A5085793596- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Plant-Microbe Interactions and Immunity
- Forensic Entomology and Diptera Studies
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Insect and Arachnid Ecology and Behavior
- Insect Utilization and Effects
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Plant Pathogenic Bacteria Studies
- Insect behavior and control techniques
- Leech Biology and Applications
- Soil Carbon and Nitrogen Dynamics
- Legume Nitrogen Fixing Symbiosis
- Mycorrhizal Fungi and Plant Interactions
- Microbial infections and disease research
- Plant Virus Research Studies
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Plant and Fungal Interactions Research
- Hymenoptera taxonomy and phylogeny
- Gut microbiota and health
- Ecology and biodiversity studies
- Composting and Vermicomposting Techniques
Kansas State University
2019-2024
Agriculture and Agri-Food Canada
2018-2024
Louisiana State University
2016
Swedish University of Agricultural Sciences
2012-2014
Culture-independent metagenomic methodologies have enabled detection and identification of microorganisms in various biological systems often revealed complex unknown microbiomes. In many organisms, the microbiome outnumbers host cells greatly affects biology fitness. Ticks are hematophagous ectoparasites with a wide range. They vector number human animal pathogens also directly cause major economic losses livestock. Although several reports on tick midgut microbiota show diverse bacterial...
Plastic pollution has emerged as one of the growing challenges due to their relatively low production cost, unique physiochemical properties, ease in access, and little or no alternatives its use are not only limited domestic, institutional, industrial purpose. This study is conducted examine micro plastics (MPs) contamination Narayani River - major drainage systems Nepal. The water samples from were taken five different locations first half June 2023 covering around seven kilometers (km)...
Abstract Background The lone star tick ( Amblyomma americanum ), an important vector of a wide range human and animal pathogens, is very common throughout the East Midwest USA. Ticks are known to carry non-pathogenic bacteria that may play role in their competence for pathogens. Several previous studies using high throughput sequencing (HTS) technologies reported commensal midgut as abundant diverse. In contrast, our preliminary survey field collected adult ticks, we found number...
The phylogenetic affiliations of organisms responsible for aerobic CO oxidation in hypersaline soils and sediments were assessed using media containing 3.8 M NaCl. CO-oxidizing strains the euryarchaeotes, Haloarcula, Halorubrum, Haloterrigena Natronorubrum, isolated from Bonneville Salt Flats (UT) Atacama Desert salterns (Chile). A halophilic euryarchaeote, Haloferax strain Mke2.3T, was Hawai'i Island saline cinders. Mke2.3T most closely related to larsenii JCM 13917T (97.0% 16S rRNA...
Ticks are obligate hematophagous arthropods transmitting a wide range of pathogens to humans and animals. They also harbor non-pathogenic microbiota, primarily in the ovaries midgut. In previous study on Ixodes ricinus, we used culture-independent approach showed diverse but quantitatively poor midgut bacterial microbiome. Our analysis revealed absence core microbiome, suggesting an environmental origin tick microbiota.A adult females collected by flagging from two localities Czech Republic...
House flies are important nuisance pests in a variety of confined livestock operations. More importantly, house known mechanical vectors numerous animal and human pathogens. Bovine respiratory disease (BRD) is an economically important, complex illness cattle associated with several bacteria viruses. The role the ecology transmission bacterial pathogens BRD not understood. Using culture-dependent culture-independent methods, we examined prevalence Mannheimia haemolytica, Pasteurella...
Serratia plymuthica are plant-associated, plant beneficial species belonging to the family Enterobacteriaceae. The members of genus ubiquitous in nature and their life style varies from endophytic free-living. S. AS9 is special interest for its ability inhibit fungal pathogens rapeseed promote growth. genome comprises a 5,442,880 bp long circular chromosome that consists 4,952 protein-coding genes, 87 tRNA genes 7 rRNA operons. This part project entitled "Genomics four growth promoting...
