A5020722135- Plant and animal studies
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant Diversity and Evolution
- Plant Parasitism and Resistance
- Plant nutrient uptake and metabolism
- Plant and Fungal Species Descriptions
- Plant Gene Expression Analysis
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Intellectual Property and Patents
- Intellectual Property Law
- Open Source Software Innovations
- Plant biochemistry and biosynthesis
Harvard University
2020-2022
New York University
2014-2018
New York Botanical Garden
2015-2018
Cornell University
2015
Significance Our study exploits time—the relatively unexplored fourth dimension of gene regulatory networks (GRNs)—to learn the temporal transcriptional logic underlying dynamic nitrogen (N) signaling in plants. We introduce several conceptual innovations to analysis time-series data area predictive GRNs. resulting network now provides “transcriptional logic” for transcription factor perturbations aimed at improving N-use efficiency, an important issue global food production marginal soils...
Significance Cellular signals evoke rapid and broad changes in gene regulatory networks. To uncover these network dynamics, we developed an approach able to monitor primary targets of a transcription factor (TF) based solely on regulation, the absence detectable binding. This enabled us follow transient propagation nitrogen (N) nutrient signal as direct impact master TF Basic Leucine Zipper 1 (bZIP1). Unexpectedly, largest class that exhibit associations with bZIP1 is uniquely relevant...
Abstract Background Histone methylation modifies the epigenetic state of target genes to regulate gene expression in context developmental and environmental changes. Previously, we used a positive genetic screen identify an Arabidopsis mutant, cli186 , which was impaired carbon light signaling. Here, report deletion histone methyltransferase SDG8 this mutant (renamed sdg8-5 ), provides unique opportunity study global function specific within multicellular organism. Results To assess role...
Summary Shoot apical meristem ( SAM ) structure varies markedly within the land plants. The s of many seedless vascular plants contain a conspicuous inverted, pyramidal cell called AC ), which is unidentified in angiosperms. In this study, we use transcriptomic sequencing with precise laser microdissections subdomains to define molecular signatures anatomically distinct zones from ‐type lycophyte Selaginella moellendorffii and monilophyte Equisetum arvense ). two model species for study...
Significance Throughout evolutionary history, organisms have evolved features that allow them to interact with their environment in novel ways. When such lead increased rates of speciation a lineage, we call key innovations. Understanding the genetic and developmental changes involved origin innovations is particular interest. Here identify gene, POPOVICH , crucial development innovation, floral nectar spurs, columbine genus Aquilegia . While function orthologs other plant taxa suggests an...
Interactions with animal pollinators have helped shape the stunning diversity of flower morphologies across angiosperms. A common evolutionary consequence these interactions is that some flowers converged on suites traits, or pollination syndromes, attract and reward specific pollinator groups. Determining genetic basis floral syndromes can help us understand processes contributed to diversification Here, we characterize architecture a bee-to-hummingbird shift in Aquilegia (columbine) using...
Dynamic transcriptional regulation is critical for an organism's response to environmental signals and yet remains elusive capture. Such mediated by master transcription factors (TF) that control large gene regulatory networks. Recently, we described a dynamic mode of TF named "hit-and-run". This model proposes can interact transiently with set targets, but the these transient targets continues after dissociation from target promoter. However, experimental evidence validating active...
The paper presents an empirical analysis of the trademark registration practices 40 standards setting organizations. Key observations and conclusions include: (a) every organization had submitted a application with USPTO, all but four active registered marks, suggesting that trademarks play important role for SSOs; (b) nearly SSOs appear to be emphasizing their name as de facto “house mark” (although none formally apply status USPTO); (c) classes 9, 35 42 particularly – dominance class 9...
Abstract Premise Determining the developmental programs underlying morphological variation is key to elucidating evolutionary processes that generated stunning biodiversity of angiosperms. Here, we characterized and transcriptional dynamics elaborate petal nectar spur Aquilegia (columbine) in species with contrasting pollination syndromes morphologies. Methods We collected epidermal cell number length data across four species, two short, curved spurs bee‐pollination syndrome long, straight...
Abstract Interactions with animal pollinators have helped shape the stunning diversity of flower morphologies across angiosperms. A common evolutionary consequence these interactions is that some flowers converged on suites traits, or pollination syndromes, attract and reward specific pollinator groups. Determining genetic basis floral syndromes can help us understand processes contributed to diversification Here, we characterize architecture a bee-to-hummingbird shift in Aquilegia...
Abstract Floral organs are produced by floral meristems (FMs), which harbor stem cells in their centers. Since each flower only has a finite number of organs, the cell activity an FM will always terminate at specific time point, process termed meristem termination (FMT). Variation timing FMT can give rise to morphological diversity, but how this is fine-tuned developmental and evolutionary level poorly understood. Flowers from genus Aquilegia share identical organ arrangement except for...
ABSTRACT Floral organs are produced by floral meristems (FMs), which harbor stem cells in their centers. Since each flower only has a finite number of organs, the cell activity FM will always terminate at specific time point, process termed meristem termination (FMT). Variation timing FMT can give rise to morphological diversity, but how this is fine-tuned developmental and evolutionary level poorly understood. Flowers from genus Aquilegia share identical organ arrangement except for stamen...
Experimental design. (A) Vector maps of the pBeaconRFP construct containing 35S::GR::bZIP1 [6, 15] and pJD385 empty vector (EV) control a 35S::GR cassette. (B) A 20 minute exposure to 4tU was sufficient detect significant incorporation in RNA fractions Arabidopsis cells, as shown by dot blot imaging 1 μg from 4.5 106 cells exposed 1.5 mM (+) or uracil only (−). (PDF 36 kb)
ABSTRACT Premise Determining the developmental programs underlying morphological variation is key to elucidating evolutionary processes that generated stunning biodiversity of angiosperms. Here, we characterize and transcriptional dynamics elaborate petal nectar spur Aquilegia (columbine) in species with contrasting pollination syndromes morphologies. Methods We collected epidermal cell number length data across four species, two short, curved spurs bee-pollination syndrome, long, straight...