A5074474672- Vector-borne infectious diseases
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Viral Infections and Vectors
- Toxin Mechanisms and Immunotoxins
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Plant pathogens and resistance mechanisms
- Food Allergy and Anaphylaxis Research
- Helminth infection and control
- Parasite Biology and Host Interactions
- Invertebrate Immune Response Mechanisms
- Insect Pest Control Strategies
- Herpesvirus Infections and Treatments
- Insect Resistance and Genetics
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Entomopathogenic Microorganisms in Pest Control
- Enzyme Production and Characterization
- Insect and Arachnid Ecology and Behavior
- Parasites and Host Interactions
- Protein Hydrolysis and Bioactive Peptides
- Neurobiology and Insect Physiology Research
- Phytase and its Applications
- Mast cells and histamine
- Antimicrobial Peptides and Activities
National Institute of Allergy and Infectious Diseases
2019-2024
Universidade Federal do Rio Grande do Sul
2013-2024
Abdul Wali Khan University Mardan
2024
Universidade Federal do Rio de Janeiro
2024
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
2024
National University of Singapore
2024
National Institutes of Health
2020-2023
Texas A&M University
2014-2022
Mitchell Institute
2019
Vector (United States)
2019
The cattle tick Rhipicephalus (Boophilus) microplus is one of the most harmful parasites affecting bovines. Similarly to other hematophagous ectoparasites, R. saliva contains a collection bioactive compounds that inhibit host defenses against feeding activity. Thus, study salivary components offers opportunities for development immunological based control methods and medicinal applications. So far, only few proteins have been identified in saliva. aim this work was identify present female at...
Abstract The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor symbiont bacterium the genus Coxiella . It was showed endosymbiont from R (CERM) vertically transmitted mutualist symbiont, comprising 98% 16S rRNA sequences both eggs and larvae. Sequencing bacterial genome revealed genes for biosynthetic pathways several vitamins key metabolic cofactors may provide nutritional complement to...
Ixodes scapularis is the most medically important tick species and transmits five of 14 reportable human borne disease (TBD) agents in USA. This study describes LC-MS/MS identification 582 tick- 83 rabbit proteins saliva I. ticks that fed for 24, 48, 72, 96, 120 h, as well engorged but not detached (BD), spontaneously (SD). The include proteases (5.7%), protease inhibitors (7.4%), unknown function (22%), immunity/antimicrobial (2.6%), lipocalin (3.1%), heme/iron binding extracellular matrix/...
Lyme disease (LD) caused by Borrelia burgdorferi is among the most important human vector borne diseases for which there no effective prevention method. Identification of tick saliva transmission factors LD agent needed before highly advocated antigen-based vaccine could be developed. We previously reported conserved Ixodes scapularis ( Ixs ) serpin (S) 17 S17) was secreted B . infected nymphs. Here, we show that S17 promote feeding and enhances colonization host. not part a redundant...
Haemaphysalis longicornis is a major vector of Theileria spp., Anaplasma phagocytophilum, Babesia spp. and Coxiella burnetti in East Asian countries. All life stages ixodid ticks have destructive pool-feeding style which they create site by lacerating host tissue secreting variety biologically active compounds that allows the tick to evade responses, enabling uptake blood meal. The identification functional characterization saliva proteins can be useful elucidate molecular mechanisms...
Understanding the molecular basis of how ticks adapt to feed on different animal hosts is central understanding tick and tick-borne disease (TBD) epidemiology. There evidence that differentially express specific sets genes when stimulated start feeding. This study was initiated investigate if such as Ixodes scapularis Amblyomma americanum are adapted multiple utilized same proteins prepare for We exposed I. A. feeding stimuli (rabbit, human, dog) by keeping unfed adult enclosed in a...
Abstract Fasciola hepatica is the agent of fasciolosis, a foodborne zoonosis that affects livestock production and human health. Although flukicidal drugs are available, re-infection expanding resistance to triclabendazole demand new control strategies. Understanding molecular mechanisms underlying complex interaction with mammalian host could provide relevant clues, aiding search for novel targets in diagnosis fasciolosis. Parasite survival mediated by parasite compounds released during...
Abstract Background When feeding on a vertebrate host, ticks secrete saliva, which is complex mixture of proteins, lipids, and other molecules. Tick saliva assists the vector in modulating host hemostasis, immunity, tissue repair mechanisms. While helping to feed, its modifies site where pathogens are inoculated often facilitates infection process. The objective this study uncover variation protein composition Rhipicephalus microplus during blood feeding. Methods Ticks were fed calves, adult...
Ticks have serious impacts on animal and human health, causing significant economic losses in cattle breeding. Besides damage due to the hematophagous behavior, they transmit several pathogens. Low cost environmental safety made vaccines a promising alternative control method against tick infestation. Metalloproteases (MPs) been shown be essential for diverse biological functions organisms, inhibiting blood clotting, degrading extracellular matrix proteins, host tissue repair via...
Amblyomma americanum ticks transmit more than a third of human tick-borne disease (TBD) agents in the United States. Tick saliva proteins are critical to success as vectors TBD agents, and thus might serve targets tick antigen-based vaccines prevent infections. We describe systems biology approach identify, by LC-MS/MS, (tick = 1182, rabbit 335) that A. likely inject into host every 24 h during first 8 days feeding, towards end feeding. Searching against entries GenBank grouped 27 25...
Serpins are widely distributed and functionally diverse inhibitors of serine proteases. Ticks secrete serpins with anti-coagulation, anti-inflammatory, immunomodulatory activities via their saliva into the feeding cavity to modulate host’s hemostatic immune reaction initiated by insertion tick’s mouthparts skin. The suppression response not only allows ticks feed on a host for several days but also creates favorable conditions transmission tick-borne pathogens. Herein we present functional...
Proteins belonging to the serine protease inhibitor (serpin) superfamily play essential roles in many organisms. In arthropods these proteins are involved innate immune system, morphogenesis and development. mammals serpins regulate pathways that life such as blood coagulation, fibrinolysis, inflammation complement activation, some of which considered host's first line defense hematophagous and/or dueling parasites. Thus, it is hypothesized ticks use evade host defense, facilitating...
Abstract Ticks are arthropod ectoparasites of importance for public and veterinary health. The understanding tick oogenesis embryogenesis could contribute to the development novel control methods. However, date, studies on temporal dynamics proteins during ovary were not reported. In present study we followed protein profile maturation. Proteomic analysis extracts was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using shotgun strategy, in addition dimethyl...
Abstract To further obtain insights into the Rhipicephalus microplus transcriptome, we used RNA-seq to carry out a study of expression in (i) embryos; (ii) ovaries from partially and fully engorged females; (iii) salivary glands (iv) fat body (v) digestive cells partially, (vi) females. We obtained > 500 million Illumina reads which were assembled de novo, producing 190,000 contigs, identifying 18,857 coding sequences (CDS). Reads each library mapped back transcriptome giving view gene...
In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional showed that obligate endosymbionts contribute to biology, affecting reproductive fitness molting. Understanding molecular basis of interaction ticks their mutualist may help develop control methods based on microbiome manipulation. Previously, we Rhipicephalus microplus larvae with reduced levels Coxiella...