A5002521144- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Education, Psychology, and Social Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Light effects on plants
- Plant Disease Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Resistance
- Advanced Proteomics Techniques and Applications
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Glycosylation and Glycoproteins Research
- Plant Genetic and Mutation Studies
- Seed Germination and Physiology
- Potato Plant Research
- Plant and Fungal Interactions Research
- Botany and Plant Ecology Studies
- Microbial Metabolites in Food Biotechnology
- Pleistocene-Era Hominins and Archaeology
- Phytase and its Applications
- Plant Growth Enhancement Techniques
- Plant Micronutrient Interactions and Effects
Mendel University in Brno
2015-2025
Central European Institute of Technology
2013-2024
Masaryk University
2024
Central European Institute of Technology – Masaryk University
2024
Czech Academy of Sciences, Institute of Archaeology, Prague
2022-2023
Charles University
1979-2021
Czech Academy of Sciences, Institute of Biophysics
2010-2018
State Key Laboratory of Building Safety and Built Environment
2012
Institute of Landscape Ecology of the Slovak Academy of Sciences
1990
Institute of Criminology
1987
Our phenotyping and hormonal study has been aimed to characterize the role of cytokinins (CK) in drought recovery responses Arabidopsis thaliana. CK down-regulation was achieved by overexpression gene for deactivating enzyme cytokinin oxidase/dehydrogenase (CKX): constitutive (35S:CKX) or at stress onset using a dexamethasone-inducible pOp/LhGR promoter (DEX:CKX). The 35S:CKX plants exhibited slow ontogenesis higher expression levels stress-associated genes, e.g. AtP5CS1, already control...
Significance DELLA proteins are plant-specific transcriptional regulators that act as signaling hubs at the interface between environment and networks control growth. The growth-promoting hormone gibberellin destabilizes DELLAs. Here we describe an alternative pathway to destabilize these proteins. We show DELLAs substrate of COP1, E3 ubiquitin ligase increases its activity promote growth in response shade or warmth. Our results not changes levels, mediates rapid destabilization environmental cues.
The phytohormone cytokinin has been shown to affect many aspects of plant development ranging from the regulation shoot apical meristem leaf senescence. However, some studies have reported contradictory effects on physiology. Therefore treatments cause both chlorosis and increased greening lead decrease or increase in cell size. To elucidate this multifaceted role development, we employed a system temporal controls over pool investigated consequences modulated levels third Arabidopsis. We...
As sessile organisms, plants must sense environmental conditions and adjust their growth development processes accordingly, through adaptive responses regulated by various internal factors, including hormones. A key factor is temperature, but temperature-sensing mechanisms are not fully understood despite intense research. We investigated proteomic to temperature shocks (15 min cold or heat treatments) with without exogenous applications of cytokinin in Arabidopsis. Image mass spectrometric...
Rice is a plant species sensitive to cold stress, which renders seriously its cultivation. Responses stress (5°C, 24 hours) in whole plants, or targeted rice leaves roots were followed at the hormonome, transcriptome, proteome, and sugar levels, find organ-specific responses processes affected by acclimation. Targeted stresses caused proteomic changes mainly unexposed organs. An increase abscisic acid (ABA) was accompanied decrease jasmonic (JA) (in non-stressed leaves) vice versa (JA...
Cytokinins are plant hormones involved in regulation of diverse developmental and physiological processes plants whose molecular mechanisms action being intensely researched. However, most rapid responses to cytokinin signals at the proteomic phosphoproteomic levels unknown. Early were investigated through proteome-wide expression profiling based on image mass spectrometric analysis two-dimensionally separated proteins phosphoproteins. The effects 15 min treatments 7-day-old Arabidopsis...
In plants, numerous developmental processes are controlled by cytokinin (CK) levels and their ratios to of other hormones. While molecular mechanisms underlying the regulatory roles CKs have been intensely researched, proteomic metabolomic responses CK deficiency unknown. Transgenic Arabidopsis seedlings carrying inducible barley oxidase/dehydrogenase (CaMV35S>GR>HvCKX2) agrobacterial isopentenyl transferase (CaMV35S>GR>ipt) constructs were profiled elucidate proteome- metabolome-wide down-...
