A5070093006- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Heat shock proteins research
- Plant Gene Expression Analysis
- Endoplasmic Reticulum Stress and Disease
- RNA Research and Splicing
- Plant Stress Responses and Tolerance
- Light effects on plants
- RNA modifications and cancer
- Plant responses to water stress
- Mitochondrial Function and Pathology
- Leaf Properties and Growth Measurement
- Genomics and Chromatin Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Cell Image Analysis Techniques
- MicroRNA in disease regulation
- Glycosylation and Glycoproteins Research
- Plant Growth Enhancement Techniques
- Plant and animal studies
- Transgenic Plants and Applications
- Circadian rhythm and melatonin
University of Lausanne
2023-2024
Instituto de Biología Molecular y Celular de Plantas
2016-2024
Consejo Superior de Investigaciones Científicas
2017-2024
Universitat Politècnica de València
2016-2024
ETH Zurich
2021-2023
École Polytechnique Fédérale de Lausanne
2022
Biomar Microbial Technologies (Spain)
2016
Universidad de Salamanca
2016
Significance DELLA proteins are plant-specific transcriptional regulators that act as signaling hubs at the interface between environment and networks control growth. The growth-promoting hormone gibberellin destabilizes DELLAs. Here we describe an alternative pathway to destabilize these proteins. We show DELLAs substrate of COP1, E3 ubiquitin ligase increases its activity promote growth in response shade or warmth. Our results not changes levels, mediates rapid destabilization environmental cues.
With growing populations and pressing environmental problems, future economies will be increasingly plant-based. Now is the time to reimagine plant science as a critical component of fundamental science, agriculture, stewardship, energy, technology healthcare. This effort requires conceptual technological framework identify map all cell types, comprehensively annotate localization organization molecules at cellular tissue levels. framework, called Plant Cell Atlas (PCA), for understanding...
DELLA proteins are negative regulators of the gibberellin response pathway in angiosperms, acting as central hubs that interact with hundreds transcription factors (TFs) and to modulate their activities. While mechanism TF sequestration by DELLAs prevent DNA binding downstream targets has been extensively documented, allows them act coactivators remains be understood. Here, we demonstrate directly recruit Mediator complex specific loci Arabidopsis, facilitating transcription. This...
SUMMARY Ovule development is a key process for plant reproduction, helping to ensure correct seed production. Several molecular factors and hormones such as gibberellins are involved in ovule initiation development. Gibberellins control by the destabilization of DELLA proteins, whereas activity has been shown act positive factor primordia emergence. But mechanism which acts remained unknown. In this study we report that proteins participate formation protein complex with CUC2 transcription...
ABSTRACT The thickening of plant organs is supported by secondary growth, a process which new vascular tissues (xylem and phloem) are produced. Xylem composed several cell types, including xylary fibers, parenchyma vessel elements. In Arabidopsis, it has been shown that fibers promoted the class-I KNOX gene KNAT1 hormones gibberellins, repressed small set receptor-like kinases; however, we lack mechanistic framework to integrate their relative contributions. Here, show DELLAs, negative...
Although originally identified as the components of complex aiding cytosolic chaperonin CCT in folding actins and tubulins cytosol, prefoldins (PFDs) are emerging novel regulators influencing gene expression nucleus. Work conducted mainly yeast animals showed that PFDs act transcriptional participate nuclear proteostasis. To investigate new functions PFDs, we performed a co-expression analysis Arabidopsis thaliana. Results revealed between PFD Sm-like (LSM) genes, which encode LSM2-8...
Brassinosteroid signaling is essential for plant growth as exemplified by the dwarf phenotype of loss-of-function mutants in BRASSINOSTEROID INSENSITIVE 1 ( BRI1 ), a ubiquitously expressed Arabidopsis brassinosteroid receptor gene. Complementation brassinosteroid-blind expression with various tissue-specific promoters implied that local may instruct non–cell autonomously. Here, we performed such rescues panel variants and promoters, combination transgene knockouts. Our experiments...
Significance Secondary growth (thickening of stems and roots) provides the mechanical support that plants need to grow sustain their structures, results in wood formation. Although accounts for largest proportion terrestrial biomass, identification through classical genetics key elements control secondary is hampered by challenge phenotyping traits associated with inner tissues. We have taken advantage natural genetic variation identify SOBIR1/EVR, a gene previously known its involvement...
