A5065427790- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Microtubule and mitosis dynamics
- Plant tissue culture and regeneration
- Light effects on plants
- Plant responses to water stress
- Tree Root and Stability Studies
- Cellular transport and secretion
- Plant Water Relations and Carbon Dynamics
- Plant-Microbe Interactions and Immunity
- Genetics, Aging, and Longevity in Model Organisms
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Plant and Biological Electrophysiology Studies
- Neurobiology and Insect Physiology Research
- Biotin and Related Studies
- Interdisciplinary Research and Collaboration
- Molecular Junctions and Nanostructures
- Plant Physiology and Cultivation Studies
Stanford University
2016-2025
Howard Hughes Medical Institute
2016-2025
Stanford Medicine
2024
History of Science Society
2022
Child Trends
2016-2019
Ithaca College
2012-2016
The Geneva Association
2016
Berkeley College
2016
Advisory Board Company (United States)
2016
Paul Pascal Research Center
2016
Stomata are epidermal structures that modulate gas exchange between a plant and its environment. During development, stomata specified positioned nonrandomly by the integration of asymmetric cell divisions intercellular signaling. The Arabidopsis mitogen-activated protein kinase gene, YODA, acts as part molecular switch controlling identities in epidermis. Null mutations YODA lead to excess stomata, whereas constitutive activation eliminated stomata. Transcriptome analysis seedlings with...
Stomata are innovations of land plants that allow regulated gas exchange. Stomatal precursor cells produced by asymmetric cell division, and once formed, signal their neighbors to inhibit the formation stomatal precursors in direct contact. We report a gene Arabidopsis thaliana , EPIDERMAL PATTERNING FACTOR 1 ( EPF1 ) encodes small secretory peptide expressed controls patterning through regulation division. activity is dependent on TOO MANY MOUTHS receptor-like protein ERECTA family receptor...
Coordination between cell proliferation and differentiation is essential to create organized functional tissues. Arabidopsis thaliana stomata are created through a stereotyped series of symmetric asymmetric divisions whose frequency orientation informed by cell-cell interactions. Receptor-like proteins mitogen-activated protein kinase were previously identified as negative regulators stomatal development; here, we present the characterization bona fide positive regulator. FAMA putative basic...
Stomata, epidermal structures that modulate gas exchange between plants and the atmosphere, play critical roles in primary productivity global climate. Positively acting transcription factors negatively mitogen-activated protein kinase (MAPK) signaling control stomatal development Arabidopsis ; however, it is not known how opposing activities of these regulators are integrated. We found a unique domain basic helix-loop-helix (bHLH) initiating factor, SPEECHLESS, renders MAPK phosphorylation...
Plants optimize carbon assimilation while limiting water loss by adjusting stomatal aperture. In grasses, a developmental innovation-the addition of subsidiary cells (SCs) flanking two dumbbell-shaped guard (GCs)-is linked to improved physiology. Here, we identify transcription factor necessary and sufficient for SC formation in the wheat relative Brachypodium distachyon. Unexpectedly, is an ortholog regulator AtMUTE, which defines GC precursor fate Arabidopsis The novel role BdMUTE...
Defining specific protein interactions and spatially or temporally restricted local proteomes improves our understanding of all cellular processes, but obtaining such data is challenging, especially for rare proteins, cell types, events. Proximity labeling enables discovery neighborhoods defining functional complexes and/or organellar compositions. Recent technological improvements, namely two highly active biotin ligase variants (TurboID miniTurbo), allowed us to address challenging...
Lineage-specific stem cells are critical for the production and maintenance of specific cell types tissues in multicellular organisms. In Arabidopsis, initiation proliferation stomatal lineage is controlled by basic helix-loop-helix transcription factor SPEECHLESS (SPCH). SPCH-driven asymmetric self-renewing divisions allow flexibility overall organ growth. How SPCH directs behaviors, however, unclear. Here, we improved chromatin immunoprecipitation (ChIP) assay profiled genome-wide targets...
Stomata are adjustable pores in the plant epidermis that regulate gas exchange between and atmosphere; they present on aerial portions of most higher plants. Genetic pathways controlling stomatal development distribution have been described some detail for one dicot species, Arabidopsis, which three paralogous bHLH transcription factors, FAMA, MUTE SPCH, control discrete sequential stages development. Orthologs SPCH other flowering This observation is particular interest when considering...
Abstract Mitogen-activated protein kinase (MAPK) signaling networks regulate numerous eukaryotic biological processes. In Arabidopsis thaliana, that contain MAPK kinases MKK4/5 and MAPKs MPK3/6 function in abiotic biotic stress responses embryonic stomatal development. However, how single modules direct specific output signals without cross-activating additional downstream processes is largely unknown. Studying relationships between components outcomes difficult because broad experimental...
• Stomatal conductance (g(s)) is constrained by the size and number of stomata on plant epidermis, potential maximum rate g(s) can be calculated based these stomatal traits (Anatomical g(smax)). However, relationship between Anatomical g(smax) operational under atmospheric conditions remains undefined. Leaf-level gas-exchange measurements were performed for six Arabidopsis thaliana genotypes that have different profiles resulting from mutations or transgene activity in development. We found...
Summary Genetic and cell biological mechanisms that regulate stomatal development are necessary to generate an appropriate number of stomata enforce a minimum spacing one epidermal between stomata. The ability manipulate these processes in model plant system allows us investigate the physiological importance patterning changes density, therein testing underlying theories about biology. Twelve A rabidopsis thaliana genotypes have varied characteristics as result mutations or transgenes were...
Complex organisms consist of a multitude cell types arranged in precise spatial relation to each other. Arabidopsis roots generally exhibit radial tissue organization; however, within layer, cells are not identical. Specific vascular diametrically opposed longitudinal files that maximize the distance between them and create bilaterally symmetric (diarch) root. Mutations LONESOME HIGHWAY (LHW) gene eliminate bilateral symmetry reduce number center root, resulting with only single xylem phloem...
In plants, changes in local auxin concentrations can trigger a range of developmental processes as distinct tissues respond differently to the same stimulus. However, little is known about how interpreted by individual cell types. We performed transcriptomic analysis responses within four Arabidopsis thaliana root and demonstrate that different types show competence for discrete responses. The majority auxin‐responsive genes displayed spatial bias their induction or repression. novel data...
Stomata, epidermal valves facilitating plant-atmosphere gas exchange, represent a powerful model for understanding cell fate and pattern in plants. Core basic helix-loop-helix (bHLH) transcription factors regulating stomatal development were identified Arabidopsis, but this dicot's developmental morphology only one of many possibilities nature. Here, using unbiased forward genetic screens, followed by analysis reporters engineered mutants, we show that initiation the grass Brachypodium...