Cytokinins are positive regulators of shoot development. However, it has previously been demonstrated that efficient activation the cytokinin biosynthesis gene ipt can cause necrotic lesions and wilting in tobacco leaves. Some plant pathogens reportedly use their ability to produce cytokinins disease In response pathogen attacks, plants trigger a hypersensitive rapidly kills cells near infection site, depriving nutrients preventing its spread. this study, diverse set processes link lesion...
Cytokinins (CKs) are phytohormones regulating plant growth and development as well response to the environment. In order evaluate their function in heat stress (HS) responses, effect of CK elevation was determined during three types HS – targeted shoots, roots applied whole plant. The early (30min) longer term (3h) responses were followed at hormonal, transcriptomic proteomic levels Arabidopsis transformants with dexamethasone-inducible expression biosynthetic gene isopentenyltransferase...
To elucidate the effect of light intensity on cold response (5°C; 7 days) in Arabidopsis thaliana , we compared following parameters under standard (150 μmol m –2 s –1 ), low (20 and dark conditions: membrane damage, photosynthetic parameters, cytokinin oxidase/dehydrogenase (CKX) activity, phytohormone levels, transcription selected stress- hormone-related genes proteome. The impact cytokinins (CKs), hormones directly interacting with signaling pathway, responses was evaluated using...
Cytokinin is an indispensable phytohormone responsible for physiological processes ranging from root development to leaf senescence. The term "cytokinin" refers several dozen adenine-derived compounds occurring naturally in plants. Cytokinins (CKs) can be divided into various classes and forms; base forms are generally considered active while highly abundant cytokinin-N-glucosides (CKNGs), composed of a CK irreversibly conjugated glucose molecule, inactive. However, results early studies...
We introduce a new allocation rule, the uniform-dividend value (UD-value), for cooperative games whose characteristic function is incomplete. The UD-value assigns payoffs by distributing total surplus of each family indistinguishable coalitions uniformly among them. Our primary focus on set systems that are intersection-closed, which we show uniquely determined and can be interpreted as expected Shapley over all positive (i.e., nonnegative-surplus) extensions incomplete game. compare to two...
Understanding how plants distinguish between commensal and pathogenic microorganisms is one of the major challenges in plant microbe interaction research. We previously identified a gene encoding CYP71A27 connected to camalexin, which necessary for growth promoting (PGP) activity number bacterial strains. To dissect its function, we compared multilevel responses roots wild type Arabidopsis cyp71A27 mutant two strains, PGP Pseudomonas fluorescens CH267 pathogen Burkholderia glumeae PG1. show...
Plant reactions to stress vary with development stage and fitness. This study assessed the relationship between light chilling in Arabidopsis acclimation. By analysing transcriptome proteome responses of expanding leaves subjected varying intensity cold, 2251 2064 early response genes proteins were identified, respectively. Many these represent as a yet unknown part illustrating development-dependent duality plant adaptations. While standard promoted photosynthetic upregulation, plastid...
In nature, root systems of most terrestrial plants are protected from light exposure by growing in a dark soil environment. Hence, vitro cultivation transparent Petri dishes leads to physiological perturbations, but the mechanisms underlying root-mediated perception and responses have not been fully elucidated. Thus, we compared Arabidopsis thaliana seedling development darkened at low intensity (20 µmol m−2 s−1), allowing us follow (inter alia) hypocotyl elongation, which is an excellent...
Cytokinin is a phytohormone involved in the regulation of diverse developmental and physiological processes plants. Its potential biotechnology for development higher-yield more resilient plants has been recognized, yet molecular mechanisms behind its action are far from understood. In this report, roots barley seedlings were explored as new source to reveal unknown cytokinin-responsive proteins crop improvement. Here we found significant differences reproducibly observed 178 proteins, which...