination has re-emerged as promising tool, with growing interest inactivated vaccines for both efficacy diagnostic...
After oral administration of ewes during mid gestation with 2000 freshly prepared sporulated oocysts T. gondii isolate M4, abortions occurred between days 7 and 11 in 91.6% pregnant infected ewes. Afterwards, a further infection was carried out at late another group sheep 500 oocysts. Abortions happened again 9 post (pi) 58.3% the Classically, natural experimental ovine toxoplasmosis usually occur one month after infection. Few studies have reported so-called acute phase as early to 14...
Neospora caninum is considered one of the main causes abortion in cattle, yet recent studies have also emphasised its relevance as an abortifacient small ruminants. In order to gain deeper insight into pathogenesis ovine neosporosis, pregnant ewes were intravenously inoculated with 106 tachyzoites Nc-Spain7 isolate at days 40, 90 or 120 gestation. Infection during first term resulted death all foetuses between 19 and 21 post-infection, showing mainly necrotic lesions foetal liver highest...
Abstract The single and comparative intradermal tuberculin tests (SITT CITT) are official in vivo for bovine tuberculosis (TB) diagnosis using avian purified protein derivatives (PPD-B PPD-A). Infection with bacteria other than Mycobacterium complex (MTC) can result nonspecific reactions to these tests. We evaluated the performance of skin test PPDs new defined antigens guinea pig model. A standard dose (SD) Rhodococcus equi , Nocardia sp., M . nonchromogenicum monacense intracellulare avium...
The two main genotypes of recognized isolates Mycobacterium avium subsp. paratuberculosis (Map) are cattle (C) and sheep (S) strains. An experimental infection was conducted to establish the effect Map strain on pathogenesis ovine paratuberculosis. Twenty-four out thirty 1.5-month-old Assaf lambs were divided into 4 groups 6 infected orally with three low passage field isolates, S- (22G pigmented Ovicap49) one C– (764) type, reference K-10 (C type). remaining six animals unchallenged...
The relation between gestational age and foetal death risk in ovine toxoplasmosis is already known, but the mechanisms involved are not yet clear. In order to study how stage of gestation influences these mechanisms, pregnant sheep same genetic background were orally dosed with 50 oocysts Toxoplasma gondii (M4 isolate) at days 40 (G1), 90 (G2) 120 (G3) gestation. each group, four animals culled on second, third fourth week post infection (pi) evaluate parasite load distribution, lesions...
Whole genome sequencing (WGS) holds great potential for the management and control of tuberculosis. Accurate analysis samples with low mycobacterial burden, which are characterized by (<20x) coverage high (>40%) levels contamination, is challenging. We created MAGMA (Maximum Accessible Genome Mtb Analysis) bioinformatics pipeline clinical samples.High accuracy variant calling achieved using a long seedlength during read mapping to filter out contaminants, quality score recalibration machine...
Mycobacterium avium subsp paratuberculosis ( Map) is assumed to infect young ruminants; however, little known concerning the possibility of adult animals becoming infected. An experimental infection was conducted establish effect age and doses Map on susceptibility in sheep. Sixteen twenty-four 1.5-month-old Churra lambs 23 30 ewes (from 2–11 years old) were orally challenged with an ovine field strain Map. Thirteen 8 infected a high dose (HD) 10 sheep low (LD) The remaining unchallenged...
Mycobacterium avium subsp. paratuberculosis (Map) is the etiological agent of (PTB), a chronic intestinal inflammatory disease that causes high economical losses in dairy livestock worldwide. Due to absence widely available preventive or therapeutical treatments, new alternative therapies are needed. In this study, effect probiotic alone combination with commercial vaccine has been evaluated rabbit model. Vaccination enhanced humoral response, exerted training peripheral polymorphonuclear...
Between 1997 and March 2004, the nervous form, or visna, of maedi-visna infection was diagnosed in 71 1631 sheep (4.35 per cent) examined Castilla y León region Spain, which 634 had shown signs. The presence virus confirmed by immunohistochemistry some cases pcr on frozen-thawed paraffin-embedded tissue samples. main clinical signs were hindleg ataxia paresis, but blindness nystagmus also observed. Thirty-three affected (46.5 two years old younger. showed variable degrees a non-suppurative...
A case of unilateral suppurative epididymo-orchitis associated with Salmonella enterica subsp. diarizonae serovar 61:k:1,5,(7) infection is described in a 2-year-old ram. Gross lesions were characterized by severe enlargement the scrotal contents, fibrous adhesions between testicular layers, coexistence epididymal abscesses and foci fibrinous exudate, atrophy. Microscopically, microabscesses diffuse inflammatory infiltrates abundant macrophages containing short Gram-negative rods observed....
Abstract Although it is known that gestation could influence the clinical course of ovine toxoplasmosis, precise effect term when sheep are infected yet mostly unknown. The aim this study was to evaluate peripheral and placental immune responses developed in pregnant after experimental infection with Toxoplasma gondii at different times gestation. Thirty-six were allocated groups, orally inoculated sporulated oocysts T. early, mid late culled within 30 days post-infection. humoral cytokine...
Animal tuberculosis (TB) remains a major problem in some countries despite the existence of control programmes focused mainly on cattle. In this species, aerogenous transmission is accepted as most frequent infection route, affecting respiratory system. Under hypothesis that oral route could be playing more relevant role transmission, diagnosis and disease persistence than previously thought, study was performed to assess course TB cattle its effects depending entry Mycobacterium bovis. Two...
Anti-TNF therapy can induce and maintain a remission status during intestinal bowel disease. However, up to 30% of patients do not respond this by mechanisms that are unknown. Here, we show the absence MCJ, natural inhibitor respiratory chain Complex I, induces gut microbiota changes critical determinants lack response in murine model DSS-induced inflammation. First, found MCJ expression is restricted macrophages human colonic tissue. Therefore, demonstrate transcriptomic analysis colon from...