A5066722463- Plant Reproductive Biology
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Genetic Mapping and Diversity in Plants and Animals
- Plant and Fungal Interactions Research
- Cancer-related molecular mechanisms research
- Plant Genetic and Mutation Studies
- Genomics and Phylogenetic Studies
- Legume Nitrogen Fixing Symbiosis
- Wheat and Barley Genetics and Pathology
- Seed Germination and Physiology
- Plant Stress Responses and Tolerance
- Plant and animal studies
- Bioenergy crop production and management
- Photosynthetic Processes and Mechanisms
- Plant Taxonomy and Phylogenetics
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Crop Yield and Soil Fertility
- Plant Pathogens and Fungal Diseases
- Insect and Arachnid Ecology and Behavior
- Cell Image Analysis Techniques
Carnegie Institution for Science
2016-2025
Carnegie Department of Plant Biology
2013-2023
University of Wisconsin–Madison
1997-2010
University of Pennsylvania
1994
Abstract Background Long non-coding RNAs (lncRNAs) are transcripts that 200 bp or longer, do not encode proteins, and potentially play important roles in eukaryotic gene regulation. However, the number, characteristics expression inheritance pattern of lncRNAs maize still largely unknown. Results By exploiting available public EST databases, whole genome sequence annotation RNA-seq datasets from 30 different experiments, we identified 20,163 putative lncRNAs. Of these lncRNAs, more than 90%...
Abstract Angiosperm embryo sac development begins with a phase of free nuclear division followed by cellularization and differentiation cell types. The indeterminate gametophyte1 (ig1) gene maize (Zea mays) restricts the proliferative female gametophyte development. ig1 mutant gametophytes have prolonged divisions leading to variety abnormalities, including extra egg cells, polar nuclei, synergids. Positional cloning was performed based on genome sequence orthologous region in rice. encodes...
Abstract The Arabidopsis thaliana REVOLUTA (REV) protein is a member of the class III homeodomain-leucine zipper (HD-ZIPIII) proteins. REV potent regulator leaf polarity and vascular development. Here, we report identification gene family that encodes small leucine zipper–containing proteins (LITTLE ZIPPER [ZPR] proteins) where similar to found in REV, PHABULOSA, PHAVOLUTA transcript levels ZPR genes increase response activation steroid-inducible protein. We show interact with vitro ZPR3...
With growing populations and pressing environmental problems, future economies will be increasingly plant-based. Now is the time to reimagine plant science as a critical component of fundamental science, agriculture, stewardship, energy, technology healthcare. This effort requires conceptual technological framework identify map all cell types, comprehensively annotate localization organization molecules at cellular tissue levels. framework, called Plant Cell Atlas (PCA), for understanding...
Abstract Background Plant gametophytes play central roles in sexual reproduction. A hallmark of the plant life cycle is that gene expression required haploid gametophytes. Consequently, many mutant phenotypes are expressed this phase. Results We perform a quantitative RNA-seq analysis embryo sacs, comparator ovules with sacs removed, mature pollen, and seedlings to assist identification gametophyte functions maize. Expression levels were determined for annotated genes both gametophytes,...
In flowering plants, gene expression in the haploid male gametophyte (pollen) is essential for sperm delivery and double fertilization. Pollen also undergoes dynamic epigenetic regulation of from transposable elements (TEs), but how this process interacts with not clearly understood. To explore relationships among these processes, we quantified transcript levels four reproductive stages maize (tassel primordia, microspores, mature pollen, cells) via RNA-seq. We found that, contrast...
ABSTRACT Vegetative development in maize is divided into a juvenile phase and an adult that differ the expression of large number morphological, anatomical, biochemical traits. Recessive mutations Glossy15 cause premature switch some these phase-specific Mutant plants cease producing traits (e.g. epicuticular wax) begin to produce epidermal hairs) significantly earlier than their wild-type siblings. In glossy15-1 this generally occurs at leaf 2 or 3 rather normal position 6 7. An analysis...
Heteroblastic features of leaf anatomy in maize were identified by conducting a quantitative analysis anatomy. variation cuticle thickness and epidermal cell shape paralleled changes previously defined juvenile- adult-specific traits. The other traits examined this study (thickness the blade, bundle sheath size, vascular area, interveinal distance, mesophyll area : ratio) varied more complex fashion. To determine which these are regulated genes involved shoot maturation, we effect Teopod2...
