A5015883206- Plant Molecular Biology Research
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Genetic Mapping and Diversity in Plants and Animals
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Plant Gene Expression Analysis
- Plant Surface Properties and Treatments
- Postharvest Quality and Shelf Life Management
- Cancer-related molecular mechanisms research
- Plant tissue culture and regeneration
- Christian Theology and Mission
- Plant Genetic and Mutation Studies
- Tree Root and Stability Studies
- Molecular Biology Techniques and Applications
- Genetic and phenotypic traits in livestock
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- Genomics and Chromatin Dynamics
- Genetics and Plant Breeding
- CRISPR and Genetic Engineering
- Augustinian Studies and Theology
- Marine and coastal plant biology
- Plant Stress Responses and Tolerance
- Plant and Fungal Interactions Research
Cornell University
2016-2025
Plant (United States)
2019
Brigham Young University
2018
Iowa State University
1993-2012
Truman State University
2008-2012
University of Georgia
1998-2012
Cold Spring Harbor Laboratory
2012
University of California, Davis
2009
Piedmont Athens Regional
2006
University of California, Berkeley
1997
Abstract Background Long non-coding RNAs (lncRNAs) are transcripts that 200 bp or longer, do not encode proteins, and potentially play important roles in eukaryotic gene regulation. However, the number, characteristics expression inheritance pattern of lncRNAs maize still largely unknown. Results By exploiting available public EST databases, whole genome sequence annotation RNA-seq datasets from 30 different experiments, we identified 20,163 putative lncRNAs. Of these lncRNAs, more than 90%...
Significance Plants possess the remarkable ability to grow and produce new organs throughout their lifespan, owing activities of persistent populations pluripotent stem cells within meristematic tips. Here we isolated individual from microscopic shoot apical meristem (SAM) maize provide single-cell transcriptomic analysis a plant meristem. This study enabled an unbiased developmental genetic organization apex uncovered evolutionarily divergent conserved signatures SAM homeostasis. The...
Abstract Plant shoot organs arise from initial cells that are recruited meristematic tissues. Previous studies have shown members of the WUSCHEL-related HOMEOBOX (WOX) gene family function to organize various cell populations during plant development. The WOX4 is previously undescribed in any species. Comparative analyses transcription and presented Arabidopsis (Arabidopsis thaliana), a simple-leafed with collateral vasculature, tomato (Solanum lycopersicum), dissected-leafed species...
The narrow sheath (ns) phenotype of maize is a duplicate factor trait conferred by mutations at the unlinked loci ns1 and ns2. Recessive each locus together confer phenotypic deletion lateral compartment in leaves leaf homologs. Previous analyses revealed that mediolateral axis comprised least two distinct compartments, suggest model whereby NS function required to recruit founder cells from meristems. Genomic clones homeodomain-encoding genes were isolated homology WUSCHEL-related gene...
ABSTRACT The maize mutant narrow sheath (ns) displays a leaf shape and plant stature phenotype that suggests the preprimordial deletion of domain. ns is inherited as recessive, duplicate-factor trait, conditioned upon homozygosity for each two unlinked mutations sheath-1 (ns1) sheath-2 (ns2). Mutant leaves are missing large domain including margin, internodes shortened on marginal side stem. This extends from internode to beyond longitudinal mid-length blade, corresponds an alteration in...
INTRODUCTION Zea mays ssp. is one of the world’s most important crop plants, boasting a multibillion dollar annual revenue. In addition to its agronomic importance, maize has been keystone model organism for basic research nearly century. Within cereals, which include other plant species such as rice ( Oryza sativa ), sorghum Sorghum bicolor wheat Triticum spp.), and barley Hordeum vulgare thoroughly researched genetic system. Several attributes plant, including vast collection mutant...
How genes shape diverse plant and animal body forms is a key question in biology. Unlike cells, cells are confined by rigid cell walls, division plane orientation growth rather than movement determine overall form. The emergence of plants on land coincided with new capacity to rotate stem divisions through multiple planes, this enabled three-dimensional (3D) arise from ancestral constrained 2D growth. involved evolutionary innovation largely unknown. evolution 3D recapitulated during the...
MicroRNAs (miRNAs) and trans-acting siRNAs (ta-siRNAs) are essential to the establishment of adaxial–abaxial (dorsoventral) leaf polarity. Tas3-derived ta-siRNAs define adaxial side by restricting expression domain miRNA miR166, which in turn demarcates abaxial leaves determinants. To investigate regulatory mechanisms that allow for precise spatiotemporal accumulation these polarizing small RNAs, we used laser-microdissection coupled RT-PCR determine profiles their precursor transcripts...
The complex genomes of many economically important crops present tremendous challenges to understand the genetic control quantitative traits with great importance in crop production, adaptation, and evolution. Advances genomic technology need be integrated strategic design novel perspectives break new ground. Complementary individual-gene–targeted research, which remains challenging, a global assessment distribution trait-associated SNPs (TASs) discovered from genome scans can provide...
