A5091549922- Vector-borne infectious diseases
- Insect and Pesticide Research
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Study of Mite Species
- Mosquito-borne diseases and control
- Bird parasitology and diseases
- Toxin Mechanisms and Immunotoxins
- Malaria Research and Control
- Iron Metabolism and Disorders
- Neurobiology and Insect Physiology Research
- Insect-Plant Interactions and Control
- Invertebrate Immune Response Mechanisms
- Insect Pest Control Strategies
- Plant Virus Research Studies
- Drug Transport and Resistance Mechanisms
- Entomological Studies and Ecology
- Hemoglobin structure and function
- Structural Engineering and Materials Analysis
- Glutathione Transferases and Polymorphisms
- Plant Parasitism and Resistance
- Parasitic Diseases Research and Treatment
- Trypanosoma species research and implications
- Parasites and Host Interactions
Czech Academy of Sciences, Biology Centre
2015-2024
Czech Academy of Sciences
2014-2024
Institute of Parasitology
2015-2024
University of South Bohemia in České Budějovice
2015-2018
Sewanee: The University of the South
2013-2016
Wellcome Centre for Molecular Parasitology
2011
Wellcome Trust
2011
University of Glasgow
2011
Haem and iron homeostasis in most eukaryotic cells is based on a balanced flux between haem biosynthesis oxygenase-mediated degradation. Unlike eukaryotes, ticks possess an incomplete biosynthetic pathway and, together with other (non-haematophagous) mites, lack gene encoding oxygenase. We demonstrated, by membrane feeding, that do not acquire bioavailable from haemoglobin-derived haem. However, require dietary haemoglobin as exogenous source of since, feeding haemoglobin-depleted serum led...
Abstract Adult females of the genus Ixodes imbibe blood meals exceeding about 100 times their own weight within 7‒9 days. During this period, ticks internalise components host by endocytic digest cells that line tick midgut epithelium. Using RNA-seq, we aimed to characterise transcriptome composition in adult ricinus during early and late phase engorgement. To address specific adaptations haemoglobin-rich diet, compared transcriptomes genetically homogenous female siblings fed either bovine...
Ticks salivate while feeding on their hosts. Saliva helps blood through host anti-hemostatic and immunomodulatory components. Previous transcriptomic proteomic studies revealed the complexity of tick saliva, comprising hundreds polypeptides grouped in several multi-genic families such as lipocalins, Kunitz-domain containing peptides, metalloproteases, basic tail secreted proteins, other uniquely found ticks. These also that composition saliva changes with time; expression transcripts from...
Culture-independent metagenomic methodologies have enabled detection and identification of microorganisms in various biological systems often revealed complex unknown microbiomes. In many organisms, the microbiome outnumbers host cells greatly affects biology fitness. Ticks are hematophagous ectoparasites with a wide range. They vector number human animal pathogens also directly cause major economic losses livestock. Although several reports on tick midgut microbiota show diverse bacterial...
In addition to being vectors of pathogenic bacteria, ticks also harbor intracellular bacteria that associate with over generations, aka symbionts. The biological significance such bacterial symbiosis has been described in several tick species but its function Ixodes ricinus is not understood. We have previously shown I. are primarily inhabited by a single symbiont, Midichloria mitochondrii, an bacterium resides and reproduces mainly the mitochondria ovaries fully engorged females. To study...
The Propagation of Plasmodium spp. and Babesia/Theileria vertebrate blood stages relies on the mediated acquisition nutrients available within host's red cell (RBC). cellular processes uptake, trafficking metabolic processing host RBC proteins are thus crucial for intraerythrocytic development these parasites. In contrast to malarial Plasmodia, molecular mechanisms uptake major cytoplasmic protein hemoglobin remain widely unexplored in species. paper, we provide an updated comparison stage...
Abstract Background Plasmodium falciparum contains three genes encoding potential glutamate dehydrogenases. The protein encoded by gdha has previously been biochemically and structurally characterized. It was suggested that it is important for the supply of reducing equivalents during intra-erythrocytic development and, therefore, a suitable drug target. Methods gene NADP(H)-dependent GDHa disrupted reverse genetics in P. effect on antioxidant metabolic capacities resulting mutant parasites...
The vector competence of blood-feeding arthropods is influenced by the interaction between pathogens and immune system vector. Toll IMD (immune deficiency) signaling pathways play a key role in regulation innate immunity both Drosophila model insects. However, ticks (chelicerates), determination for pathogen acquisition transmission has not yet been fully explored. Here, we have mapped homologs insect European tick Ixodes ricinus , an important human animal diseases. We show that most genes...
Dermanyssus gallinae is a blood-feeding mite that parasitises wild birds and farmed poultry. Its remarkably swift processing of blood, together with the capacity to blood-feed during most developmental stages, makes this highly debilitating pest. To identify specific adaptations digestion haemoglobin-rich diet, we constructed compared transcriptomes from starved blood-fed stages parasite identified midgut-enriched transcripts. We noted midgut transcripts encoding cysteine proteases were...
Ticks are obligate hematophagous arthropods that transmit a wide range of pathogens to humans as well wild and domestic animals. They also harbor non-pathogenic microbiota, although our previous study has shown the diverse bacterial microbiome in midgut Ixodes ricinus is quantitatively poor lacks core. In artificial infections by capillary feeding ticks with two model bacteria (Gram-positive Micrococcus luteus Gram-negative Pantoea sp.), rapid clearance these microbes from was observed,...
During feeding on vertebrate hosts, ticks secrete saliva composed of a rich cocktail bioactive molecules modulating host immune responses. Although most the proteinaceous fraction tick is little immunogenicity, repeated mammalian hosts may lead to impairment feeding, preventing full engorgement. Here, we challenged rabbits with both Ixodes ricinus nymphs and adults observed formation specific antibodies against several salivary proteins. Repeated I. stages led gradual decrease in engorged...
Ticks are ectoparasites that feed on blood and have an impressive ability to consume process enormous amounts of host blood, allowing extremely long periods starvation between meals. The central role in the parasitic lifestyle ticks is played by midgut. This organ efficiently stores digests ingested serves as primary interface for transmission tick-borne pathogens. In this study, we used a label-free quantitative approach perform novel dynamic proteomic analysis midgut Ixodesricinus nymphs,...
An abstract is not available for this content so a preview has been provided. As you have access to content, full PDF via the 'Save PDF' action button.