Abstract Background Biting midges ( Culicoides spp.) are important vectors of diverse microbes such as viruses, protozoa, and nematodes that cause diseases in wild domestic animals. However, little is known about the role microbial communities midge larval habitat utilization wild. In this study, we characterized (bacterial, protistan, fungal metazoan) soils from disturbed (bison cattle grazed) undisturbed (non-grazed) pond spring potential habitats. We evaluated influence grazing...
A plant-associated member of the family Enterobacteriaceae, Serratia plymuthica strain AS12 was isolated from rapeseed roots. It is scientific interest because it promotes plant growth and inhibits pathogens. The genome S. comprises a 5,443,009 bp long circular chromosome, which consists 4,952 protein-coding genes, 87 tRNA genes 7 rRNA operons. This sequenced within 2010 DOE-JGI Community Sequencing Program (CSP2010) as part project entitled "Genomics four promoting bacteria with...
Summary R hizobacteria with biocontrol ability exploit a range of mechanisms to compete successfully other microorganisms and ensure their growth survival in the rhizosphere, ultimately promoting plant growth. The rhizobacterium S erratia plymuthica AS 13 is able promote oilseed rape improve seedling presence fungal pathogen, hizoctonia solani AG 2‐1; however, our understanding underlying antagonism limited. To elucidate possible mechanisms, genome‐wide gene expression profiling . was...
Strain CS-1 T , a novel facultative anaerobic bacterium, was isolated from the larval gastrointestinal tract of biting midge, Culicoides sonorensis vector epizootic haemorrhagic disease virus and bluetongue virus. Cells were Gram-stain-positive, non-motile, non-spore-forming, pleomorphic rods. Optimal growth occurred at pH 7.5 37 °C. The G+C content genomic DNA 38.3 mol%, estimated by using HPLC. dominant cellular fatty acids C 14 : 0 (45.9 %) 16 (26.6 %). polar lipid profile comprised...
Abstract House flies (Musca domestica Linnaeus) are vectors of human and animal pathogens at livestock operations. Microbial communities in acquired from, correlate with, their local environment. However, variation among microbial carried by from farms different geographical areas is not well understood. We characterized bacterial female house collected beef dairy Oklahoma, Kansas, Nebraska using 16S rDNA amplicon sequencing PCR. Bacterial community composition was affected farm type...
Adult house flies frequent microbe-rich sites such as urban dumpsters and animal facilities, encounter ingest bacteria during feeding reproductive activities. Due to unique nutritional needs, male female demonstrate different interactions with substrates therefore dissemination potential. We investigated culturable aerobic coliform abundance in (n = 107) collected from (restaurant dumpsters) agricultural (dairy farm) sites. Whole-fly homogenate was aerobically cultured enumerated on...
Abstract Adult house flies feed and breed in a variety of microbe‐rich habitats serve as vectors for human animal pathogens. To better understand their role harbouring disseminating bacteria, we characterized the composition diversity bacterial communities gut female collected from three different Kansas: agricultural (dairy farm), urban (business area dumpsters) mixed located between residential agriculture areas). Bacterial community were influenced more by flies' habitat than sampling...
Abstract Problems with patchiness due to delay in emergence or poor crop establishment are not uncommon oilseed rape cultivation. Our aim was study the fungal communities associated field grown and explore their role early establishment. This carried out by characterising roots rhizosphere soils of both spring winter rape. The were analysed using culture-dependent culture-independent approaches. Sampling plots clear differences Phylogenetic comparison different environments revealed...
Abstract Background House fly larvae ( Musca domestica L.) require a live microbial community to successfully develop. Cattle manure is rich in organic matter and microorganisms, comprising suitable substrate for who feed on both the decomposing prokaryotic eukaryotic microbes therein. Microbial communities change as ages, when are present changes attributable larval grazing also occur. Here, we used high throughput sequencing of 16S 18S rRNA genes characterize dairy cattle evaluated those...
Abstract House flies, Musca domestica L., (Diptera: Muscidae) mechanically vector diverse disease-causing microorganisms while foraging for food in agricultural and urban habitats. Although flies are feeders, nutrient composition of is important both fly longevity reproduction, especially anautogenous females who require protein egg production. We investigated whether sex and/or mating status influenced their preference foods with varying macronutrient composition. Presumably mated or...