Abstract Development of multicellular organisms proceeds via the correct interpretation positional information to establish boundaries that separate developmental fields with distinct identities. The maize (Zea mays) leaf is an ideal system study plant morphogenesis as it subdivided into a proximal sheath and distal blade, each patterning. Specialized ligule auricle structures form at blade-sheath boundary. auricles act hinge, allowing blade project angle from stem, while comprises...
Abstract The maize shoot apical meristem (SAM) comprises a small pool of stem cells that generate all above-ground organs. Although mutational studies have identified genetic networks regulating SAM function, little is known about morphological variation in natural populations. Here we report the use high-throughput image processing to capture rich size within diverse inbred panel. We demonstrate correlations between seedling and agronomically important adult traits such as flowering time,...
Abstract Maize (Zea mays) leaves develop basipetally (tip to base); the upper blade emerges from shoot apical meristem (SAM) before expansion of lower sheath. Founder cells, leaf initials located in periphery SAM, are distinguished SAM proper by differential accumulation KNOX proteins. proteins accumulate but excluded maize primordia and founder cells. As Arabidopsis tomato (Lycopersicon esculentum), shoots failed initiate new when cultured polar auxin transport inhibitor...
All above-ground plant organs are derived from shoot apical meristems (SAMs). Global analyses of gene expression were conducted on maize (Zea mays L.) SAMs to identify genes preferentially expressed in the SAM. The collected 14-day-old B73 seedlings via laser capture microdissection (LCM). RNA samples extracted LCM-collected and hybridized microarrays spotted with 37 660 cDNAs. Approximately 30% (10 816) these cDNAs prepared as part this study manually dissected apices. Over 5000 sequence...
Abstract The WUSCHEL-related homeobox (WOX) gene PRESSED FLOWER1 (PRS1) performs a conserved function during lateral organ development in Arabidopsis (Arabidopsis thaliana). Expressed the periphery of shoot meristem, PRS1 recruits founder cells that form domains vegetative and floral organs. Null mutations cause deletion stipules from leaves sepals stamens flowers. Although expression is described L1 layer, all meristem layers. mechanism non-cell autonomous evolution disparate WOX functions...
Transposable elements (TEs) comprise a substantial portion of many eukaryotic genomes and are typically transcriptionally silenced. RNA–dependent RNA polymerase 2 (RDR2) is component the RNA–directed DNA methylation (RdDM) silencing pathway. In maize, loss mediator paramutation1 (mop1) encoded RDR2 function results in reactivation silenced Mu transposons reduction accumulation 24 nt short-interfering RNAs (siRNAs) that recruit components. An RNA–seq experiment conducted on shoot apical...
Transcriptome variation plays an important role in affecting the phenotype of organism. However, understanding underlying mechanisms regulating transcriptome segregating populations is still largely unknown. We sought to assess and map transcript abundance maize shoot apices intermated B73×Mo17 recombinant inbred line population. RNA–based sequencing (RNA–seq) allowed for detection quantification derived from 28,603 genes. For a majority these genes, population mean, coefficient variation,...
The maize (Zea mays) shoot apical meristem (SAM) arises early in embryogenesis and functions during stem cell maintenance organogenesis to generate all the aboveground organs of plant. Despite its integral role development, little is known about molecular mechanisms SAM initiation. Laser microdissection domains from developing embryos seedlings was combined with RNA sequencing for transcriptomic analyses ontogeny. Molecular markers key events are described, comprehensive transcriptional data...
Abstract Forward genetics remains a powerful method for revealing the genes underpinning organismal form and function, how these are tied together in gene networks. In maize, forward has been tremendously successful, but size complexity of maize genome made identifying mutant an often arduous process with traditional methods. The next generation sequencing revolution allowed cloning to be significantly accelerated many organisms, even when genomes large complex. Here, we describe...
There are significant limitations in existing methods for the genome-wide identification of genes whose expression patterns affect traits.The transcriptomes five tissues from 27 genetically diverse maize inbred lines were deeply sequenced to identify exhibiting high and low levels variation across or genotypes. Transcription factors enriched among with most tissues, as well higher-than-median In contrast, transcription depleted is either highly stable variable We developed a Bayesian-based...
Abstract Background and Aims Prior work has examined cuticle function, composition ultrastructure in many plant species, but much remains to be learned about how these features are related. This study aims elucidate relationships between via analysis of development adult maize (Zea mays L.) leaves, while also providing the most comprehensive investigation date leaf cuticles this important crop plant. Methods We water permeability, wax cutin gas chromatography, transmission electron...
Summary Shoot apical meristem ( SAM ) structure varies markedly within the land plants. The s of many seedless vascular plants contain a conspicuous inverted, pyramidal cell called AC ), which is unidentified in angiosperms. In this study, we use transcriptomic sequencing with precise laser microdissections subdomains to define molecular signatures anatomically distinct zones from ‐type lycophyte Selaginella moellendorffii and monilophyte Equisetum arvense ). two model species for study...