Haemaphysalis longicornis is a major vector of Theileria spp., Anaplasma phagocytophilum, Babesia spp. and Coxiella burnetti in East Asian countries. All life stages ixodid ticks have destructive pool-feeding style which they create site by lacerating host tissue secreting variety biologically active compounds that allows the tick to evade responses, enabling uptake blood meal. The identification functional characterization saliva proteins can be useful elucidate molecular mechanisms...

A cDNA encoding tick chitinase was cloned from a library of mRNA Haemaphysalis longicornis eggs and designated as CHT1 cDNA. The contains an open reading frame 2790 bp that codes for 930 amino acid residues with coding capacity 104 kDa. deduced sequence shows 31% homology to Aedes aegypti multidomain structure containing one chitin binding peritrophin domain two glycosyl hydrolase family 18 domains. endogenous H. identified by two-dimensional immunoblot analysis mouse anti-rCHT1 serum shown...

Abstract Severe fever with thrombocytopenia syndrome (SFTS) is an emerging zoonotic disease, which causes high fever, thrombocytopenia, and death in humans animals East Asian countries. The pathogenicity of SFTS virus (SFTSV) remains unclear. We intraperitoneally infected three groups mice: wild-type (WT), mice treated blocking anti-type I interferon (IFN)-α receptor antibody (IFNAR Ab), IFNAR knockout −/− ) mice, four doses SFTSV (KH1, 5 × 10 to 2 FAID 50 ). WT survived all infective doses....

Ticks, blood-sucking ectoparasites, spread diseases to humans and animals. <i>Haemaphysalis longicornis</i> is a significant vector for tick-borne in medical veterinary contexts. Identifying protective antigens <i>H. an anti-tick vaccine key tick control strategy. Enolase, multifunctional protein, significantly converts D-2-phosphoglycerate phosphoenolpyruvate glycolysis gluconeogenesis cell cytoplasm. This study cloned complete open reading frame (ORF) of enolase from the...

A 3-yr-old female mongrel dog was referred to the Veterinary Teaching Hospital of Chungnam National University in Republic Korea. An adult heartworm, Dirofilaria immitis, found abdominal cavity during spaying. Dirofilariasis this also diagnosed by modified Knott's test, ELISA and PCR analysis. The present case is first report on migration an heartworm a Key words: dog, migration,

The success of immunological method for the control ticks depend on use potential key antigens as tick vaccine candidates. Chitinase is induced by ecdysteroids to degrade older chitin at time molting. Previously, we cloned a gene encoding 113 kDa protein (CHT1) Haemaphysalis longicornis, and identified CHT1 chitinase (You et al. 2003). In this study, recombinant (rCHT1) expressed in Escherichia coli was used immunize mice. mice were challenge-infested with different developmental stages same...

Background:Clostridium tertium is distributed in the soil and animal human gastrointestinal tracts. C. has been isolated from patients with blood diseases, immune disorders, abdominal surgeries. Glyphosate toxic, causing cause eye skin irritation, pain, vomiting. Ingestion of herbicides modifies environment, which stresses living organisms. However, there little attention to cases bacteremia recovering suicide attempt by ingesting herbicide. Case Report:Clostridium was identified a...

Tick infestation causes a significant threat to human and animal health, requiring effective immunological control methods. This study aimed investigate the potential of recombinant Haemaphysalis longicornis enolase protein for tick vaccine development. The exact mechanism recently identified from H. Jeju strain remains poorly understood. Enolase plays crucial role in glycolysis, metabolic process that converts glucose into energy, is essential motility, adhesion, invasion, growth,...

Longicin, a defensin-like peptide, was recently identified in the hard tick Haemaphysalis longicornis. Longicin and one of its synthetic partial analogs (P4) displayed antimicrobial/fungicidal/parasiticidal activity. In present study, we compared longicin-derived order to characterize antimicrobial motif by analyzing some structural features using various bioinformatic tools and/or CD spectroscopy. According chemicophysical characteristics, P4 is suggested be cationic peptide with...

Glycogen synthase kinase 3 (GSK-3), which belongs to the serine/threonine family, regulates glycogen metabolism, Wnt signaling, hormonal regulation, and embryonic development in many eukaryotes. Here, we cloned a complete open reading frame (ORF) of 3β (GSK-3β) from Haemaphysalis longicornis characterized its transcriptional functional status. The ORF GSK-3β possesses 1242 nucleotides encoding mature protein 413 amino acid residues. nucleotide sequences are highly conserved among different...

Background/Aim: Acute liver injury (ALI) is a life-threatening clinical syndrome that usually caused by toxic chemicals, drugs, or pathogen infections. Sirtuin2 (Sirt2), an NAD+-dependent deacetylase, appears to play detrimental roles in injury. Here, we evaluated the therapeutic application targeting Sirt2 carbon tetrachloride (CCl4)-induced ALI, using AK-1 (a inhibitor).Methods: For vivo experiments, single injection of CCl4 was used induce ALI. One hour later, mice were intraperitoneally...

is an obligate blood-sucking ectoparasite that has gained attention due its role of transmitting medically and veterinary significant pathogens it the most common tick species in Republic Korea. The preferred strategy for controlling ticks a multi-antigenic vaccination. Testing efficiency combination antigen promising method creating vaccine.

This study evaluated the potential repellent and acaricidal effects of 4 essential oils (clove, eucalyptus, lavender, mint) against Asian longhorned tick <i>Haemaphysalis longicornis</i>, a vector various tick-borne diseases in medical veterinary contexts. Selected for their properties, were tested on adult nymph <i>H. longicornis</i> ticks at different concentrations. The experiment assessed mortality rates repellency, particularly during attachment to host skin....

Currently, multi-antigenic vaccine use is the method of choice for strategic control ticks. Therefore, determining efficacy combined antigens a promising avenue research in development anti-tick vaccines. The antigen responsible blood intake and reproduction has proven suitable as antigen. It been shown to silence Haemaphysalis longicornis salivary cystatin (HlSC-1) subolesin by RNA interference. Adult unfed female ticks were injected with double-stranded (A) subolesin, (B) cystatin, (C)...

We investigated the induction of resistance to Haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant H. P27/30 protein. The success immunological control methods is dependent upon use potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 proteins (P27/30) longicornis, identified troponin I-like In this study, were expressed Escherichia coli showed statistically significant longer feeding duration for larval...

Proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. For detection antigens from Haemaphysalis longicornis, 1-dimensional electrophoresis (1-DE) quantitative immunoblotting technique combined with 2-dimensional (2-DE) was used whole body proteins unfed partially fed female ticks. Reactivity bands 2-DE were performed following to identify protein spots. The proteome had a larger number lower molecular weight...

We reported an outbreak of Aeromonas (A.) veronii responsible for ulcerative dermatitis in Israeli carp (Cyprinus carpio). The major clinical signs were darkening body, abdominal extension, exophthalmos and severe necrosis the skin. necropsy showed yellowish ascites, liver enlargement kidney spleen morbid fish. In blood agar culturing bacteria, three different colonies identified as A. veronii, Plesimonas shigelloides Shewanella putrefaciens by phylogenetic identification using 16S rRNA or...

Various chemotherapeutic agents have been used largely for the treatment of salivary gland cancer. However, results are disappointing, and these can cause some serious side effects. Therefore, recent studies focused on possible roles natural products to overcome limitations.Salivary cancer cells treated with or without Convallaria keiskei (MECK) 24 hours. Apoptotic changes were evaluated by live/dead assay, immunoblotting, expression levels caspase-3 B-cell lymphoma-2 family member.MECK...