A5068535680- Vector-borne infectious diseases
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Vector-Borne Animal Diseases
- Trypanosoma species research and implications
- Neurobiology and Insect Physiology Research
- Study of Mite Species
- Toxin Mechanisms and Immunotoxins
- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Studies on Chitinases and Chitosanases
- Peptidase Inhibition and Analysis
- Viral Infections and Vectors
- Insect-Plant Interactions and Control
- Insect and Arachnid Ecology and Behavior
- Evolution and Genetic Dynamics
- Invertebrate Immune Response Mechanisms
Czech Academy of Sciences, Biology Centre
2010-2024
Institute of Parasitology
2010-2024
Czech Academy of Sciences
2022-2024
University of South Bohemia in České Budějovice
2010-2011
Abstract Background Ticks are vectors of a wide variety pathogens causing severe diseases in humans and domestic animals. Intestinal digestion the host blood is an essential process tick physiology also limiting factor for pathogen transmission since gut represents primary site infection proliferation. Using model Ixodes ricinus , European Lyme disease vector, we have previously demonstrated by genetic biochemical analyses that degraded network acidic peptidases aspartic cysteine classes....
Culture-independent metagenomic methodologies have enabled detection and identification of microorganisms in various biological systems often revealed complex unknown microbiomes. In many organisms, the microbiome outnumbers host cells greatly affects biology fitness. Ticks are hematophagous ectoparasites with a wide range. They vector number human animal pathogens also directly cause major economic losses livestock. Although several reports on tick midgut microbiota show diverse bacterial...
In addition to being vectors of pathogenic bacteria, ticks also harbor intracellular bacteria that associate with over generations, aka symbionts. The biological significance such bacterial symbiosis has been described in several tick species but its function Ixodes ricinus is not understood. We have previously shown I. are primarily inhabited by a single symbiont, Midichloria mitochondrii, an bacterium resides and reproduces mainly the mitochondria ovaries fully engorged females. To study...
Ticks are obligate hematophagous arthropods transmitting a wide range of pathogens to humans and animals. They also harbor non-pathogenic microbiota, primarily in the ovaries midgut. In previous study on Ixodes ricinus, we used culture-independent approach showed diverse but quantitatively poor midgut bacterial microbiome. Our analysis revealed absence core microbiome, suggesting an environmental origin tick microbiota.A adult females collected by flagging from two localities Czech Republic...
Ticks are obligate hematophagous arthropods that transmit a wide range of pathogens to humans as well wild and domestic animals. They also harbor non-pathogenic microbiota, although our previous study has shown the diverse bacterial microbiome in midgut Ixodes ricinus is quantitatively poor lacks core. In artificial infections by capillary feeding ticks with two model bacteria (Gram-positive Micrococcus luteus Gram-negative Pantoea sp.), rapid clearance these microbes from was observed,...
Ticks are ectoparasites that feed on blood and have an impressive ability to consume process enormous amounts of host blood, allowing extremely long periods starvation between meals. The central role in the parasitic lifestyle ticks is played by midgut. This organ efficiently stores digests ingested serves as primary interface for transmission tick-borne pathogens. In this study, we used a label-free quantitative approach perform novel dynamic proteomic analysis midgut Ixodesricinus nymphs,...
Ticks, notorious blood-feeders and disease-vectors, have lost a part of their genetic complement encoding haem biosynthetic enzymes are, therefore, dependent on the acquisition distribution host haem. Solute carrier protein SLC48A1, aka haem-responsive gene 1 (HRG1), has been implicated in transport, regulating availability intracellular HRG1 transporter identified both free-living parasitic organisms ranging from unicellular kinetoplastids, nematodes, up to vertebrates. However, an...
It has been demonstrated that impairing protein synthesis using drugs targeted against tRNA amino acid synthetases presents a promising strategy for the treatment of wide variety parasitic diseases, including malaria and toxoplasmosis. This is first study evaluating as potential drug targets in ticks. RNAi knock-down all tested had strong deleterious phenotype on Ixodes ricinus feeding. Our data indicate represent attractive, anti-tick warranting design selective inhibitors. Further, we...
The control of ticks through vaccination offers a sustainable alternative to the use chemicals that cause contamination and selection resistant tick strains. However, only limited number anti-tick vaccines have reached commercial realization. In this sense, an antigen effective against different species is desirable target for developing such vaccines. A peptide derived from P0 protein (pP0) conjugated carrier has been demonstrated be Rhipicephalus microplus, sanguineus, Amblyomma mixtum...