The evolutionarily conserved POLYMERASE-ASSOCIATED FACTOR1 complex (Paf1C) participates in transcription, and research animals fungi suggests that it facilitates RNA POLYMERASE II (RNAPII) progression through chromatin. We examined the genomic distribution of EARLY FLOWERING7 (ELF7) VERNALIZATION INDEPENDENCE3 subunits Paf1C Arabidopsis (Arabidopsis thaliana). occupancy both was confined to thousands gene bodies positively associated with RNAPII level expression, supporting a role as...
The presence of antibiotics in soils could be due to natural production by soil microorganisms or the effect anthropogenic activities. However, impact these compounds on plant physiology has not been thoroughly investigated. To evaluate β-lactam (carbenicillin and penicillin) growth development Arabidopsis thaliana roots, plants were grown different amounts we found a reduction root size, an increase size hairs as well abnormal position closer tip roots. Those phenomena dependent...
Abstract Plants undergo transcriptome reprograming to adapt daily and seasonal fluctuations in light temperature conditions. While most efforts have focused on the role of master transcription factors, importance splicing factors modulating these processes is now emerging. Efficient pre-mRNA depends proper spliceosome assembly, which plants animals requires methylosome complex. Ion Chloride nucleotide-sensitive protein (PICLN) part complex both humans Arabidopsis (Arabidopsis thaliana), we...
The prefoldin complex (PFDc) was identified in humans as a co-chaperone of the cytosolic chaperonin T-COMPLEX PROTEIN RING COMPLEX (TRiC)/CHAPERONIN CONTAINING TCP-1 (CCT). PFDc is conserved eukaryotes and composed subunits PFD1-6, PFDc-TRiC/CCT folds actin tubulins. PFDs also participate wide range cellular processes, both cytoplasm nucleus, their malfunction causes developmental alterations disease animals altered growth environmental responses yeast plants. Genetic analyses indicate that...
Efficient root-to-shoot delivery of water and nutrients in plants relies on the correct differentiation xylem cells into hollow elements. While auxin is integral to formation cells, it remains poorly characterized how each subcellular pool this hormone regulates process. Combining genetic cell biological approaches, we investigated bipartite activity nucleoplasmic vs plasma membrane-associated phosphatidylinositol 4-phosphate kinases PIP5K1 its homolog PIP5K2 Arabidopsis thaliana roots...
High temperature is a general stress factor that causes decrease in crop yield. It has been shown auxin application reduces the male sterility caused by exposure to higher temperatures. However, widespread of hormone with vast effects on plant physiology may be discouraged many cases. Therefore, generation new varieties locally enhance reproductive organs represent an alternative strategy. We have explored possibility increasing indole-3-acetic acid (IAA) ovaries reducing IAA...
ABSTRACT Over time, plants have evolved flexible self-organizing patterning mechanisms to adapt tissue functionality for continuous organ growth. An example of this process is the multicellular organization cells into a vascular network in foliar organs. important, yet poorly understood component secondary vein branching, mechanism employed extend tissues throughout cotyledon surface. Here, we uncover two distinct branching during embryogenesis by analyzing discontinuous double mutant...
Abstract Communication between the gametophytes is vital for angiosperm fertilisation. Multiple CrRLK1L-type receptor kinases prevent premature pollen tube burst, while another CrRLK1L protein, FERONIA (FER), required burst in female gametophyte. We report here identification of two additional homologues, HERCULES RECEPTOR KINASE 1 (HERK1) and ANJEA (ANJ), which act redundantly to promote at synergid cells. HERK1 ANJ localise filiform apparatus cells unfertilised ovules, herk1 anj mutants a...
Abstract DELLA proteins are negative regulators of the gibberellin response pathway in angiosperms, acting as central hubs that interact with hundreds transcription factors and to modulate their activities. While mechanism factor sequestration by DELLAs prevent DNA binding downstream targets has been extensively documented, allows them act co-activators remains be understood. Here, we demonstrate directly recruit Mediator complex specific loci Arabidopsis, facilitating transcription. This...
Abstract When exposed to stress, plants slow down their growth while activating defensive mechanisms. This behaviour has been proposed help reallocate resources and meet the energy demands required for survival. In this study, we show instead that can grow under limited water availability without compromising tolerance stress. However, cells continue divide stress accumulate DNA damage, which frequently leads cell death. Given lesions are observed in apical stem give rise all plant organs,...