- Antimicrobial Resistance in Staphylococcus
- Genomics and Phylogenetic Studies
- Microbial bioremediation and biosurfactants
- Enzyme Production and Characterization
- Vibrio bacteria research studies
- Wastewater Treatment and Nitrogen Removal
- Viral Infections and Vectors
- Microbial Community Ecology and Physiology
- Insect and Pesticide Research
- Clostridium difficile and Clostridium perfringens research
- Salmonella and Campylobacter epidemiology
- Forensic Entomology and Diptera Studies
- Identification and Quantification in Food
- Insect Utilization and Effects
- Pharmaceutical and Antibiotic Environmental Impacts
- Probiotics and Fermented Foods
- Microbial Natural Products and Biosynthesis
- Enzyme Catalysis and Immobilization
- Pesticide and Herbicide Environmental Studies
- Insect symbiosis and bacterial influences
- Enterobacteriaceae and Cronobacter Research
- Escherichia coli research studies
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Streptococcal Infections and Treatments
Pittsburg State University
2017-2024
Jamia Millia Islamia
2023
Kansas State University
2011-2021
North Bengal Medical College and Hospital
2019
University of New Hampshire
2019
Technical University of Denmark
2015
University of Minnesota
2015
École Nationale Vétérinaire de Toulouse
2015
Université Claude Bernard Lyon 1
2015
Centre National de la Recherche Scientifique
2015
Abstract Background Extensive use of antibiotics as growth promoters in the livestock industry constitutes strong selection pressure for evolution and antibiotic resistant bacterial strains. Unfortunately, microbial ecology spread these bacteria agricultural, urban, suburban environments are poorly understood. Insects such house flies ( Musca domestica ) German cockroaches Blattella germanica can move freely between animal waste food may play a significant role dissemination within...
A bacterial strain Rhodococcus imtechensis RKJ300 (= MTCC 7085T = JCM 13270T) was isolated from pesticide-contaminated soil of Punjab by the enrichment technique on minimal medium containing 4-nitrophenol. Strain is capable utilizing 4-nitrophenol, 2-chloro-4-nitrophenol, and 2,4-dinitrophenol as sole sources carbon energy. The involved both oxidative reductive catabolic mechanisms for initial transformation these compounds. In case colorimetric analysis indicated that nitrite release...
The enterococcal community from feces of seven dogs treated with antibiotics for 2–9 days in the veterinary intensive care unit (ICU) was characterized. Both, culture-based approach and culture-independent 16S rDNA amplicon 454 pyrosequencing, revealed an abnormally large community: 1.4±0.8×108 CFU gram−1 48.9±11.5% total 16,228 sequences, respectively. diversity overall microbial very low which likely reflects a high selective antibiotic pressure. based on 210 isolates also as represented...
In the USA, small animal veterinary hospitals (SAVHs) commonly keep resident cats living permanently as pets within their facilities. Previously, multi-drug resistant (MDR) enterococci were found a contaminant of multiple surfaces such hospitals, and nosocomial infections are concern. The objectives this study to determine whether carry MDR compare feline isolates genotypically those obtained from SAVH in previous study. Enterococcal strains (n = 180) isolated feces six healthy different...
A Gram-positive, endospore-forming, alkalitolerant bacterial strain, designated MLB2 T , was isolated from soil Leh, India, and subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Bacillus . Growth observed at pH 7.0–11.0, but not 6.0. DNA G+C content 41.4 mol%. highest level of 16S rRNA gene sequence similarity oshimensis JCM 12663 (98.8 %). However, DNA–DNA...
Burkholderia sp. strain SJ98 is known for its chemotaxis towards nitroaromatic compounds (NACs) that are either utilized as sole sources of carbon and energy or co-metabolized in the presence alternative sources. Here we test this six chloro-nitroaromatic (CNACs), namely 2-chloro-4-nitrophenol (2C4NP), 2-chloro-3-nitrophenol (2C3NP), 4-chloro-2-nitrophenol (4C2NP), 2-chloro-4-nitrobenzoate (2C4NB), 4-chloro-2-nitrobenzoate (4C2NB) 5-chloro-2-nitrobenzoate (5C2NB), examine relationship to...
ABSTRACT Approximately 15% of foster kittens die before 8 weeks age, with most these demonstrating clinical signs or postmortem evidence enteritis. While a specific cause enteritis is not determined in cases, are often empirically administered probiotics that contain enterococci. The enterococci members the commensal intestinal microbiota but also can function as opportunistic pathogens. Given complicated role health and disease, it would be valuable to better understand what constitutes...
In June 2016, we continued surveillance for tick-borne viruses in eastern Kansas following upon a larger program initiated 2015 response to fatal human case of Bourbon virus (BRBV) (Family Orthomyxoviridae: Genus Thogotovirus). 4 d, collected 14,193 ticks representing four species from sites. Amblyomma americanum (L.) (Acari: Ixodidae) accounted nearly all (n = 14,116, 99.5%), and the only other identified were maculatum Koch Ixodidae), Dermacentor variabilis (Say) Ixodes scapularis Say...