Major genes govern the fertilization of teosinte ovules by maize pollen. A pollen–pistil compatibility system different from previously described systems, Ga1-s and Tcb1-s, was identified among lines introgressed with chromosome segments 2 populations. The pistil barrier is dominant, pollen competence determined genotype individual grain. major gene governing this incompatibility behaves as a strong allele ga2, locus genetic stocks on basis transmission ratio distortion. Additionally,...
Recent progress has been made in the genetic dissection of angiosperm shoot apical meristem (SAM) structure and function. Genes required for proper SAM development have identified a variety species through isolation mutants. In addition, genes with expression patterns indicating they play role function molecularly. The processes formation, self-renewal, pattern formation within are examined an emphasis on contributions recent classical molecular experiments to our understanding this basic...
In angiosperms, double fertilization of an egg cell and a central with two sperm cells results in the formation seed containing diploid embryo triploid endosperm. The extent to which sac controls postfertilization events is unknown. novel gametophytic maternal-effect maize mutation, baseless1 (bsl1) affects development within sac, frequently by altering position polar nuclei. Despite this irregularity, as efficient wild type. spatial expression basal endosperm-specific transcripts altered...
Abstract A central problem in speciation is the origin and mechanisms of reproductive barriers that block gene flow between sympatric populations. Wind-pollinated plant species flower synchrony with one another rely on post-pollination interactions to maintain isolation. In some locations Mexico, populations domesticated maize annual teosinte grow intimate associate synchronously, but rarely produce hybrids. This trait typically conferred by a single haplotype, Teosinte crossing barrier1-s ....
Abstract Three cross-incompatibility loci each control a distinct reproductive barrier in both domesticated maize (Zea mays ssp. mays) and its wild teosinte relatives. These three loci, Teosinte crossing barrier1 (Tcb1), Gametophytic factor1 (Ga1), Ga2, play key role preventing hybridization between incompatible populations are proposed to maintain the subspecies. Each locus encodes silk-active matching pollen-active pectin methylesterase (PMEs). To investigate diversity molecular evolution...
The plant life cycle alternates between two genetically active generations: the diploid sporophyte and haploid gametophyte. In angiosperms gametophytes are sexually dimorphic consist of only a few cells. female gametophyte, or embryo sac, is comprised four cell types: synergids, an egg cell, central variable number antipodal some species cells indistinct fail to proliferate, so many aspects function development have been unclear. maize other grasses, proliferate produce highly distinct...
Abstract Double fertilization of the embryo sac by two sperm cells a pollen grain initiates seed development. Proper development depends not only on action genes from resulting and endosperm, but also maternal acting at stages. Mutations with both sporophytic effects gametophytic have been identified. A new effect mutation in maize, lethal1 (mel1), causes production defective mutant female gametophytes. It shows reduced transmission, suggesting requirement male gametophyte, has no paternal...
Flowering plants, like placental mammals, have an extensive maternal contribution toward progeny development. Plants are distinguished from animals by a genetically active haploid phase of growth and development between meiosis fertilization, called the gametophyte. plants further process double fertilization that produces sister progeny, endosperm embryo, seed. Because this, there is substantial gene expression in female gametophyte contributes to regulation A primary function provide...
Many higher eukaryotes have evolved strategies for the maternal control of growth and development their offspring. In plants this is achieved in part by postmeiotic gene activity controlling haploid female gametophyte. stunter1 (stt1) a novel, recessive, effect mutant maize that displays viable, miniature kernels. Maternal inheritance stt1 results seeds with reduced but otherwise normal endosperms embryos. The mutation transmission through male parents causes significant changes sizes both...
Parent-of-origin-effect loci have non-Mendelian inheritance in which phenotypes are determined by either the maternal or paternal allele alone. In angiosperms, parent-of-origin effects can be caused required for gametophyte development imprinted genes needed seed development. Few parent-of-origin-effect been identified maize (Zea mays) even though there a large number of known from transcriptomics. We screened rough endosperm (rgh) mutants using reciprocal crosses with inbred parents. Six...