A5080062095- Vector-borne infectious diseases
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Aquaculture Nutrition and Growth
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Plant Virus Research Studies
- Veterinary Medicine and Surgery
- Insect and Arachnid Ecology and Behavior
- Insect Resistance and Genetics
- Neuroblastoma Research and Treatments
- Animal Genetics and Reproduction
- Neurobiology and Insect Physiology Research
- Veterinary Orthopedics and Neurology
- Meat and Animal Product Quality
- Natural Antidiabetic Agents Studies
- Fatty Acid Research and Health
- Aquaculture disease management and microbiota
- Reproductive biology and impacts on aquatic species
- Wnt/β-catenin signaling in development and cancer
- Virus-based gene therapy research
- Sperm and Testicular Function
- Plant Parasitism and Resistance
- Advanced Glycation End Products research
- Veterinary Oncology Research
Czech Academy of Sciences, Biology Centre
2020-2024
Institute of Parasitology
2020-2024
National Institute of Allergy and Infectious Diseases
2022-2024
Vector (United States)
2022-2024
National Institutes of Health
2022-2023
Central European Institute of Technology
2020-2022
Universidade Federal do Rio de Janeiro
2016-2022
Czech Academy of Sciences
2022
University of Veterinary Sciences Brno
2020
Universidade Federal do Rio Grande do Sul
2010-2014
Abstract The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor symbiont bacterium the genus Coxiella . It was showed endosymbiont from R (CERM) vertically transmitted mutualist symbiont, comprising 98% 16S rRNA sequences both eggs and larvae. Sequencing bacterial genome revealed genes for biosynthetic pathways several vitamins key metabolic cofactors may provide nutritional complement to...
Culture-independent metagenomic methodologies have enabled detection and identification of microorganisms in various biological systems often revealed complex unknown microbiomes. In many organisms, the microbiome outnumbers host cells greatly affects biology fitness. Ticks are hematophagous ectoparasites with a wide range. They vector number human animal pathogens also directly cause major economic losses livestock. Although several reports on tick midgut microbiota show diverse bacterial...
The tick Rhipicephalus (Boophilus) microplus is a blood-sucking ectoparasite of cattle that severely impairs livestock production. Studies on immunological control address mostly single-antigen vaccines. However, from the commercial standpoint, so far no vaccine has afforded appropriate protection against all R. populations. In this context, multi-antigen cocktails have emerged as way to enhance efficacy. work, multi-antigenic was analyzed under field conditions in naturally infested cattle....
Ticks have serious impacts on animal and human health, causing significant economic losses in cattle breeding. Besides damage due to the hematophagous behavior, they transmit several pathogens. Low cost environmental safety made vaccines a promising alternative control method against tick infestation. Metalloproteases (MPs) been shown be essential for diverse biological functions organisms, inhibiting blood clotting, degrading extracellular matrix proteins, host tissue repair via...
In addition to being vectors of pathogenic bacteria, ticks also harbor intracellular bacteria that associate with over generations, aka symbionts. The biological significance such bacterial symbiosis has been described in several tick species but its function Ixodes ricinus is not understood. We have previously shown I. are primarily inhabited by a single symbiont, Midichloria mitochondrii, an bacterium resides and reproduces mainly the mitochondria ovaries fully engorged females. To study...
Artificial membrane feeding (AMF) is a powerful and versatile technique with wide range of applications in the study disease vectors species. Since its first description, AMF has been under constant optimization standardization for different tick species life stages. In USA, Ixodes scapularis main vector tick-borne zoonoses including pathogens causing Lyme humans animals. Seeking to improve overall fitness I. adult females fed artificially, here, we have optimized technique, considerably...
Abstract To further obtain insights into the Rhipicephalus microplus transcriptome, we used RNA-seq to carry out a study of expression in (i) embryos; (ii) ovaries from partially and fully engorged females; (iii) salivary glands (iv) fat body (v) digestive cells partially, (vi) females. We obtained > 500 million Illumina reads which were assembled de novo, producing 190,000 contigs, identifying 18,857 coding sequences (CDS). Reads each library mapped back transcriptome giving view gene...
In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional showed that obligate endosymbionts contribute to biology, affecting reproductive fitness molting. Understanding molecular basis of interaction ticks their mutualist may help develop control methods based on microbiome manipulation. Previously, we Rhipicephalus microplus larvae with reduced levels Coxiella...
Ticks are obligate hematophagous arthropods transmitting a wide range of pathogens to humans and animals. They also harbor non-pathogenic microbiota, primarily in the ovaries midgut. In previous study on Ixodes ricinus, we used culture-independent approach showed diverse but quantitatively poor midgut bacterial microbiome. Our analysis revealed absence core microbiome, suggesting an environmental origin tick microbiota.A adult females collected by flagging from two localities Czech Republic...
SUMMARY Glycogen synthase kinase-3 (GSK-3) is classically described as a key enzyme involved in glycogen metabolism mammals. It has been shown to be highly conserved among several organisms, mainly the catalytic domain region. This already Rhipicephalus (Boophilus) microplus and ovaries of females appeared major site GSK-3 transcription. The treatment with specific inhibitor (alsterpaullone, bromo-indirubin-oxime 6 indirubin-3-oxime) caused reduction oviposition egg hatching completely...
Ticks are obligate hematophagous arthropods that transmit a wide range of pathogens to humans as well wild and domestic animals. They also harbor non-pathogenic microbiota, although our previous study has shown the diverse bacterial microbiome in midgut Ixodes ricinus is quantitatively poor lacks core. In artificial infections by capillary feeding ticks with two model bacteria (Gram-positive Micrococcus luteus Gram-negative Pantoea sp.), rapid clearance these microbes from was observed,...
The Gulf Coast tick, Amblyomma maculatum, is a vector of several tick-borne pathogens, including Rickettsia parkeri. ability R. parkeri to persist within the tick population through transovarial and transstadial transmission, without apparently harming ticks, contributes pathogen's perpetuation in population. Previous studies have shown that load A. maculatum regulated by tissues' oxidant/antioxidant balance non-pathogenic microbiome. To obtain further insights into interaction between...
Tick saliva helps blood feeding by its antihemostatic and immunomodulatory activities. salivary gland transcriptomes (sialotranscriptomes) revealed thousands of transcripts coding for putative secreted polypeptides. Hundreds these code groups similar proteins, constituting protein families, such as the lipocalins metalloproteases. However, while many transcriptome-derived sequences matches predicted tick genome assemblies, majority are not represented in proteomes. The diversity could derive...