A Gram-positive actinobacterium, strain RKJ300(T), capable of utilizing p-nitrophenol and 2,4-dinitrophenol, was isolated from a pesticide-contaminated site in India. The morphological chemotaxonomic properties the isolate were typical members genus Rhodococcus. DNA G+C content 72 mol%. Strain RKJ300(T) exhibited highest level sequence similarity with Rhodococcus wratislaviensis NCIMB 13082(T) (99.3 %), followed by opacus DSM 43205(T) (98.8 percolatus MBS1(T) (98.6 %) koreensis DNP505(T)...
The prevalence of gelatinase activity and biofilm formation among environmental enterococci was assessed. In total, 396 enterococcal isolates from swine cattle faeces house flies a farm were screened for activity. most prevalent phenotype on Todd-Hewitt agar with 1.5% skim milk the weak protease (WP) (72.2% isolates), followed by strong (SP) 18.7%, no (NP) (9.1%). majority WP represented Enterococcus hirae (56.9%), faecium (25.9%), casseliflavus (10.4%), gallinarum (5.2%) saccharolyticus...
This study's aims were to assess the prevalence of, and characterize, vancomycin-resistant enterococci (VRE) from rooks (Corvus frugilegus) wintering in Europe during 2010/2011. Faeces samples cultivated selectively for VRE characterized. Pulsed-field gel electrophoresis multilocus sequence typing (MLST) used examine epidemiologic relationships of vanA-containing VRE. The vanA-carrying tested vitro mobility vancomycin resistance traits. found 62 (6%) 1073 rook samples. Enterococcal species...
Abstract Aerobic microbial degradation of p -nitrophenol (PNP) has been classically shown to proceed via ‘Hydroquinone (HQ) pathway’ in Gram-negative bacteria, whereas Gram-positive PNP degraders it ‘Benzenetriol (BT) pathway’. These pathways are characterized by the ring cleavage HQ and BT as terminal aromatic intermediates respectively. Earlier reports on have indicated these be mutually exclusive. We report involvement both ‘HQ’ ‘BT’ Burkholderia sp. strain SJ98. Genetic characterization...
To determine the prevalence of bacterial contamination on 4 surfaces types standard equipment in small animal veterinary hospitals.Surveillance study.10 hospitals.Each hospital was visited 3 times at 4-month intervals; each visit, a cage door, stethoscope, rectal thermometer, and mouth gag were swabbed. Swab samples plated onto media for culture enterococci organisms family Enterobacteriaceae. Enterococci identified via species-specific PCR assay sodA gene sequencing; species...
Chlorophenols (CPs) are major environmental pollu-tants discharged from pulp and paper mills, tanneries,distilleries, dye paint manufacturing pharma-ceuticals industry (Crosby, 1981). The source ofCPs is as by-products when chlorine used forbleaching of for disinfections drinkingwater. Therefore, large amounts chlorinated aro-matic compounds, including CPs, have been dis-charged into the environment (Steinle et al., 1998).Among chlorophenols, pentachlorophenol (PCP) awide spectrum biocide...
A catabolite‐derepressed mutant strain of Aspergillus terreus NA‐170 was isolated by multi‐step mutagenesis. The produced an appreciable yield glucoamylase in the culture medium. enzyme purified and shown to be homogeneous. It hydrolysed successive glucose residues from non‐reducing end starch molecules. had optimum pH 5·0 stable over range 3·0–7·0. highly active a broad temperature range, 30–75°C, with optimal activity at 60°C. molecular weight 70 000 as determined Sephadex G‐200...
Strain CS-1 T , a novel facultative anaerobic bacterium, was isolated from the larval gastrointestinal tract of biting midge, Culicoides sonorensis vector epizootic haemorrhagic disease virus and bluetongue virus. Cells were Gram-stain-positive, non-motile, non-spore-forming, pleomorphic rods. Optimal growth occurred at pH 7.5 37 °C. The G+C content genomic DNA 38.3 mol%, estimated by using HPLC. dominant cellular fatty acids C 14 : 0 (45.9 %) 16 (26.6 %). polar lipid profile comprised...
Cattle manure is one of the primary larval developmental habitats house flies, Musca domestica (L.). serve as asymptomatic reservoirs Shiga toxin-producing Escherichia coli (STEC), and bacteria are released into environment in cattle feces. The USDA-FSIS declared seven STEC serogroups (O157, O26, O45, O103, O145, O121, O111) adulterants beef products. In addition, serogroup O104 was a culprit large outbreak Germany 2011. Our study aimed to assess prevalence non-O157 (O26, O111, O104) adult...
The states of Kansas and Oklahoma, in the central Great Plains, lie at western periphery geographic distributions several tick species. As focus most research on ticks tick-borne diseases has been Lyme disease which commonly occurs areas to north east, this region have seen little attention. Here, we report phenology activity patterns shown by species observed 10 sites across two explore factors associated with abundance all life specific individuals dominant Ticks were collected 2020–2022...
Two Gram-positive, catalase-negative bacterial strains were isolated from the cloaca of common kingfishers (Alcedo atthis). Repetitive sequence-based PCR fingerprinting using (GTG)5 primer grouped these isolates into a single cluster separated all known enterococcal species. The two revealed identical 16S rRNA gene sequences placing them within genus Enterococcus with aquimarinus LMG 16607(T) as closest relative (97.14 % similarity). Further taxonomic investigation sequencing genes for...